scholarly journals Effects of Carthamus Tinctorius Extract on Adipogenic Differentiation of Mouse Bone Marrow-Derived Mesenchymal Stromal Stem Cells

2017 ◽  
Vol 38 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Sung-ryul Yu ◽  
Seon-mi Shin
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adipogenic Differentiation ◽  
Carthamus Tinctorius ◽  
Mouse Bone Marrow ◽  
Stromal Stem Cells

Recent approaches for isolating and culturing mouse bone marrow-derived mesenchymal stromal stem cells

2020 ◽  
Vol 15 ◽  
Author(s):  
Basem M. Abdallah ◽  
Hany M. Khattab
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Molecular Mechanisms ◽  
Replicative Senescence ◽  
Cellular Heterogeneity ◽  
High Yield ◽  
Mouse Strains ◽  
Mouse Bone Marrow ◽  
Differentiation Potential ◽  
Stromal Stem Cells

: The isolation and culture of murine bone marrow-derived mesenchymal stromal stem cells (mBMSCs) have attracted great interest in terms of the pre-clinical applications of stem cells in tissue engineering and regenerative medicine. In addition, culturing mBMSCs is important for studying the molecular mechanisms of bone remodelling using relevant transgenic mice. Several factors have created challenges in the isolation and high-yield expansion of homogenous mBMSCs; these factors include low frequencies of bone marrow-derived mesenchymal stromal stem cells (BMSCs) in bone marrow, variation among inbred mouse strains, contamination with haematopoietic progenitor cells (HPCs), the replicative senescence phenotype and cellular heterogeneity. In this review, we provide an overview of nearly all protocols used for isolating and culturing mBMSCs with the aim of clarifying the most important guidelines for culturing highly purified mBMSC populations retaining in vitro and in vivo differentiation potential.

scholarly journals In utero xenotransplantation of mice bone marrow-derived stromal/stem cells into fetal rat liver: A preliminary study

2020 ◽  
Author(s):  
Maryam Kasraeian ◽  
Elahe Ghasemi ◽  
Mehdi Dianatpour ◽  
Nader Tanideh ◽  
Iman Razeghian Jahromi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Liver Tissue ◽  
Fluorescent Protein ◽  
In Utero ◽  
Mouse Bone Marrow ◽  
Rat Fetus ◽  
Adult Mice ◽  
Infant Rats ◽  
Stromal Stem Cells

Background: Animals can play an important role in preparing tissues for human through the development of xenotransplantation protocols. The most common problem with liver transplantation like any other organ transplantation is organ supply shortage. Objective: To evaluate the in utero xenotransplantation of mouse bone marrow-derived stromal/stem cells (BMSCs) to the liver of rat fetus to produce mouse liver tissue. Materials and Methods: BMSCs were isolated and confirmed from enhanced green fluorescent protein (eGFP)-genetic labeled mice. Using a microinjection protocol, mice BMSCs were injected into the liver of rat fetuses in utero on day 14 of pregnancy. After birth, livers were collected and the presence of mice eGFP-positive cells in rat livers was evaluated through polymerase chain reaction. Results: The eGFP mRNA was detected in the liver of injected infant rats. BMSCs of adult mice were capable to remain functional probably as hepatocyte-like cells in liver of infant rats after in utero xenotransplantation. Conclusion: BMSCs have the potential for intrauterine xenotransplantation for the treatment of liver dysfunction before birth. This method can also be used for xenoproduction of liver tissue for transplantation. Key words: Xenotransplantation, Liver, Bone marrow, Stromal/stem cell, Murine.

scholarly journals CRISPR/Cas9-mediated reversibly immortalized mouse bone marrow stromal stem cells (BMSCs) retain multipotent features of mesenchymal stem cells (MSCs)

Oncotarget ◽  
2017 ◽  
Vol 8 (67) ◽  
pp. 111847-111865 ◽  
Author(s):  
Xue Hu ◽  
Li Li ◽  
Xinyi Yu ◽  
Ruyi Zhang ◽  
Shujuan Yan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Mouse Bone Marrow ◽  
Stromal Stem Cells

MicroRNA-338-3p Regulates Osteogenic Differentiation of Mouse Bone Marrow Stromal Stem Cells by Targeting Runx2 and Fgfr2

2014 ◽  
Vol 229 (10) ◽  
pp. 1494-1502 ◽  
Author(s):  
Huan Liu ◽  
Qin Sun ◽  
Chunyan Wan ◽  
Lu Li ◽  
Lu Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Mouse Bone Marrow ◽  
Stromal Stem Cells

scholarly journals Comparisons of Mouse Mesenchymal Stem Cells in Primary Adherent Culture of Compact Bone Fragments and Whole Bone Marrow

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Yiting Cai ◽  
Tianshu Liu ◽  
Fang Fang ◽  
Chengliang Xiong ◽  
Shiliang Shen
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipogenic Differentiation ◽  
Compact Bone ◽  
Mouse Bone Marrow ◽  
Alternative Source ◽  
Proliferation Capacity ◽  
Bone Fragments ◽  
Adherent Culture

The purification of mouse bone marrow mesenchymal stem cells (BMSCs) by using the standard method of whole bone marrow adherence to plastic still remains ineffective. An increasing number of studies have indicated compact bone as an alternative source of BMSCs. We isolated BMSCs from cultured compact bone fragments and investigated the proliferative capacity, surface immunophenotypes, and osteogenic and adipogenic differentiations of the cells after the first trypsinization. The fragment culture was based on the fact that BMSCs were assembled in compact bones. Thus, the procedure included flushing bone marrow out of bone cavity and culturing the fragments without any collagenase digestion. The cell yield from cultured fragments was slightly less than that from cultured bone marrow using the same bone quantity. However, the trypsinized cells from cultured fragments exhibited significantly higher proliferation and were accompanied with more CD90 and CD44 expressions and less CD45 expression. The osteogenic and adipogenic differentiation capacity of cells from cultured fragments were better than those of cells from bone marrow. The directly adherent culture of compact bone is suitable for mouse BMSC isolation, and more BMSCs with potentially improved proliferation capacity can be obtained in the primary culture.

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