Activation of Na+-K+-ATPase with DRm217 attenuates oxidative stress-induced myocardial cell injury via closing Na+-K+-ATPase/Src/Ros amplifier

APOPTOSIS ◽  
2017 ◽  
Vol 22 (4) ◽  
pp. 531-543 ◽  
Author(s):  
Xiaofei Yan ◽  
Meng Xun ◽  
Xiaojuan Dou ◽  
Litao Wu ◽  
Fujun Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Injury ◽  
Myocardial Cell ◽  
Myocardial Cell Injury

scholarly journals Anshen Buxin Liuwei Pill, a Mongolian Medicinal Formula, Could Protect H2O2-Induced H9c2 Myocardial Cell Injury by Suppressing Apoptosis, Calcium Channel Activation, and Oxidative Stress

2022 ◽  
Vol 2022 ◽  
pp. 1-11
Author(s):  
Yue Dong ◽  
Hai-Ying Tong ◽  
Xian-Ju Huang ◽  
Ghulam Murtaza ◽  
Yu-Jia Huang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Injury ◽  
Cardiac Injury ◽  
Myocardial Cell ◽  
H9c2 Cell ◽  
Mrna Levels ◽  
H9c2 Cells ◽  
Therapeutic Benefits ◽  
Myocardial Cell Injury ◽  
And Oxidative Stress

Background. Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula which has a therapeutic effect on the symptoms such as coronary heart disease, angina pectoris, arrhythmia, depression and irritability, palpitation, and short breath. However, its bioactivity against cardiac injury remains unclear. Methods. The protective effect of ABLP was evaluated using H9c2 cells. Cell viability, intracellular Ca2+, reactive oxidative indices, and mitochondrial membrane potential (∆ψ) were assessed, respectively. The mRNA levels of Ca2+ channel-related genes (DHPR, RyR2, and SCN5A) and oxidative stress-related genes (Keap1, Nrf2, and HO-1) were measured by RT-PCR. Results. 0.5–50 μg/mL ABLP could significantly decrease H2O2-induced cell injury by suppressing cell necrosis/apoptosis and excess oxidative stress, ameliorating the collapse of ∆ψ, and reducing intracellular Ca2+ concentration. Furthermore, 0.5–50 μg/mL ABLP reversed H2O2-induced imbalance in the mRNA levels of DHPR, RyR2, SCN5A, Keap1, Nrf2, and HO-1 gene in H9c2 cells, which further illustrate the mechanism. Conclusion. ABLP provided protective and therapeutic benefits against H2O2-induced H9c2 cell injury, indicating that this formula can effectively treat coronary disease. In addition, the present study also provides an in-depth understanding of the pharmacological functions of ABLP, which may lead to further successful applications of Mongolian medicine.

scholarly journals Perioperative myocardial cell injury: the role of troponins

1997 ◽  
Vol 78 (4) ◽  
pp. 386-390 ◽  
Author(s):  
H Metzler ◽  
M Gries ◽  
P Rehak ◽  
T Lang ◽  
S Fruhwald ◽  
...  
Keyword(s):  
Cell Injury ◽  
Myocardial Cell ◽  
Myocardial Cell Injury

Cytosolic Free Calcium and Myocardial Cell Injury

1988 ◽  
pp. 413-420 ◽  
Author(s):  
E. Murphy ◽  
C. Steenbergen ◽  
A. LeFurgey ◽  
M. Lieberman ◽  
R. E. London
Keyword(s):  
Cell Injury ◽  
Myocardial Cell ◽  
Free Calcium ◽  
Cytosolic Free Calcium ◽  
Myocardial Cell Injury
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