Aqueous extract of Lespedeza cuneata improves male menopause by increase of nitric oxide and dihydrotestosterone

Purpose: To analyze the effects of an aqueous extract of Sericea lespedeza (SL) on rat male menopause.Methods: Levels of nitric oxide (NO), endothelial nitric oxide synthase (NOS), cGMP, and prostaglandin E2 (PGE2) in the penile corpus cavernosum of the rats were evaluated using appropriate kits. Serum levels of dihydrotestosterone (DHT), testosterone, sex hormone-binding globulin (SHBG), and 17-beta hydroxysteroid dehydrogenases (17β-HSD) were measured with enzyme-linked immunosorbent assay kits. Total and motile sperms were counted on a hemocytometer. Histological changes in rat testis and epididymis were analyzed with hematoxylin and eosin staining.Results: The levels of NO, NOS, and cGMP (but not PGE2) increased in a dose-dependent manner (p< 0.05) upon administration of an aqueous extract of SL (AESL), while levels of DHT, 17β-HSD, and testosterone increased in the group administered with 300 mg/kg of AESL. Epididymal sperm count increased by 24 % in such rats compared to controls (p < 0.05).Conclusion: The aqueous extract of SL improves sperm count and muscle mass in rats by increasing the levels of NO, NOS, cGMP and testosterone. Thus, SL extract can potentially be developed as an alternative therapeutic agent for clinical management of TDS. Keywords: NO-cGMP, Testosterone, Hormones, Sperm count, Muscle mass, Sericea lespedeza, Lespedeza cuneata

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Thein vitro effect of aqueous extract ofNigella sativa seeds on nitric oxide production

Phytotherapy Research ◽

10.1002/ptr.1251 ◽

2003 ◽

Vol 17 (8) ◽

pp. 921-924 ◽

Cited By ~ 49

Author(s):

M. Saeed Mahmood ◽

A. H. Gilani ◽

Afsheen Khwaja ◽

Ayesha Rashid ◽

M. K. Ashfaq

Keyword(s):

Nitric Oxide ◽

Aqueous Extract ◽

Nitric Oxide Production ◽

Oxide Production ◽

Vitro Effect

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Involvement of Heme Oxygenase-1 Participates in Anti-Inflammatory and Analgesic Effects of Aqueous Extract ofHibiscus taiwanensis

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/132859 ◽

2012 ◽

Vol 2012 ◽

pp. 1-13 ◽

Cited By ~ 8

Author(s):

Shu-Ling Liu ◽

Jeng-Shyan Deng ◽

Chuan-Sung Chiu ◽

Wen-Chi Hou ◽

Shyh-Shyun Huang ◽

...

Keyword(s):

Nitric Oxide ◽

Western Blotting ◽

Heme Oxygenase ◽

Aqueous Extract ◽

Heme Oxygenase 1 ◽

Natural Health Products ◽

Paw Edema ◽

Raw264.7 Macrophages ◽

Cox 2 ◽

Anti Inflammatory

Anti-inflammatory effects of the aqueous extract ofHibiscus taiwanensis(AHT) were used in lipopolysaccharide (LPS-)stimulated mouse macrophage RAW264.7 cells and carrageenan (Carr-)induced mouse paw edema model. When RAW264.7 macrophages were treated with AHT together with LPS, a concentration-dependent inhibition of nitric oxide (NO), tumor necrosis factor (TNF-α), and prostaglandin E2 (PGE2) levels productions were detected. Western blotting revealed that AHT blocked protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and elevated heme oxygenase-1 (HO-1), significantly. In the animal test, AHT decreased the paw edema at the 4th and the 5th h after Carr administration, and it increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the paw tissue. We also demonstrated AHT decreased the NO, TNF-α, and PGE2 levels on the serum level at the 5th h after the Carr injection. Western blotting revealed that AHT decreased Carr-induced iNOS, and COX-2, and increased HO-1 expressions at the 5th h in the edema paw. These findings demonstrated that AHT has excellent anti-inflammatory activitiesin vitroandin vivoand thus it has great potential to be used as a source for natural health products.

