scholarly journals Relaxant Effects of the Aqueous Extract of Excoecaria grahamii (Euphorbiaceae) Leaves on Uterine Horn Contractility in Wistar Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Prosper A. Dabiré ◽  
Youssoufou Ouédraogo ◽  
Abel A. Somé ◽  
Stanislas Sawadogo ◽  
Issaka Ouédraogo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Preterm Birth ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Uterine Horn ◽  
Uterine Smooth Muscle ◽  
Uterine Contractions ◽  
H2 Receptors ◽  
Nitric Oxide Pathway

In uterine smooth muscle, the effects of Excoecaria grahamii are not yet documented. To fill this gap, we investigated the pharmacological effect of Excoecaria grahamii on the contraction of the rat isolated uterine horns. The isolated segments were exposed to different concentrations of the aqueous extract of Excoecaria grahamii leaves and pharmacological drugs. The results showed that Excoecaria grahamii aqueous extract decreased the amplitude and frequency by concentration-related manner. I C 50 values were 2.4 and 2.6, respectively, for amplitude and frequency. Our study revealed that the extract did not act through histamine H2-receptors or the nitric oxide pathway. It also inhibited uterine contractions induced by oxytocin and potassium chloride (KCl). These data suggest that Excoecaria grahamii active compound can be used for calming uterine contractions. The action of Excoecaria grahamii showed that it can be useful to fight against diseases which caused uterotonic effects. It can be useful to prevent preterm birth and pains caused by menstruations but further investigation is needed to clarify the mechanism action.

Increased expression of nitric oxide synthase in the myometrium of the pregnant rat uterus

1997 ◽  
Vol 272 (6) ◽  
pp. E1008-E1015 ◽  
Author(s):  
R. K. Riemer ◽  
C. Buscher ◽  
R. K. Bansal ◽  
S. M. Black ◽  
Y. He ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
No Synthase ◽  
Inos Expression ◽  
Rat Uterus ◽  
Pregnant Rat ◽  
Pregnant Uterus ◽  
Uterine Smooth Muscle ◽  
Uterine Contractions ◽  
Oxide Synthase

Nitric oxide (NO) relaxes uterine smooth muscle and is produced by the pregnant uterus. Our previous studies revealed an increase in rat uterine NO synthase (NOS) activity in pregnancy and a decline at term. In the present study, we have examined the distribution of NOS isoform expression to determine whether their regulation is consistent with a role in the inhibition of uterine contractions before term. At day 17-18 of pregnancy, NOS immunohistochemistry revealed expression of two isoforms: endothelial constitutive form of NOS (ecNOS) in vascular endothelium and inducible form of NOS (iNOS) in myometrial and vascular smooth muscle and in decidual epithelium. Immunoblotting revealed that expression of iNOS declined nearly fivefold, whereas ecNOS declined twofold in laboring rats at term. We conclude that iNOS is expressed in myometrium of pregnant rat uterus but not the virgin rat and that iNOS expression declines at term when labor is present. The pattern of changes in myometrial iNOS expression with advancing gestation suggests that NO could act in an autocrine and/or paracrine manner to inhibit uterine contractions before term.

scholarly journals Mesenchymal Stem Cells Attenuate Lipopolysaccharide-Induced Inflammatory Response in Human Uterine Smooth Muscle Cells

2020 ◽  
Vol 10 (03) ◽  
pp. e335-e341
Author(s):  
Arunmani Mani ◽  
John W. Hotra ◽  
Sean C. Blackwell ◽  
Laura Goetzl ◽  
Jerrie S. Refuerzo
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Preterm Birth ◽  
Inflammatory Response ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Uterine Smooth Muscle ◽  
Cell Based Therapy ◽  
Increased Risk

Abstract Objective The aim of this study was to determine if mesenchymal stem cells (MSCs) would suppress the inflammatory response in human uterine cells in an in vitro lipopolysaccharide (LPS)-based preterm birth (PTB) model. Study Design Cocultures of human uterine smooth muscle cells (HUtSMCs) and MSCs were exposed to 5 μg/mL LPS for 4 hours and further challenged with 1 μg/mL LPS for a subsequent 24 hours. Key elements of the parturition cascade regulated by toll-like receptors (TLRs) through activation of mitogen-activated protein kinases (MAPKs) were quantified in culture supernatant as biomarkers of MSC modulation. Results Coculture with MSCs significantly attenuated TLR-4, p-JNK, and p- extracellular signal-regulated kinase 1/2 (ERK1/2) protein levels compared with HUtSMCs monoculture (p = 0.05). In addition, coculture was associated with significant inhibition of proinflammatory cytokines interleukin (IL)-6 and IL-8 (p = 0.0001) and increased production of anti-inflammatory cytokines IL-10 and transforming growth factor (TGF)-β1 (p = 0.0001). Conclusion MSCs appear to play a role in significantly attenuating LPS-mediated inflammation via alteration of down-stream MAPKs. MSCs may represent a novel, cell-based therapy in women with increased risk of inflammatory-mediated preterm birth.

