Rapeseed Transformation with aroA Bacterial Gene Containing P101S Mutation Confers Glyphosate Resistance

2021 ◽  
Author(s):  
Amir Roeintan ◽  
Seyed Mehdi Safavi ◽  
Danial Kahrizi
Keyword(s):  
Glyphosate Resistance ◽  
Bacterial Gene

EXPRESSION IN PLANTS OF A BACTERIAL GENE CODING FOR GLYPHOSATE RESISTANCE

1985 ◽  
pp. 329-338
Author(s):  
Luca Comai ◽  
Daniel Facciotti ◽  
David M. Stalker ◽  
Gregory A. Thompson ◽  
William R. Hiatt
Keyword(s):  
Glyphosate Resistance ◽  
Bacterial Gene ◽  
Gene Coding

scholarly journals Evolutionary epidemiology predicts the emergence of glyphosate resistance in a major agricultural weed

2019 ◽  
Vol 223 (3) ◽  
pp. 1584-1594 ◽  
Author(s):  
David Comont ◽  
Helen Hicks ◽  
Laura Crook ◽  
Richard Hull ◽  
Elise Cocciantelli ◽  
...  
Keyword(s):  
Glyphosate Resistance ◽  
Evolutionary Epidemiology ◽  
Agricultural Weed

Gut bacterial gene changes following pegaspargase treatment in pediatric patients with acute lymphoblastic leukemia

2021 ◽  
pp. 1-12
Author(s):  
Katherine A. Dunn ◽  
Zara Forbrigger ◽  
Jessica Connors ◽  
Mushfiqur Rahman ◽  
Alejandro Cohen ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Pediatric Patients ◽  
Lymphoblastic Leukemia ◽  
Bacterial Gene

scholarly journals Bacterial Resistance to Antisense Peptide Phosphorodiamidate Morpholino Oligomers

2012 ◽  
Vol 56 (12) ◽  
pp. 6147-6153 ◽  
Author(s):  
Susan E. Puckett ◽  
Kaleb A. Reese ◽  
Georgi M. Mitev ◽  
Valerie Mullen ◽  
Rudd C. Johnson ◽  
...  
Keyword(s):  
Bacterial Resistance ◽  
Acyl Carrier Protein ◽  
Essential Gene ◽  
Resistant Mutant ◽  
Resistant Isolate ◽  
Carrier Protein ◽  
Bacterial Gene ◽  
Content Type ◽  
Bacterial Gene Expression ◽  
Phosphorodiamidate Morpholino Oligomers

ABSTRACTPeptide phosphorodiamidate morpholino oligomers (PPMOs) are synthetic DNA mimics that bind cRNA and inhibit bacterial gene expression. The PPMO (RFF)3RXB-AcpP (where R is arginine, F, phenylalanine, X is 6-aminohexanoic acid, B is β-alanine, and AcpP is acyl carrier protein) is complementary to 11 bases of the essential geneacpP(which encodes acyl carrier protein). The MIC of (RFF)3RXB-AcpP was 2.5 μM (14 μg/ml) inEscherichia coliW3110. The rate of spontaneous resistance ofE. colito (RFF)3RXB-AcpP was 4 × 10−7mutations/cell division. A spontaneous (RFF)3RXB-AcpP-resistant mutant (PR200.1) was isolated. The MIC of (RFF)3RXB-AcpP was 40 μM (224 μg/ml) for PR200.1. The MICs of standard antibiotics for PR200.1 and W3110 were identical. The sequence ofacpPwas identical in PR200.1 and W3110. PR200.1 was also resistant to other PPMOs conjugated to (RFF)3RXB or peptides with a similar composition or pattern of cationic and nonpolar residues. Genomic sequencing of PR200.1 identified a mutation insbmA, which encodes an active transport protein. In separate experiments, a (RFF)3RXB-AcpP-resistant isolate (RR3) was selected from a transposome library, and the insertion was mapped tosbmA. Genetic complementation of PR200.1 or RR3 withsbmArestored susceptibility to (RFF)3RXB-AcpP. Deletion ofsbmAcaused resistance to (RFF)3RXB-AcpP. We conclude that resistance to (RFF)3RXB-AcpP was linked to the peptide and not the phosphorodiamidate morpholino oligomer, dependent on the composition or repeating pattern of amino acids, and caused by mutations insbmA. The data further suggest that (RFF)3R-XB PPMOs may be transported across the plasma membrane by SbmA.

