Deleterious effects of Bacillus thuringiensis on biological parameters of Habrobracon hebetor parasitizing Helicoverpa armigera

BioControl ◽  
2013 ◽  
Vol 59 (1) ◽  
pp. 89-98 ◽  
Author(s):  
Amin Sedaratian ◽  
Yaghoub Fathipour ◽  
Reza Talaei-Hassanloui
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Armigera ◽  
Biological Parameters ◽  
Habrobracon Hebetor ◽  
Helicoverpa Armígera

scholarly journals Behavioral and Developmental Responses of Habrobracon hebetor (Hymenoptera: Braconidae) to Larvae of Helicoverpa armigera (Lepidoptera: Noctuidae) Inoculated With Various Concentrations of Bacillus thuringiensis var. kurstaki (Bacillales: Bacillacae)

2020 ◽  
Vol 20 (6) ◽  
Author(s):  
Rahim Allahyari ◽  
Shahram Aramideh ◽  
J P Michaud ◽  
Mohammad Hassan Safaralizadeh ◽  
Mohammad Reza Rezapanah
Keyword(s):  
Biological Control ◽  
Bacillus Thuringiensis ◽  
Helicoverpa Armigera ◽  
Choice Test ◽  
Habrobracon Hebetor ◽  
Combined Application ◽  
Joint Application ◽  
Immature Development ◽  
Helicoverpa Armígera ◽  
Parasitoid Development

Abstract Bacillus thuringiensis Berliner subsp. kurstaki (Btk) and Habrobracon hebetor Say are both biological control agents of Helicoverpa armigera Hubner. The present study evaluated their compatibility for combined application against this pest by examining the acceptability of Btk-inoculated hosts for H. hebetor females and testing for negative life-history impacts on developing progeny. Second-instar H. armigera larvae fed for 72 h on potted chickpea plants treated with three concentrations of Btk (LC15, LC35, and LC70) and were then used in bioassays of parasitoid development and parasitism behavior. Survival of parasitoids was significantly reduced, and immature development prolonged, on hosts fed chickpea plants treated with LC35 and LC70  Btk, but not on plants treated with LC15  Btk. Parasitoids failed to discriminate against hosts treated with LC15 or LC35  Btk in choice tests, but attacked fewer hosts treated with LC70  Btk, paralyzing and parasitizing more healthy hosts, and laying more eggs on them. In contrast, a no-choice test revealed that more hosts treated with LC35 and LC70  Btk were paralyzed compared with control or LC15-treated hosts, but the numbers of hosts parasitized and eggs laid did not vary among Btk treatments. Thus, females required an experience with healthy hosts, as they had in the choice test, to discriminate against diseased ones. We conclude that H. hebetor and Btk are compatible for joint application against H. armigera, which could potentially improve biological control of this pest.

scholarly journals Bacillus thuringiensis Bel Protein Enhances the Toxicity of Cry1Ac Protein to Helicoverpa armigera Larvae by Degrading Insect Intestinal Mucin

2009 ◽  
Vol 75 (16) ◽  
pp. 5237-5243 ◽  
Author(s):  
Shangling Fang ◽  
Li Wang ◽  
Wei Guo ◽  
Xia Zhang ◽  
Donghai Peng ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Armigera ◽  
Trichoplusia Ni ◽  
Knockout Mutant ◽  
Gene Encoding ◽  
In Vitro Degradation ◽  
Intestinal Mucin ◽  
Helicoverpa Armígera ◽  
Cry1ac Protein

ABSTRACT Bacillus thuringiensis has been used as a bioinsecticide to control agricultural insects. Bacillus cereus group genomes were found to have a Bacillus enhancin-like (bel) gene, encoding a peptide with 20 to 30% identity to viral enhancin protein, which can enhance viral infection by degradation of the peritrophic matrix (PM) of the insect midgut. In this study, the bel gene was found to have an activity similar to that of the viral enhancin gene. A bel knockout mutant was constructed by using a plasmid-free B. thuringiensis derivative, BMB171. The 50% lethal concentrations of this mutant plus the cry1Ac insecticidal protein gene were about 5.8-fold higher than those of the BMB171 strain. When purified Bel was mixed with the Cry1Ac protein and fed to Helicoverpa armigera larvae, 3 μg/ml Cry1Ac alone induced 34.2% mortality. Meanwhile, the mortality rate rose to 74.4% when the same amount of Cry1Ac was mixed with 0.8 μg/ml of Bel. Microscopic observation showed a significant disruption detected on the midgut PM of H. armigera larvae after they were fed Bel. In vitro degradation assays showed that Bel digested the intestinal mucin (IIM) of Trichoplusia ni and H. armigera larvae to various degrading products, similar to findings for viral enhancin. These results imply Bel toxicity enhancement depends on the destruction of midgut PM and IIM, similar to the case with viral enhancin. This discovery showed that Bel has the potential to enhance insecticidal activity of B. thuringiensis-based biopesticides and transgenic crops.

scholarly journals Biological parameters of three Trichogramma pretiosum strains (Riley, 1879) (Hymenoptera: Trichogrammatidae) on eggs Helicoverpa armigera (Hübner, 1805) (Lepidoptera: Noctuidae)

