Rapid detection of the Clostridium difficile ribotype 027 tcdC gene frame shift mutation at position 117 by real-time PCR and melt curve analysis

2009 ◽  
Vol 28 (8) ◽  
pp. 959-962 ◽  
Author(s):  
D. Wolff ◽  
T. Brüning ◽  
A. Gerritzen
Keyword(s):  
Clostridium Difficile ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Curve Analysis ◽  
Melt Curve Analysis ◽  
Ribotype 027 ◽  
Frame Shift ◽  
Frame Shift Mutation

scholarly journals Rapid Detection and Identification of Metallo- -Lactamase-Encoding Genes by Multiplex Real-Time PCR Assay and Melt Curve Analysis

2006 ◽  
Vol 45 (2) ◽  
pp. 544-547 ◽  
Author(s):  
R. E. Mendes ◽  
K. A. Kiyota ◽  
J. Monteiro ◽  
M. Castanheira ◽  
S. S. Andrade ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Curve Analysis ◽  
Pcr Assay ◽  
Melt Curve Analysis ◽  
Detection And Identification ◽  
Encoding Genes

Evaluation of different direct and indirect SELEX monitoring methods and implementation of melt-curve analysis for rapid discrimination of variant aptamer sequences

2020 ◽  
Vol 12 (30) ◽  
pp. 3823-3835
Author(s):  
Rezvan Mohammadinezhad ◽  
Seyed Amir Hossein Jalali ◽  
Hamid Farahmand
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Accurate Method ◽  
Curve Analysis ◽  
Monitoring Methods ◽  
Protein Targets ◽  
Melt Curve Analysis ◽  
Rapid Discrimination

EMSA is the most cost-beneficial and accurate method for screening SELEX for protein targets and melt curve analysis in colony real-time PCR is a robust and effective method for distingsuishing variant aptamer sequences.

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