scholarly journals Gene-Directed Enzyme Prodrug Therapy by Dendrimer-Like Mesoporous Silica Nanoparticles against Tumor Cells

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1298
Author(s):  
Vicente Candela-Noguera ◽  
Gema Vivo-Llorca ◽  
Borja Díaz de Greñu ◽  
María Alfonso ◽  
Elena Aznar ◽  
...  
Keyword(s):  
Cell Death ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Tumor Cells ◽  
Mesoporous Silica Nanoparticles ◽  
Combined Treatment ◽  
Bond Rupture ◽  
Enzyme Prodrug Therapy ◽  
Bacterial Gene ◽  
Reducing Environment

We report herein a gene-directed enzyme prodrug therapy (GDEPT) system using gated mesoporous silica nanoparticles (MSNs) in an attempt to combine the reduction of side effects characteristic of GDEPT with improved pharmacokinetics promoted by gated MSNs. The system consists of the transfection of cancer cells with a plasmid controlled by the cytomegalovirus promoter, which promotes β-galactosidase (β-gal) expression from the bacterial gene lacZ (CMV-lacZ). Moreover, dendrimer-like mesoporous silica nanoparticles (DMSNs) are loaded with the prodrug doxorubicin modified with a galactose unit through a self-immolative group (DOXO-Gal) and modified with a disulfide-containing polyethyleneglycol gatekeeper. Once in tumor cells, the reducing environment induces disulfide bond rupture in the gatekeeper with the subsequent DOXO-Gal delivery, which is enzymatically converted by β-gal into the cytotoxic doxorubicin drug, causing cell death. The combined treatment of the pair enzyme/DMSNs-prodrug are more effective in killing cells than the free prodrug DOXO-Gal alone in cells transfected with β-gal.

scholarly journals Junglerice (Echinochloa colona) Control with Sequential Applications of Glyphosate and Clethodim to Dicamba

2021 ◽  
pp. 1-18
Author(s):  
Clay M. Perkins ◽  
Thomas C. Mueller ◽  
Lawrence E. Steckel
Keyword(s):  
Glyphosate Resistance ◽  
Time Of Application ◽  
Crop Fields ◽  
Row Crop ◽  
Excellent Control ◽  
Sequential Treatments

Abstract Junglerice is becoming more prevalent in Tennessee, Arkansas and Mississippi row crop fields. The evolution of glyphosate-resistant junglerice populations is one reason for the increase. Another possible explanation is that glyphosate and clethodim grass activity is being antagonized by dicamba. This question has led to research to examine if sequential applications alleviate antagonism observed with dicamba plus glyphosate and/or clethodim mixtures and determine if 24 h, 72 h or 168 h sequential treatments of those herbicides can improve junglerice control. Glyphosate + clethodim applications provided >90% junglerice control. The observed levels of antagonism varied by whether the location of the test was in the greenhouse or the field and the timing of applications. In the greenhouse, clethodim + dicamba provided excellent control while in the field the same treatment showed over a 30% reduction in junglerice control compared with clethodim alone. However, control was restored by using a mixture of glyphosate + clethodim without dicamba. The environment at the time of application and relative glyphosate-resistance (GR) level of the junglerice influenced the overall control of these sequential applications. Clethodim applied first followed by dicamba at 72 or 168 h, better control was observed compared with applying dicamba followed by clethodim. Overall, mixing glyphosate + clethodim provided the most complete junglerice control regardless of timing. These data confirm that leaving dicamba out of the spray tank will mitigate herbicide antagonism on junglerice control. These data would also indicate that avoiding dicamba and glyphosate mixtures will also improve the consistency of control with glyphosate-susceptible junglerice.

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