Reactive oxygen species and imbalance of calcium homeostasis contributes to curcumin induced programmed cell death in Leishmania donovani

Programmed cell death (PCD) plays crucial roles in plant development and defence response. Reactive oxygen species (ROS) are produced during normal plant growth, and high ROS concentrations can change the antioxidant status of cells, leading to spontaneous cell death. In addition, ROS function as signalling molecules to improve plant stress tolerance, and they induce PCD under different conditions. This review describes the mechanisms underlying plant PCD, the key functions of mitochondria and chloroplasts in PCD, and the relationship between mitochondria and chloroplasts during PCD. Additionally, the review discusses the factors that regulate PCD. Most importantly, in this review, we summarise the sites of production of ROS and discuss the roles of ROS that not only trigger multiple signalling pathways leading to PCD but also participate in the execution of PCD, highlighting the importance of ROS in PCD.

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Rosmarinic acid induces programmed cell death in Arabidopsis seedlings through reactive oxygen species and mitochondrial dysfunction

PLoS ONE ◽

10.1371/journal.pone.0208802 ◽

2018 ◽

Vol 13 (12) ◽

pp. e0208802 ◽

Cited By ~ 13

Author(s):

Fabrizio Araniti ◽

Aitana Costas-Gil ◽

Luz Cabeiras-Freijanes ◽

Antonio Lupini ◽

Francesco Sunseri ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Cell Death ◽

Programmed Cell Death ◽

Mitochondrial Dysfunction ◽

Rosmarinic Acid ◽

Reactive Oxygen ◽

Oxygen Species

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Nitric Oxide Enhances Cytotoxicity of Lead by Modulating the Generation of Reactive Oxygen Species and Is Involved in the Regulation of Pb2+ and Ca2+ Fluxes in Tobacco BY-2 Cells

Plants ◽

10.3390/plants8100403 ◽

2019 ◽

Vol 8 (10) ◽

pp. 403 ◽

Cited By ~ 2

Author(s):

Jiaye Wu ◽

Yue Zhang ◽

Ruizhi Hao ◽

Yuan Cao ◽

Xiaoyi Shan ◽

...

Keyword(s):

Nitric Oxide ◽

Reactive Oxygen Species ◽

Heavy Metal ◽

Cell Death ◽

Calcium Homeostasis ◽

Reactive Oxygen ◽

Ros Generation ◽

Plant Responses ◽

Oxygen Species ◽

In Cells

Lead is a heavy metal known to be toxic to both animals and plants. Nitric oxide (NO) was reported to participate in plant responses to different heavy metal stresses. In this study, we analyzed the function of exogenous and endogenous NO in Pb-induced toxicity in tobacco BY-2 cells, focusing on the role of NO in the generation of reactive oxygen species (ROS) as well as Pb2+ and Ca2+ fluxes using non-invasive micro-test technology (NMT). Pb treatment induced BY-2 cell death and rapid NO and ROS generation, while NO burst occurred earlier than ROS accumulation. The elimination of NO by 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) resulted in a decrease of ROS, and the supplementation of NO by sodium nitroprusside (SNP) caused an increased accumulation of ROS. Furthermore, the addition of exogenous NO stimulated Pb2+ influx, thus promoting Pb uptake in cells and aggravating Pb-induced toxicity in cells, whereas the removal of endogenous NO produced the opposite effect. Moreover, we also found that both exogenous and endogenous NO enhanced Pb-induced Ca2+ effluxes and calcium homeostasis disorder. These results suggest that exogenous and endogenous NO played a critical regulatory role in BY-2 cell death induced by Pb stress by promoting Pb2+ influx and accumulation and disturbing calcium homeostasis.

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