Correlation of Radiation Response of Cervical Cancer Stem Cells with Their Initial Number before Treatment and Molecular Genetic Features of Papillomavirus Infection

2020 ◽  
Vol 170 (2) ◽  
pp. 241-245
Author(s):  
I. A. Zamulaeva ◽  
E. I. Selivanova ◽  
V. I. Kiseleva ◽  
O. N. Matchuk ◽  
L. I. Krikunova ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Molecular Genetic ◽  
Radiation Response ◽  
Initial Number ◽  
Papillomavirus Infection ◽  
Genetic Features

scholarly journals Radiation Response of Cervical Cancer Stem Cells Is Associated with Pretreatment Proportion of These Cells and Physical Status of HPV DNA

2021 ◽  
Vol 22 (3) ◽  
pp. 1445
Author(s):  
Irina Zamulaeva ◽  
Elena Selivanova ◽  
Olga Matchuk ◽  
Valentina Kiseleva ◽  
Liana Mkrtchyan ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Hpv Infection ◽  
Lymph Node Involvement ◽  
Radiation Response ◽  
Individual Response ◽  
Morphological Parameters ◽  
Physical Status ◽  
Hpv Dna

Radio- and chemoresistance of cancer stem cells (CSCs) is considered as one of the possible causes of adverse results of chemoradiotherapy for various malignancies, including cervical cancer. However, little is known about quantitative changes in the CSC subpopulation in the course of treatment and mechanisms for individual response of CSCs to therapy. The purpose of the study was to evaluate the association of radiation response of cervical CSCs with clinical and morphological parameters of disease and features of human papillomavirus (HPV) infection. The proportion of CD44+CD24low CSCs was determined by flow cytometry in cervical scrapings from 55 patients with squamous cell carcinoma of uterine cervix before treatment and after fractionated irradiation at a total dose of 10 Gy. Real-time PCR assay was used to evaluate molecular parameters of HPV DNA. Post-radiation increase in the CSC proportion was found in 47.3% of patients. Clinical and morphological parameters (stage, status of lymph node involvement, and histological type) were not significantly correlated with radiation changes in the CSC proportion. Single- and multifactor analyses revealed two independent indicators affecting the radiation response of CSCs: initial proportion of CSCs and physical status of HPV DNA (R = 0.86, p = 0.001 for the multiple regression model in the whole).

scholarly journals Production of interleukin-4 in CD133+ cervical cancer stem cells promotes resistance to apoptosis and initiates tumor growth

2016 ◽  
Vol 14 (4) ◽  
pp. 4008-4008
Author(s):  
Chun-Tao Liu ◽  
Ying Xin ◽  
Chun-Yan Tong ◽  
Bing Li ◽  
Hong-Li Bao ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Tumor Growth ◽  
Interleukin 4

scholarly journals LGR6 activates the Wnt/β-catenin signaling pathway and forms a β-catenin/TCF7L2/LGR6 feedback loop in LGR6high cervical cancer stem cells

Oncogene ◽  
2021 ◽  
Author(s):  
Qian Feng ◽  
Shan Li ◽  
Hong-Mei Ma ◽  
Wen-Ting Yang ◽  
Peng-Sheng Zheng
Keyword(s):  
Cervical Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Wnt Signaling ◽  
Signaling Pathway ◽  
Western Blotting ◽  
Positive Feedback ◽  
Feedback Loop ◽  
Stem Cell Marker ◽  
Positive Feedback Loop

AbstractThe leucine-rich repeat-containing G-protein-coupled receptor 6 (LGR6) is considered to be a stem cell marker in many normal tissues and promotes tissue development, regeneration, and repair. LGR6 is also related to the initiation and progression of some malignant tumors. However, the role of LGR6 in cervical cancer has not been reported. Here, immunohistochemistry and western blotting showed that LGR6 was significantly upregulated in cervical cancer, compared with the normal cervix. By analyzing The Cancer Genome Atlas database, LGR6 was found to be correlated with a poor prognosis of cervical cancer. Then, a small population of LGR6high cells isolated by using the fluorescence-activated cell sorting exhibited enhanced properties of cancer stem cells including self-renewal, differentiation, and tumorigenicity. Moreover, RNA sequencing revealed that LGR6 was correlated with the Wnt signaling pathway and TOP/FOP, reverse transcription-PCR, and western blotting further proved that LGR6 could activate the Wnt/β-catenin signaling pathway. Interestingly, LGR6 upregulated the expression of TCF7L2 by activating the Wnt/β-catenin pathway. Then, TCF7L2 combining with β-catenin in the nucleus enhanced LGR6 transcription by binding the promoter of LGR6, which further activated the Wnt signaling to form a positive feedback loop. Thus, our study demonstrated that LGR6 activated a novel β-catenin/TCF7L2/LGR6-positive feedback loop in LGR6high cervical cancer stem cells (CSCs), which provided a new therapeutic strategy for targeting cervical CSCs to improve the prognosis of cervical cancer patients.

Abstract 4079: Role of HPVE6 oncoprotein in the maintenance of cervical cancer stem cells

2015 ◽  
Author(s):  
Bhudev C. Das ◽  
Abhishek Tyagi ◽  
Kanchan Vishnoi ◽  
Sutapa Mahata ◽  
Gaurav Verma ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells

scholarly journals Isolation and characterization of cancer stem cells from cervical cancer HeLa cells

2012 ◽  
Vol 64 (4) ◽  
pp. 477-484 ◽  
Author(s):  
Song-Ling Zhang ◽  
Yi-Shu Wang ◽  
Tong Zhou ◽  
Xiao-Wei Yu ◽  
Zhen-Tong Wei ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Hela Cells ◽  
Isolation And Characterization

scholarly journals A1E reduces stemness and self-renewal in HPV 16-positive cervical cancer stem cells

2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Taeho Kwon ◽  
Yesol Bak ◽  
Sun-Young Ham ◽  
Dae-Yeul Yu ◽  
Do-Young Yoon
Keyword(s):  
Cervical Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Self Renewal ◽  
Hpv 16

scholarly journals TAB2 Promotes the Stemness and Biological Functions of Cervical Squamous Cell Carcinoma Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Yijia Zhou ◽  
Yuandong Liao ◽  
Chunyu Zhang ◽  
Junxiu Liu ◽  
Wei Wang ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cancer Cells ◽  
Cancer Progression ◽  
Cell Function ◽  
Chemotherapy Resistance ◽  
Cervical Squamous Cell Carcinoma ◽  
Published Data ◽  
Cell Functions

Cancer stem cells are a key population participating in the promotion of the cervical cancer progression through interacting with cancer cells. Existing studies have preliminary revealed that cervical cancer stem cells contribute to tumor recurrence and chemotherapy resistance. However, the specific mechanisms involved in regulating cell functions remain largely unknown. Here, we analyzed published data from public databases and our global transcriptome data, thus identifying cancer-related signaling pathways and molecules. According to our findings, upregulated TAB2 was correlated to stem cell-like properties of cervical cancer. Immunohistochemistry staining of TAB2 in normal and cervical cancer tissues was performed. The cell function experiments demonstrated that knockdown of TAB2 reduced the stemness of cervical cancer cells and, importantly, prevented cervical cancer progression. Collectively, the therapeutic scheme targeting TAB2 may provide an option for overcoming tumor relapse and chemoresistance of cervical cancer via obstructing stemness maintenance.

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