THE NITRIC OXIDE PATHWAY IN PIG CALYCEAL SMOOTH MUSCLE

1999 ◽  
pp. 81
Author(s):  
Christophe E. Iselin ◽  
Lars Ny ◽  
Emanuela Felley-Bosco ◽  
Bengt Larsson ◽  
Dominique Mastrangelo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Nitric Oxide Pathway

The nitric oxide pathway in pig isolated calyceal smooth muscle

1999 ◽  
Vol 18 (6) ◽  
pp. 673-685 ◽  
Author(s):  
Christophe E. Iselin ◽  
Lars Ny ◽  
Dominique Mastrangelo ◽  
Emanuela Felley-Bosco ◽  
Bengt Larsson ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Nitric Oxide Pathway

scholarly journals Endothelium-Independent Relaxation of Vascular Smooth Muscle Induced by Persimmon-Derived Polyphenol Phytocomplex in Rats

Nutrients ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 89
Author(s):  
Risa Kudo ◽  
Katsuya Yuui ◽  
Shogo Kasuda
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Mesenteric Arteries ◽  
Inositol Triphosphate ◽  
Sprague Dawley ◽  
Vasorelaxant Effect ◽  
Sprague Dawley Rats ◽  
The Mean ◽  
Artery Disease ◽  
Nitric Oxide Pathway

The vasorelaxant effect of polyphenols is well known, and the mortality rate due to coronary artery disease is low in people who consume polyphenol-containing foods. We aimed to elucidate the mechanism by which polyphenols derived from persimmon juice (PJ) and persimmon leaves (PLs) induce vasorelaxation and suppress vasocontraction in the superior mesenteric arteries isolated from male Sprague Dawley rats. Vasocontraction was induced with 1 µM phenylephrine, and polyphenol-induced vasorelaxation was expressed as a percentage of the previous tone induced by phenylephrine. PJ powder (100 mg/L) induced higher levels of vasorelaxation (mean ± standard error of the mean, 88.6% ± 4.4%) than PLs powder (1 g/L; 72.0% ± 10.8%). Nitric oxide pathway inhibitors (NG-nitro-L-arginine methyl ester + carboxy-PTIO) did not affect persimmon-derived polyphenol-induced vasorelaxation, whereas potassium chloride, tetraethylammonium, and potassium-channel inhibitors did. Vasorelaxation was endothelium independent with both extracts. Phenylephrine-induced vasocontraction was suppressed by pretreatment with PJ and PLs powder, even when inositol triphosphate-mediated Ca2+ release and extracellular Ca2+ influx were inhibited. These results suggest that persimmon-derived polyphenol phytocomplex cause vasorelaxation and inhibit vasocontraction through hyperpolarization of smooth muscle cells. Persimmon-derived polyphenols may be able to prevent cardiovascular diseases caused by abnormal contraction of blood vessels.

scholarly journals The human uterine smooth muscle S-nitrosoproteome fingerprint in pregnancy, labor, and preterm labor

2013 ◽  
Vol 305 (8) ◽  
pp. C803-C816 ◽  
Author(s):  
Craig Ulrich ◽  
David R. Quilici ◽  
Karen A. Schlauch ◽  
Iain L. O. Buxton
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Preterm Delivery ◽  
Preterm Labor ◽  
Molecular Mechanisms ◽  
Area Under The Curve ◽  
Soluble Guanylyl Cyclase ◽  
The United States ◽  
Nitric Oxide Donor ◽  
Uterine Smooth Muscle

Molecular mechanisms involved in uterine quiescence during gestation and those responsible for induction of labor at term are incompletely known. More than 10% of babies born worldwide are premature and 1,000,000 die annually. Preterm labor results in preterm delivery in 50% of cases in the United States explaining 75% of fetal morbidity and mortality. There is no Food and Drug Administration-approved treatment to prevent preterm delivery. Nitric oxide-mediated relaxation of human uterine smooth muscle is independent of global elevation of cGMP following activation of soluble guanylyl cyclase. S-nitrosation is a likely mechanism to explain cGMP-independent relaxation to nitric oxide and may reveal S-nitrosated proteins as new therapeutic targets for the treatment of preterm labor. Employing S-nitrosoglutathione as an nitric oxide donor, we identified 110 proteins that are S-nitrosated in 1 or more states of human pregnancy. Using area under the curve of extracted ion chromatograms as well as normalized spectral counts to quantify relative expression levels for 62 of these proteins, we show that 26 proteins demonstrate statistically significant S-nitrosation differences in myometrium from spontaneously laboring preterm patients compared with nonlaboring patients. We identified proteins that were up- S-nitrosated as well as proteins that were down- S-nitrosated in preterm laboring tissues. Identification and relative quantification of the S-nitrosoproteome provide a fingerprint of proteins that can form the basis of hypothesis-directed efforts to understand the regulation of uterine contraction-relaxation and the development of new treatment for preterm labor.

scholarly journals Effect of Allanblackia floribunda stem bark aqueous extract in male albino wistar rats: androgenic activities and penile nitric oxide (NO) production

2021 ◽  
Vol 7 (2) ◽  
pp. 63-73
Author(s):  
Antoine Kada Sanda ◽  
David Fotsing ◽  
Oumar Mahamat ◽  
Vijayakumar Natesan ◽  
Jayaprakash Rajendran ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Stem Bark ◽  
No Production

scholarly journals Nitric oxide as a possible regulator of energy-dependent Ca2+ transport in mitochondria of uterine smooth muscle

2014 ◽  
Vol 60 (2) ◽  
pp. 12-17 ◽  
Author(s):  
IuV Danylovych ◽  
◽  
OV Kolomiiets' ◽  
HV Danylovych ◽  
SO Kosterin ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Smooth Muscle ◽  
Uterine Smooth Muscle ◽  
Energy Dependent ◽  
Transport In Mitochondria
Sign in / Sign up

Export Citation Format

Share Document