Effects of Immunization with Group A Streptococcal Antigens on the Transplantability of Mouse Bone Marrow Stromal Stem Cells, Counts of Stromal Precursor Cells, and Their Osteogenic Characteristics

: The isolation and culture of murine bone marrow-derived mesenchymal stromal stem cells (mBMSCs) have attracted great interest in terms of the pre-clinical applications of stem cells in tissue engineering and regenerative medicine. In addition, culturing mBMSCs is important for studying the molecular mechanisms of bone remodelling using relevant transgenic mice. Several factors have created challenges in the isolation and high-yield expansion of homogenous mBMSCs; these factors include low frequencies of bone marrow-derived mesenchymal stromal stem cells (BMSCs) in bone marrow, variation among inbred mouse strains, contamination with haematopoietic progenitor cells (HPCs), the replicative senescence phenotype and cellular heterogeneity. In this review, we provide an overview of nearly all protocols used for isolating and culturing mBMSCs with the aim of clarifying the most important guidelines for culturing highly purified mBMSC populations retaining in vitro and in vivo differentiation potential.

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In utero xenotransplantation of mice bone marrow-derived stromal/stem cells into fetal rat liver: A preliminary study

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v13i9.7665 ◽

2020 ◽

Author(s):

Maryam Kasraeian ◽

Elahe Ghasemi ◽

Mehdi Dianatpour ◽

Nader Tanideh ◽

Iman Razeghian Jahromi ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Liver Tissue ◽

Fluorescent Protein ◽

In Utero ◽

Mouse Bone Marrow ◽

Rat Fetus ◽

Adult Mice ◽

Infant Rats ◽

Stromal Stem Cells

Background: Animals can play an important role in preparing tissues for human through the development of xenotransplantation protocols. The most common problem with liver transplantation like any other organ transplantation is organ supply shortage. Objective: To evaluate the in utero xenotransplantation of mouse bone marrow-derived stromal/stem cells (BMSCs) to the liver of rat fetus to produce mouse liver tissue. Materials and Methods: BMSCs were isolated and confirmed from enhanced green fluorescent protein (eGFP)-genetic labeled mice. Using a microinjection protocol, mice BMSCs were injected into the liver of rat fetuses in utero on day 14 of pregnancy. After birth, livers were collected and the presence of mice eGFP-positive cells in rat livers was evaluated through polymerase chain reaction. Results: The eGFP mRNA was detected in the liver of injected infant rats. BMSCs of adult mice were capable to remain functional probably as hepatocyte-like cells in liver of infant rats after in utero xenotransplantation. Conclusion: BMSCs have the potential for intrauterine xenotransplantation for the treatment of liver dysfunction before birth. This method can also be used for xenoproduction of liver tissue for transplantation. Key words: Xenotransplantation, Liver, Bone marrow, Stromal/stem cell, Murine.

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