2017 ◽  
Vol 39 (3) ◽  
pp. 349 ◽  
Author(s):  
Gabriel dos Santos Carvalho ◽  
Luciana Barbosa Silva ◽  
Maisa Sousa Veras ◽  
Eliane Carneiro Bueno dos Santos ◽  
Mayra Layra dos Santos Almeida ◽  
...  
Keyword(s):  
Helicoverpa Armigera ◽  
Biological Parameters ◽  
Trichogramma Pretiosum ◽  
Helicoverpa Armígera ◽  
Lepidoptera Noctuidae

scholarly journals Vip3A is responsible for the potency of Bacillus thuringiensis 9816C culture supernatant against Helicoverpa armigera and Spodoptera exigua

2006 ◽  
Vol 52 (2) ◽  
pp. 83-89 ◽  
Author(s):  
Jun Cai ◽  
Liang Xiao ◽  
Bing Yan ◽  
Guan Bin ◽  
Yuehua Chen ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Armigera ◽  
Spodoptera Exigua ◽  
Culture Supernatant ◽  
Helicoverpa Armígera

scholarly journals POTENCIAL INSETICIDA DO EXTRATO ETANÓLICO DE Anadenanthera macrocarpa (BENTH.) EM LEPIDÓPTEROS-PRAGA

Nativa ◽  
2019 ◽  
Vol 7 (6) ◽  
pp. 668
Author(s):  
Glauciany Soares Lopes ◽  
Luciana Barboza Silva ◽  
Eliane Carneiro ◽  
Manoel Lopes da Silva Filho ◽  
João Sammy Nery de Souza ◽  
...  
Keyword(s):  
Helicoverpa Armigera ◽  
Spodoptera Frugiperda ◽  
Ethanolic Extract ◽  
Chemical Compounds ◽  
Biological Parameters ◽  
Biological Cycle ◽  
Period Increase ◽  
Lepidopteran Species ◽  
Helicoverpa Armígera ◽  
Efficient Alternative

O complexo de lagartas desfolhadoras - Spodoptera cosmioides, Spodoptera frugiperda e Helicoverpa armigera, são responsáveis por uma considerável redução na produção de grãos. A aplicação de extratos botânicos consiste em uma alternativa viável e eficiente de controle, além de ser menos nociva ao meio ambiente e a saúde do homem. O objetivo foi avaliar o potencial inseticida do extrato bruto de folhas e casca de Anadenanthera macrocarpa, sobre as lagartas de S. frugiperda, S. cosmioides, H. armigera. Os insetos foram mantidos em dieta artificial em condições controladas. Foi avaliada a ação toxica dos extratos por aplicação tópica. Primeiramente foi estimada a concentração letal e em seguida foram determinados os parâmetros biológicos das três espécies de lepidópteros. Os extratos etanólicos de folhas e casca de A. macrocarpa foram eficientes no controle de H. armigera, S. frugiperda e S. cosmioides reduzindo a sobrevivência em 75, 60 e 50%, respectivamente, além de alterações nos parâmetros biológicos como, aumento do período larval, redução de peso das larvas e pupas. Os compostos químicos presentes nos extratos etanólicos de folhas e casca de A. macrocarpa, além de causar mortalidade direta dos insetos estudados, alteram o ciclo biológico de S. frugiperda, S. cosmoides e H. armigera.Palavras-chave: Angico-preto; Spodoptera cosmioides; Spodoptera frugiperda; Helicoverpa armigera; bioinseticidas. POTENTIAL INSECTICIDE OF THE ETHANOLIC EXTRACT OF Anadenanthera macrocarpa (BENTH.) IN LEPIDOPTERAN-PESTS ABSTRACT: The leafless worm complex - Spodoptera cosmioides, Spodoptera frugiperda and Helicoverpa armigera - are responsible for a considerable reduction in grain production. The application of botanical extracts is a viable and efficient alternative of control, besides being less harmful to the environment and human health. The objective was to evaluate the insecticidal potential of the raw extract of leaves and bark of Anadenanthera macrocarpa, on S. frugiperda, S. cosmioides, H. armigera. The insects were kept on artificial diet under controlled conditions. The toxic action of the extracts was evaluated by topical application. First the lethal concentration was estimated and then the biological parameters of the three lepidopteran species were determined. The extracts of leaves and bark of A. macrocarpa were efficient in the control of H. armigera, S. frugiperda and S.a cosmioides, reducing survival in 75, 60 and 50%, respectively, as well as changes in biological parameters such as larval period increase, weight reduction of larvae and pupae. The chemical compounds present in the leaves and bark extracts of A. macrocarpa, besides causing direct mortality of the studied insects, alter the biological cycle of S. frugiperda, S. cosmoides and H. armigera.Keywords: Angico-black; Spodoptera cosmioides; Spodoptera frugiperda; Helicoverpa armígera; bioinsecticides.

scholarly journals Expression of Cry2Aa, a Bacillus thuringiensis insecticidal protein in transgenic pigeon pea confers resistance to gram pod borer, Helicoverpa armigera

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Shweta Singh ◽  
Nikhil Ram Kumar ◽  
R. Maniraj ◽  
R. Lakshmikanth ◽  
K. Y. S. Rao ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Armigera ◽  
Pigeon Pea ◽  
Insecticidal Protein ◽  
Pod Borer ◽  
Helicoverpa Armígera
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