MALTE DE VARIEDADES DE AMARANTO

Objetivou-se obter e avaliar o malte de amaranto, das espécies Amaranthus cruentus, com as variedades BRS Alegria e Verde, e Amaranthus caudatus, com a variedade Inca, produzidas no cerrado mato-grossense. Foi realizado teste de germinação com alternância no fotoperíodo. A qualidade do malte produzido foi avaliada pela análise do poder diastático e o rendimento. Observou-se, pela análise de fotoblastismo, que a variedade verde é fotoblástica neutra e BRS Alegria e Inca são pouco tolerantes à luz constante. O poder diastático, medido pelo índice WK variou em função da temperatura e variedades sendo que a variedade Inca teve maior rendimento e poder diastático (187,4 WK) à temperatura de 25 °C; a temperatura de 25 °C produziu maltes com maior poder diastático, da ordem de 168,4 WK; A variedade BRS Alegria teve menor poder diastático, sendo menos indicada para a produção de malte para fins cervejeiros. Os resultados foram satisfatórios para a produção de bebidas lácteas maltadas, no entanto, para fins cervejeiros, novos ensaios de maltagem devam ser conduzidos, na tentativa de elevar o poder diastático do malte deste pseudocereal para valores próximos do mínimo recomendado para este fim, que é de 220 WK. Palavras-chave: Amaranthus cruentus; Amaranthus caudatus; BRS Alegria; fotoblastismo; poder diastático. Amaranth varieties malt ABSTRACT: The objective of this study was to obtain and evaluate malt amaranth, of the species Amaranthus cruentus, with BRS Alegria and Verde varieties, and Amaranthus caudatus, with Inca variety. Germination test was performed with alternating photoperiod. The quality of the malt produced was evaluated by analyzing the diastatic power and the yield. It was observed, by the analysis of photoblastism, that the Verde variety is photoblastic neutral and BRS Alegria and Inca are poorly tolerant to constant light. Diastatic power, measured by the WK index, varied as a function of temperature and varieties, with the Inca variety having higher yields and diastatic power (187.4 WK) at a temperature of 25 °C; the temperature of 25 °C produced malts with greater diastatic power, in the order of 168.4 WK; The BRS-Alegria variety had lesser diastatic power, being less suitable for the production of malt for brewing purposes. The results were satisfactory for the production of malted dairy beverages, however, for brewing purposes, new malting tests should be conducted, in an attempt to raise the diastatic power of the malt of this pseudocereal to values ​​close to the minimum recommended for this purpose, which is 220 WK. Keywords: Amaranthus cruentus; Amaranthus caudatus; BRS Alegria; photoblastism; diastatic power.

New approaches in the process of crystallization of sucrose in the production of sweets with fondant bodies

Конфеты на основе помадных корпусов пользуются большим спросом у населения. Расширение ассортимента конфет и совершенствование технологии является актуальной проблемой. Актуальными остаются вопросы по расширению ассортимента популярных конфет с сохранением качества не только на производстве, но и при хранении изучение процессов, протекающих при хранении конфет с помадными корпусами, остаются в фокусе научных интересов исследователей. Поставленные задачи наиболее актуальны в производстве конфет на основе помадных корпусов - энергетически неустойчивых систем, имеющих высокую концентрацию сахарозы (более 80 %), простой химический состав, что является причиной быстрой потери потребительских свойств в процессе хранения. Решение указанных проблем возможно за счет введения в рецептуру продуктов переработки амаранта Amaranthus cruentus. Частицы порошка шрота амаранта, являясь влагоудерживающими компонентами, играют также роль компонентов кристаллизации сахарозы в высокопересыщенных растворах. Sweets based on fondant bodies are in great demand among the population. Expanding the assortment of sweets and improving technology is an urgent problem. Questions on expanding the assortment of popular sweets while maintaining quality not only in production, but during storage remain topical. The study of the processes occurring during the storage of sweets with fondant bodies remains in the focus of the scientific interests of researchers. The tasks set are most relevant in the production of sweets based on fondant bodies - energetically unstable systems with a high concentration of sucrose (more than 80 %), a simple chemical composition, which is the reason for the rapid loss of consumer properties during storage. The solution of these problems is possible due to the introduction of Amaranthus cruentus amaranth processed products into the formulation. Particles of amaranth meal powder, being water-retaining components, also play the role of components of crystallization of sucrose in highly supersaturated solutions.

LIPIDS 0F SEEDS MEDICAGO SATIVA, AMARANTHUS CRUENTUS AND AMARANTHUS RETROFLEXUS, GROWING IN GEORGIA

There is studied chemical composition of neutral and polar lipids of the seeds of: Medicago sativa, Amaranlhus cmentus and Amaranthus retroflexus growing in Georgia. The composition of free fatty acids was determined with help GLC and HPLC. There is determined class and fatty acidal composition of them, qualitative and quantitative determination of phospholipids is carried. There is elaborated various medicinal forms from Medicago sativat.

