A microfluidic-based system using reverse transcription polymerase chain reactions for rapid detection of aquaculture diseases

2009 ◽  
Vol 7 (6) ◽  
pp. 795-806 ◽  
Author(s):  
Kang-Yi Lien ◽  
Szu-Hsien Lee ◽  
Tieh-Jung Tsai ◽  
Tzong-Yueh Chen ◽  
Gwo-Bin Lee
Keyword(s):  
Reverse Transcription ◽  
Rapid Detection ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Polymerase Chain

scholarly journals Characteristic Differences in Reverse Transcription-Polymerase Chain Reaction Products of Ovine, Bovine, and Human Respiratory Syncytial Viruses

1993 ◽  
Vol 5 (3) ◽  
pp. 322-328 ◽  
Author(s):  
Richard D. Oberst ◽  
Michael P. Hays ◽  
Jim F. Evermann ◽  
Clayton L. Kelling
Keyword(s):  
Reverse Transcription ◽  
Reaction Products ◽  
Rt Pcr ◽  
Specific Primers ◽  
F Protein ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Respiratory Syncytial Viruses ◽  
Polymerase Chain ◽  
Syncytial Virus

In reverse transcription-polymerase chain reactions (RT-PCR) and DNA hybridizations using primers and an oligonucleotide probe to the fusion (F) protein mRNA of bovine respiratory syncytial virus (BRSV), all the BRSV isolates and a goat isolate could be distinguished from prototype isolates of human respiratory syncytial viruses (HRSV) and ovine (sheep and bighorn sheep) respiratory syncytial viruses (RSV). However, RT-PCR amplifications with primers to sequences of the HRSV F protein mRNA resulted in amplified products of ≍ 243 bp if mRNA templates of subgroup A HRSV strains were present and slightly larger amplified products with subgroup B HRSV strains. No amplified products were observed in HRSV-primed RT-PCR with BRSV or goat or ovine RSV mRNA templates. Although the ovine RSV isolates were antigenically cross-reactive with the goat RSV, HRSV and BRSV isolates, they were not amplified with either HRSV- or BRSV-specific primers in RT-PCR. These results confirm previous immunological comparisons suggesting that some ovine RSV isolates should be considered as distinct respiratory syncytial viruses.

Rapid Detection and Quantification of Lanthanide Ions Using High-Speed Polymerase Chain Reactions

2014 ◽  
Vol 875-877 ◽  
pp. 749-754
Author(s):  
Asuka Kikuchi ◽  
Takashi Kadono ◽  
Kazuhiro Hashimoto ◽  
Kazuya Uezu ◽  
Tomonori Kawano
Keyword(s):  
Rapid Detection ◽  
High Speed ◽  
High Sensitivity ◽  
Lanthanide Ions ◽  
Quantitative Detection ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Novel Approach ◽  
Highly Sensitive ◽  
Polymerase Chain

Recently, a novel approach to a highly sensitive and quantitative detection of rare earth element (REE) ions including La3+, Eu3+ and Tb3+, by the polymerase chain reaction (PCR) technique, has been reported. The detection of REE ions is based on the catalytic nature of REE ions targeting the deoxyribonucleic acid (DNA), thus monitoring of the ions can be achieved by reading the level of intact DNA by PCR. Despite of its high sensitivity (at ppb to ppt levels), the conventional PCR-based REE detection protocol requires certain length of time (1-2 hours). In the present study, we modified the PCR-based REE detection protocols by employing the high-speed PCR, and performed the automated and rapid detection of La3+ in small-sized aqueous samples within 5min.

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