scholarly journals Influence of temperature on macromolecular composition of Escherichia coli

1969 ◽  
Author(s):  
Robert Paul Moore
2012 ◽  
Vol 15 (4) ◽  
pp. 797-798
Author(s):  
G. Wroński ◽  
K. Budzińska ◽  
B. Szejniuk ◽  
A. Jurek

Abstract The aim of the study was to determine the influence of temperature, i.e. 4 and 20oC on the Escherichia coli O157:H7 survival time in a stored cattle slurry in a laboratory model experiment. The results of this investigation indicated that the tested microorganisms underwent a gradual elimination in the cattle slurry, whereas their inactivation rate was clearly dependent on the temperature. A higher survival rate was found in Escherichia coli O157:H7 at 4oC where a theoretical survival time of these microorganisms, determined using a regression analysis, amounted to 83 days. Our study indicates that there is a necessity for the slurry to undergo hygienization processes and that a constant monitoring of liquid animal excreta in search for pathogenic microorganisms is required.


2017 ◽  
Vol 6 (1) ◽  
pp. 20-23 ◽  
Author(s):  
Md Shaheduzzaman ◽  
Md Sayedur Rahman ◽  
Ifra Tun Nur

The cellular response against environmental stresses is one of the most highly conserved regulatory features among all organisms. The exposure of cells to stresses such as heat shock leads to the accumulation of partially and fully denatured proteins that interfere with normal cellular function. Present study was designed to examine the growth and physiology of Escherichia coli at different temperatures in our laboratory condition. With a previous observation of Escherichia coli growth cessation by the increase in temperature on different culture media, current study further extended the examination of the influence of temperature on the growth behavior of fecal coliform on minimal media, a slight retardation in the colony and cell morphology was noticed for fecal coliform at 47 °C within 36 hours to 72 hours of incubation. Consistent result was also found in spot test for fecal species at 45°C.Stamford Journal of Microbiology, Vol.6(1) 2016: 20-23


1992 ◽  
Vol 108 (1) ◽  
pp. 115-121 ◽  
Author(s):  
J. Dolman ◽  
R. G. Board

SUMMARYThe inner membrane of the air cell of hens' eggs was inoculated with Pseudomonas putida, Staphylococcus xylosus, Enterococcus faecalis, Escherichia coli and Salmonella enteitidis.The first mentioned eventually dominated the contamination of the albumen of eggs stored at 4, 15, and 20 °C. The last mentioned did so in eggs stored at 37 °C. The interval between inoculation of the membrane and gross contamination of the albumen was markedly influenced by site of contamination relative to yolk movement.


1995 ◽  
Vol 58 (4) ◽  
pp. 352-356 ◽  
Author(s):  
SAMUEL A. PALUMBO ◽  
JEFFREY E. CALL ◽  
FRANKIE J. SCHULTZ ◽  
AARON C. WILLIAMS

The influence of temperature on growth and verotoxin production by Escherichia coli strains was studied in brain heart infusion (BHI) broth both in shake cultures at various temperatures and in a temperature-gradient incubator. All strains of E. coli surveyed grew from at least 10 to 45°C, with some strains growing at 8° C. Verotoxin production (determined using the Vero cell–assay system) was a function of both temperature and time, with the highest titers produced at temperatures supporting the fastest growth (based on days to visible turbidity) and highest viable cell counts. However, for strains producing verotoxin, toxin production was detected at any temperature supporting growth. Three strains (of 16 tested) increased 1000-fold in viable count in 4 to 6 days at 10°C. The data presented here indicate that most E. coli strains surveyed can easily grow at ca. 10°C and thus suggest the potential for growth in temperature-abused refrigerated foods.


Author(s):  
T. Geipel ◽  
W. Mader ◽  
P. Pirouz

Temperature affects both elastic and inelastic scattering of electrons in a crystal. The Debye-Waller factor, B, describes the influence of temperature on the elastic scattering of electrons, whereas the imaginary part of the (complex) atomic form factor, fc = fr + ifi, describes the influence of temperature on the inelastic scattering of electrons (i.e. absorption). In HRTEM simulations, two possible ways to include absorption are: (i) an approximate method in which absorption is described by a phenomenological constant, μ, i.e. fi; - μfr, with the real part of the atomic form factor, fr, obtained from Hartree-Fock calculations, (ii) a more accurate method in which the absorptive components, fi of the atomic form factor are explicitly calculated. In this contribution, the inclusion of both the Debye-Waller factor and absorption on HRTEM images of a (Oll)-oriented GaAs crystal are presented (using the EMS software.Fig. 1 shows the the amplitudes and phases of the dominant 111 beams as a function of the specimen thickness, t, for the cases when μ = 0 (i.e. no absorption, solid line) and μ = 0.1 (with absorption, dashed line).


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