In Vitro Regeneration of Plantlets from Cultured Tissues of Scots Pine (Pinus Sylvestris L.)

1985 ◽  
pp. 326-326 ◽  
Author(s):  
Feng H. Huang ◽  
Sonia Tsai
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
In Vitro Regeneration

In vitro regeneration of plantlets of Scots pine (Pinus sylvestris) with mycorrhizal roots from subcultured callus initiated from needle adventitious buds

1994 ◽  
Vol 72 (8) ◽  
pp. 1144-1150 ◽  
Author(s):  
Supriyanto ◽  
R. Rohr
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
In Vitro Regeneration ◽  
Histological Study ◽  
Axillary Buds ◽  
Adventitious Buds ◽  
Meristematic Tissue ◽  
Hebeloma Cylindrosporum ◽  
Half Strength

Plantlets were regenerated from cultures established from Pinus sylvestris (Scots pine) meristematic tissue. Seedling explants were first stimulated to develop axillary buds. Developing axillary buds produced numerous new meristems that gave rise to globular adventitious buds located along the needles on half-strength modified Murashige and Skoog medium supplemented with coconut milk and 6-benzylaminopurine. A histological study showed that these new buds originated from dedifferentiated mesophyll and epidermal tissues of the needles. Some of these buds were used for the regeneration of whole plantlets, others were excised and transferred to woody plant medium, on which calli developed at the bases of the microcuttings These calli were organogenic when subcultured on a hormone-free medium and initiated a large number of rooted plantlets that showed high potential to multiply themselves indefinitely. This is the first report of regeneration of Scots pine from a subculturable organogenic line. Mycorrhizae were initiated for both types of plantlets with Hebeloma cylindrosporum on a perlite substrate under fully controlled conditions. Mycorrhizae improved the transfer of the plantlets to ex vitro conditions. Key words: organogenesis, mycorrhizae, tissue culture, Pinus sylvestris.

Concentrations of terpenes in mycorrhizal roots of Scots pine (Pinus sylvestris L.) seedlings grown in vitro

2002 ◽  
Vol 24 (2) ◽  
pp. 137-143 ◽  
Author(s):  
Anna Napierała-Filipiak ◽  
Antoni Werner ◽  
Marek Mardarowicz ◽  
Jan Gawdzik
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Mycorrhizal Roots

scholarly journals In vitro regeneration of adult Pinus sylvestris L. trees

2010 ◽  
Vol 76 (1) ◽  
pp. 158-162 ◽  
Author(s):  
N. De Diego ◽  
I.A. Montalbán ◽  
P. Moncaleán
Keyword(s):  
Pinus Sylvestris ◽  
In Vitro Regeneration

scholarly journals Extracts of pine bark (Pinus sylvestris) inhibit Cryptosporidium parvum growth in cell culture

2021 ◽  
Author(s):  
Berit Marie Blomstrand ◽  
Heidi Larsen Enemark ◽  
Øivind Øines ◽  
Håvard Steinshamn ◽  
Inga Marie Aasen ◽  
...  
Keyword(s):  
Cell Culture ◽  
Pinus Sylvestris ◽  
Growth Inhibition ◽  
Scots Pine ◽  
Cryptosporidium Parvum ◽  
Dry Matter ◽  
Treatment Options ◽  
Bark Extract ◽  
Dose Dependent

AbstractThe widespread apicomplexan parasite Cryptosporidium parvum is responsible for severe gastrointestinal disease in humans and animals. The treatment options are limited, and the efficacy of available drugs is low. Bark contains condensed tannins (CT), which are bioactive compounds previously shown to inhibit parasite development. Here, we examined the anti-cryptosporidial properties of bark extract of Scots pine (Pinus sylvestris) against C. parvum by means of an in vitro growth inhibition test. We hypothesised that bark extracts would have dose-dependent inhibitory effects on the development of C. parvum in cell culture.Bark extracts from Scots pine extracted with acetone, methanol, and water as solvents were investigated using human colorectal adenocarcinoma cells infected with C. parvum. Oocysts were inoculated onto the cell monolayer and bark extract was added at seven different concentrations. Parasite growth inhibition was quantified by qPCR.The acetone and methanol extracts demonstrated a sigmoid dose-dependent inhibition of C. parvum. The IC50 values were 244.6 and 279.1 µg dry matter extract/mL, and 25.4 and 24.1 µg CT/mL, for acetone and methanol extracts, respectively. The IC50 for both extracts were similar, both with regard to the dry matter concentration of each extract and to CT concentrations.Given the limited treatment options available for Cryptosporidium spp., the evidence generated in our study encourages further investigation into the in vitro and in vivo effects of pine bark extracts against C. parvum.

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