In vitro regeneration of mature Pinus sylvestris buds stored at freezing temperatures

2005 ◽  
Vol 49 (2) ◽  
pp. 281-284 ◽  
Author(s):  
U. Andersone ◽  
G. Ievinsh
Keyword(s):  
Pinus Sylvestris ◽  
In Vitro Regeneration ◽  
Freezing Temperatures

scholarly journals In vitro regeneration of adult Pinus sylvestris L. trees

2010 ◽  
Vol 76 (1) ◽  
pp. 158-162 ◽  
Author(s):  
N. De Diego ◽  
I.A. Montalbán ◽  
P. Moncaleán
Keyword(s):  
Pinus Sylvestris ◽  
In Vitro Regeneration

In vitro regeneration of plantlets of Scots pine (Pinus sylvestris) with mycorrhizal roots from subcultured callus initiated from needle adventitious buds

1994 ◽  
Vol 72 (8) ◽  
pp. 1144-1150 ◽  
Author(s):  
Supriyanto ◽  
R. Rohr
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
In Vitro Regeneration ◽  
Histological Study ◽  
Axillary Buds ◽  
Adventitious Buds ◽  
Meristematic Tissue ◽  
Hebeloma Cylindrosporum ◽  
Half Strength

Plantlets were regenerated from cultures established from Pinus sylvestris (Scots pine) meristematic tissue. Seedling explants were first stimulated to develop axillary buds. Developing axillary buds produced numerous new meristems that gave rise to globular adventitious buds located along the needles on half-strength modified Murashige and Skoog medium supplemented with coconut milk and 6-benzylaminopurine. A histological study showed that these new buds originated from dedifferentiated mesophyll and epidermal tissues of the needles. Some of these buds were used for the regeneration of whole plantlets, others were excised and transferred to woody plant medium, on which calli developed at the bases of the microcuttings These calli were organogenic when subcultured on a hormone-free medium and initiated a large number of rooted plantlets that showed high potential to multiply themselves indefinitely. This is the first report of regeneration of Scots pine from a subculturable organogenic line. Mycorrhizae were initiated for both types of plantlets with Hebeloma cylindrosporum on a perlite substrate under fully controlled conditions. Mycorrhizae improved the transfer of the plantlets to ex vitro conditions. Key words: organogenesis, mycorrhizae, tissue culture, Pinus sylvestris.

Direct in vitro Regeneration of Arachis Hypogaea L.(Jl-24) and Study of Salt Tolerance

2011 ◽  
Vol 3 (8) ◽  
pp. 52-54 ◽  
Author(s):  
Bhagya Lakshmi Jyothi Kusuma ◽  
◽  
Sharmila Begum S
Keyword(s):  
Salt Tolerance ◽  
Arachis Hypogaea ◽  
In Vitro Regeneration ◽  
Arachis Hypogaea L

In vitro regeneration of watermelon seed segments

2015 ◽  
Vol 4 (8) ◽  
Author(s):  
M. Ameri ◽  
M. Lahouti ◽  
A. Bagheri ◽  
A. Sharifi ◽  
F. Keykha Akhar
Keyword(s):  
In Vitro Regeneration ◽  
Watermelon Seed

In vitro regeneration of Phaseolus vulgaris L. via direct and indirect organogenesis

2021 ◽  
Author(s):  
Yan Yu ◽  
Dajun Liu ◽  
Chang Liu ◽  
Zhishan Yan ◽  
Xiaoxu Yang ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
In Vitro Regeneration ◽  
Phaseolus Vulgaris L ◽  
Indirect Organogenesis

scholarly journals Ovaries of Chrysanthemum Irradiated with High-Energy Photons and High-Energy Electrons Can Regenerate Plants with Novel Traits

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1111
Author(s):  
Natalia Miler ◽  
Iwona Jedrzejczyk ◽  
Seweryn Jakubowski ◽  
Janusz Winiecki
Keyword(s):  
Total Dose ◽  
In Vitro Regeneration ◽  
High Energy ◽  
Mutation Breeding ◽  
Chrysanthemum Morifolium ◽  
Physical Factors ◽  
Strong Impact ◽  
Breeding Method ◽  
High Energy Electrons

Classical mutation breeding using physical factors is a common breeding method for ornamental crops. The aim of our study was to examine the utility of ovaries excised from irradiated inflorescences of Chrysanthemum × morifolium (Ramat.) as explants for breeding purposes. We studied the in vitro regeneration capacity of the ovaries of two chrysanthemum cultivars: ‘Profesor Jerzy’ and ‘Karolina’ preceded by irradiation with high-energy photons (total dose 5, 10 and 15 Gy) and high-energy electrons (total dose 10 Gy). Growth and inflorescence parameters of greenhouse acclimatized regenerants were recorded, and ploidy level was estimated with flow cytometry. The strong impact of genotype on regeneration efficiency was recorded—cultivar ‘Karolina’ produced only 7 viable shoots, while ‘Profesor Jerzy’ produced totally 428 shoots. With an increase of irradiation dose, the regeneration decreased, the least responsive were explants irradiated with 15 Gy high-energy photons and 10 Gy high-energy electrons. Regenerants of ‘Profesor Jerzy’ obtained from these explants possessed shorter stem and flowered later. The highest number of stable, color and shape inflorescence variations were obtained from explants treated with 10 Gy high-energy photons. Variations of inflorescences were predominantly changes of shape—from full to semi-full. New color phenotypes were dark yellow, light yellow and pinkish, among them only the dark yellow phenotype remained stable during second year cultivation. None of the regenerants were haploid. The application of ovaries irradiated within the whole inflorescence of chrysanthemum can be successfully applied in the breeding programs, provided the mother cultivar regenerate in vitro efficiently.

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