Resistance to Antibiotics in Plankton and Biofilm Cultures of Pseudomonas aeruginosa Clinical Strains

Introduction/Aim. Pseudomonas aeruginosa (P. aeruginosa) is the most common cause of wound infections, following the disruption of the skin or mucous membranes integrity. The aim of this study was to analyze of the presence P. aeruginosa in wound swabs, antibiotics susceptibility testing, determination of the minimum inhibitory concentrations (MICs) of antibiotics, testing of the metallo-?-lactamases (MBLs) production, isolates serotyping and analysis of the most common serotypes resistance. Methods. A total of 90 outpatients and 55 intpatients wound swabs were cultivated. Wound swabs were taken from the patients with wound infections symptoms. Antibiotics susceptibility testing was performed to: meropenem, imipenem, piperacillin-tazobactam, ceftazidime, cefepime, amikacin, gentamicin, netilmicin, ofloxacin, ciprofloxacin and colistin (HiMedia). Polyvalent and monovalent antisera for agglutination (Biorad) were used in P. aeruginosa agglutination. Results. P. aeruginosa was isolated from 36.55% wound swabs (36.66% of the inpatients wounds and 36.36% of the outpatients). The analyzed isolates showed the highest degree of sensitivity to colistin (100%) and meropenem (93.44%) and the lowest to cefepime (19.54%). The majority of the inpatients isolates had 12 ?g/mL (28.57%) MIC for piperacillin-tazobactam and 16 ?g/mL (28.57%) for the outpatients. The most common MICs for ciprofloxacin were 0.19 ?g/mL (31.81%) for the nosocomial isolates, and 0.25 ?g/mL (28.57%) for the outpatients? ones. The most common MICs for amikacin of the nosocomial isolates were 6 ?g/ml (40.9%), and for the outpatients ones 4 ?g/mL (33.33%). Five (9.43%) isolates produced MBLs. The most common serotypes were P11 (22.64%), P6 (15.09%) and P1 (11.32%). Conclusion. Neither the increased presence of P. aeruginosa was noticed in wounds swabs, nor the antibiotic resistance in the nosocomial isolates compared to those from outpatients. The analyzed isolates had the higest sensitivity to colistin and meropenem, and the lowest to cefepime.

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Pyocine Typing of Clinical Strains of Pseudomonas aeruginosa

Applied Microbiology ◽

10.1128/am.17.2.293-296.1969 ◽

1969 ◽

Vol 17 (2) ◽

pp. 293-296

Author(s):

Ronald J. Zabransky ◽

Fred E. Day

Keyword(s):

Pseudomonas Aeruginosa ◽

Clinical Strains

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Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production

Srpski arhiv za celokupno lekarstvo ◽

10.2298/sarh180727102p ◽

2020 ◽

Vol 148 (3-4) ◽

pp. 196-202

Author(s):

Snjezana Petrovic ◽

Jasmina Basic ◽

Zoran Mandinic ◽

Dragana Bozic ◽

Marina Milenkovic ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Biofilm Formation ◽

Laboratory Strain ◽

Inhibitory Effect ◽

Negative Control ◽

Clinical Strains ◽

Control Value ◽

Essential Components ◽

Pyocyanin Production

Introduction/Objective. Biofilm and pyocyanin production are essential components of Pseudomonas aeruginosa virulence and antibiotic resistance. Our objective was to examine inhibitory effect of synthetized propafenone derivatives 3-(2-Fluorophenyl)- 1-(2- (2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5OF) and3-(2- Trifluoromethyl-phenyl)-1-(2-(2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5CF3) on biofilm and pyocyanin in Pseudomonas aeruginosa clinical strains. Methods. Effects were tested on nine clinical isolates and one control laboratory strain of P. aeruginosa. In vitro analysis of biofilm growing was performed by incubating bacteria (0.5 McFarland) with 5OF and 5CF3 (500?31.2 ?g/ml) and measuring optical density (OD) at 570 nm. Bacteria in medium without compounds were positive control. Blank medium (an uninoculated medium without test compounds) was used as negative control. Pyocyanin production was estimated by OD at 520 nm, after bacteria incubated with 5CF3 and 5OF (250 and 500 ?g/ml), treated with chloroform, and chloroform layer mixed with HCl. Results. A total of 500 ?g/ml of 5OF and 5CF3 completely inhibited biofilm formation in 10/10 and 4/10 strains, respectively. A total of 250 ?g/ml of 5OF and 5CF3 strongly inhibited biofilm formation in 7/10 strains, while inhibition with 125 ?g/ml of 5OF and 5CF3 was moderate. Lower concentrations had almost no effect on biofilm production. Pyocyanin production was reduced to less than 40% of the control value in 6/9, and less than 50% of the control in 7/9 strains with 500 ?g/ml of 5OF and 5CF3, respectively. At 250 ?g/ml 5OF and 5CF3, most strains had pyocyanin production above 50% of the control value. Conclusion. Synthetized propafenone derivatives, 5OF and 5CF3, inhibited biofilms and pyocyanin production of Pseudomonas aeruginosa clinical strains. Presented results suggest that propafenone derivatives are potential lead-compounds for synthesis of novel antipseudomonal drugs.

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Molecular detection and sequencing of carbapenemase-encoding genes in clinical strains of Pseudomonas aeruginosa

Applied Microbiology open access ◽

10.4172/2471-9315-c3-016 ◽

2018 ◽

Vol 04 ◽

Author(s):

Ayman Kamal El Essawy

Keyword(s):

Pseudomonas Aeruginosa ◽

Molecular Detection ◽

Clinical Strains ◽

Encoding Genes

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Draft Genome Sequences of Four Pseudomonas aeruginosa Clinical Strains with Various Biofilm Phenotypes

Microbiology Resource Announcements ◽

10.1128/mra.01286-19 ◽

2020 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Amine M. Boukerb ◽

Marjolaine Simon ◽

Erwan Pernet ◽

Albane Jouault ◽

Emilie Portier ◽

...

Keyword(s):

Cystic Fibrosis ◽

Pseudomonas Aeruginosa ◽

Genome Size ◽

Draft Genome ◽

Genome Sequences ◽

Content Type ◽

Clinical Strains

Biofilms produced by Pseudomonas aeruginosa present a serious threat to cystic fibrosis patients. Here, we report the draft genome sequences of four cystic fibrosis isolates displaying various mucoid and biofilm phenotypes. The estimated average genome size was about 6,255,986 ± 50,202 bp with a mean G+C content of 66.52 ± 0.06%.

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