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Effects of an aqueous extract of cocoa on nitric oxide production of macrophages activated by lipopolysaccharide and interferon-γ

Nutrition ◽

10.1016/s0899-9007(03)00092-3 ◽

2003 ◽

Vol 19 (7-8) ◽

pp. 681-685 ◽

Cited By ~ 20

Author(s):

Kazuyuki Ono ◽

Toshio Takahashi ◽

Masanori Kamei ◽

Takashi Mato ◽

Shuichi Hashizume ◽

...

Keyword(s):

Nitric Oxide ◽

Aqueous Extract ◽

Interferon Γ ◽

Nitric Oxide Production ◽

Oxide Production

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Inhibition of nitric oxide release by an aqueous extract ofTinospora tuberculata

Phytotherapy Research ◽

10.1002/(sici)1099-1573(200002)14:1<51::aid-ptr545>3.0.co;2-k ◽

2000 ◽

Vol 14 (1) ◽

pp. 51-53 ◽

Cited By ~ 6

Author(s):

Takako Yokozawa ◽

Tian Shan Wang ◽

Cui Ping Chen ◽

Masao Hattori

Keyword(s):

Nitric Oxide ◽

Aqueous Extract ◽

Nitric Oxide Release

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Relaxant Effects of the Aqueous Extract of Excoecaria grahamii (Euphorbiaceae) Leaves on Uterine Horn Contractility in Wistar Rats

BioMed Research International ◽

10.1155/2021/6618565 ◽

2021 ◽

Vol 2021 ◽

pp. 1-8

Author(s):

Prosper A. Dabiré ◽

Youssoufou Ouédraogo ◽

Abel A. Somé ◽

Stanislas Sawadogo ◽

Issaka Ouédraogo ◽

...

Keyword(s):

Nitric Oxide ◽

Smooth Muscle ◽

Preterm Birth ◽

Aqueous Extract ◽

Wistar Rats ◽

Uterine Horn ◽

Uterine Smooth Muscle ◽

Uterine Contractions ◽

H2 Receptors ◽

Nitric Oxide Pathway

In uterine smooth muscle, the effects of Excoecaria grahamii are not yet documented. To fill this gap, we investigated the pharmacological effect of Excoecaria grahamii on the contraction of the rat isolated uterine horns. The isolated segments were exposed to different concentrations of the aqueous extract of Excoecaria grahamii leaves and pharmacological drugs. The results showed that Excoecaria grahamii aqueous extract decreased the amplitude and frequency by concentration-related manner. I C 50 values were 2.4 and 2.6, respectively, for amplitude and frequency. Our study revealed that the extract did not act through histamine H2-receptors or the nitric oxide pathway. It also inhibited uterine contractions induced by oxytocin and potassium chloride (KCl). These data suggest that Excoecaria grahamii active compound can be used for calming uterine contractions. The action of Excoecaria grahamii showed that it can be useful to fight against diseases which caused uterotonic effects. It can be useful to prevent preterm birth and pains caused by menstruations but further investigation is needed to clarify the mechanism action.

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Leaves Aqueous Extract of <i>Boerhavia coccinea</i> Induces Antinociceptive Effect but Increases Nitric Oxide Production in Macrophages

Journal of Biosciences and Medicines ◽

10.4236/jbm.2020.810009 ◽

2020 ◽

Vol 08 (10) ◽

pp. 90-103

Author(s):

Basile N. Piegang ◽

Douglas A. M. Ngangoum ◽

Sorelle M. Ngassam ◽

Pius P. Tseuguem ◽

Bibiane A. Wandji ◽

...

Keyword(s):

Nitric Oxide ◽

Aqueous Extract ◽

Antinociceptive Effect ◽

Nitric Oxide Production ◽

Oxide Production

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A Comparative Study of Antioxidant Activity between Ethanolic and Aqueous Extract of Cardiospermum halicacabum

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00820 ◽

2021 ◽

pp. 4715-4718

Author(s):

Kumudhavalli M.V. ◽

Nandhinipriya V.

Keyword(s):

Nitric Oxide ◽

Antioxidant Activity ◽

Ascorbic Acid ◽

Hydroxyl Radical ◽

Aqueous Extract ◽

Research Work ◽

Assay Method ◽

Vitro Assay ◽

Aqueous Solvent ◽

Dpph Method

Cardiospermum halicacabum is conventionally used in the treatment of stiffness of limbs, chronic bronchitis, and snakebites. The whole plant of Cardiospermum halicacabum has been extracted using ethanol and aqueous solvent and estimation of their antioxidant activity done for predicted current research work. Both ethanol and aqueous extracts illustrate important antioxidant activity in the DPPH method, nitric oxide method, and hydroxyl radical-induced In-vitro assay method. The DPPH radical inhibition (%) was 53.13, 57.13, and 79.12 for EECH, AECH, and Standard ascorbic acid respectively. The Cardiospermum halicacabum extracts (EECH and AECH) showed significant free radical scavenging action against nitric oxide (NO) induced release of free radicals at the concentrations 250μg/ml, showing 29.22% and 63.32% of nitric oxide inhibition, respectively. The EECH and AECH extracts (25-400μg/ml) significantly scavenged the hydroxyl radical generated by the EDTA/HO system, when compared to that of ascorbic acid. The plant Cardiospermum halicacabum has been used to treat different ailments. The present study clearly showed that both the extracts viz. EECH and AECH of the plant possess significant antioxidant activity. Moreover, ethanolic extract was proved as more potent candidature as compared to aqueous extract. The molecular level mechanism is the future interest of the researchers to prove the traditional use of the plant.

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Effect of Peganum Harmala Seeds Extract on Nitric Oxide in U937 Monocytes and Macrophages

International Journal of Medical Laboratory ◽

10.18502/ijml.v7i4.4803 ◽

2020 ◽

Author(s):

Nima Rahmati ◽

Fatemeh Hajighasemi

Keyword(s):

Nitric Oxide ◽

Aqueous Extract ◽

Calf Serum ◽

Peritoneal Macrophages ◽

No Production ◽

Peganum Harmala ◽

Logarithmic Growth Phase ◽

Anti Inflammatory

Background and Aims: Nitric oxide (NO) has an essential role in inflammation and has been related to pathogenesis and the progress of numerous inflammatory-based diseases, including some cancers. Peganum harmala (P. harmala) is a medicinal plant used for the treatment of numerous diseases such as several infections. Also, anti-inflammatory effects of P. harmala extracts and its derivatives (harmaline and harmine) by suppressing myeloperoxidase, NO, and other mediators have been demonstrated in vivo. In this study, the effect of P. harmala seeds aqueous extract on NO production in U937 monocytic cells and peritoneal macrophages has been evaluated in vitro. Materials and Methods: U937 and mice peritoneal macrophages were cultured in Roswell Park Memorial institute-1640 with 10% fetal calf serum. Then, the cells at the logarithmic growth phase were incubated with different concentrations of aqueous extract of P. harmala seeds (0.1-1 mg/ml) for 24 hours. Next, NO production was assessed by the Griess method in the culture medium. Results: P. harmala seeds aqueous extract did not significantly affect lipopolysaccharide-induced NO production in U937 cells and peritoneal macrophages after 24 hours incubation time compared with untreated control cells. Conclusion: These results suggest that the anti-inflammatory effects of P. harmala may be mediated through NO-independent mechanism(s). However, further studies are warranted to define the P. harmala aqueous extract impact on NO expression in other related normal and cancerous cells.

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