Relationship of Cultivated Grain Amaranth Species and Wild Relative Accessions

Amaranthus is a genus of C4 dicotyledonous herbaceous plants, and three New World species have been domesticated to produce grain crops with light colored seed which are classified as pseudo-cereals rich in protein and minerals. A core collection of grain amaranths and immediate precursor species has been established, representing the closest related species. The goal of this study was to evaluate the genetic diversity in that collection of cultivated and wild species, using competitive allele single nucleotide polymorphism markers. A secondary objective was to determine the relationships among the three cultivated species and non-domesticated Amaranthus, while a third objective was to evaluate the utility of the markers in detecting diversity in the 276 genotypes. The markers were found to be highly variable with an average polymorphism information content of 0.365. All markers were bi-allelic; and the major allele frequency ranged from 0.388 to 0.871. Population structure analysis of the cultigens revealed the presence of two sub populations. Phylogeny confirmed that the two Mesoamerican species, Amaranthus cruentus and Amaranthus hypochondriacus, were related and distant from the South American species Amaranthus caudatus, which in turn was very closely clustered with Amaranthus quitensis, even though this is considered a weedy relative. The first pair of species were likely to have inter-crossed, while the latter two likely exist in a wild-cultivated hybrid state. In conclusion, the results of this SNP study provided insights on amaranth cultivars and their relationship to wild species, the probable domestication events leading to the cultivars, and possible crop breeding or germplasm conservation strategies.

Fusarium casha sp. nov. and F. curculicola sp. nov. in the Fusarium fujikuroi Species Complex Isolated from Amaranthuscruentus and Three Weevil Species in South Africa

Trials are currently being conducted in South Africa to establish Amaranthus cruentus as a new pseudocereal crop. During recent surveys, Fusarium species were associated with weevil damage in A. cruentus fields. Preliminary studies showed that some of these Fusarium species grouped into two distinct clades within the F. fujikuroi species complex. The aim of this study was to characterize these isolates based on the morphology and phylogeny of the translation elongation factor 1α (TEF1α) gene region, ß-tubulin 2 (ßT) gene region and RNA polymerase II subunit (RPB2), and to determine if these isolates are pathogenic to A. cruentus. Phylogenetic and morphological studies showed that these two clades represent two novel species described here as F. casha and F. curculicola. Both species were shown to have the potential to be pathogenic to A. cruentus during routine greenhouse inoculation tests. While isolations indicate a possible association between these two species and weevils, further research is needed to understand this association and the role of weevils in disease development involving F. casha and F. curculicola in A. cruentus.

Topically delivered 22 nt siRNAs enhance RNAi silencing of endogenous genes in two species

Main conclusion 22 nt siRNAs applied to leaves induce production of transitive sRNAs for targeted genes and can enhance local silencing. Systemic silencing was only observed for a GFP transgene. Abstract RNA interference (RNAi) is a gene silencing mechanism important in regulating gene expression during plant development, response to the environment and defense. Better understanding of the molecular mechanisms of this pathway may lead to future strategies to improve crop traits of value. An abrasion method to deliver siRNAs into leaf cells of intact plants was used to investigate the activities of 21 and 22 nt siRNAs in silencing genes in Nicotiana benthamiana and Amaranthus cruentus. We confirmed that both 21 and 22 nt siRNAs were able to silence a green fluorescent protein (GFP) transgene in treated leaves of N. benthamiana, but systemic silencing of GFP occurred only when the guide strand contained 22 nt. Silencing in the treated leaves of N. benthamiana was demonstrated for three endogenous genes: magnesium cheletase subunit I (CHL-I), magnesium cheletase subunit H (CHL-H), and GENOMES UNCOUPLED4 (GUN4). However, systemic silencing of these endogenous genes was not observed. Very high levels of transitive siRNAs were produced for GFP in response to treatment with 22 nt siRNAs but only low levels were produced in response to a 21 nt siRNA. The endogenous genes tested also produced transitive siRNAs in response to 22 nt siRNAs. 22 nt siRNAs produced greater local silencing phenotypes than 21 nt siRNAs for three of the genes. These special properties of 22 nt siRNAs were also observed for the CHL-H gene in A. cruentus. These experiments suggest a functional role for transitive siRNAs in amplifying the RNAi response.

MPDB 2.0: a large scale and integrated medicinal plant database of Bangladesh

Objective MPDB 2.0 is built to be the continuation of MPDB 1.0, to serve as a more comprehensive data repertoire for Bangladeshi medicinal plants, and to provide a user-friendly interface for researchers, health practitioners, drug developers, and students who wish to study the various medicinal & nutritive plants scattered around Bangladesh and the underlying phytochemicals contributing to their efficacy in Bangladeshi folk medicine. Results MPDB 2.0 database (https://www.medicinalplantbd.com/) comprises a collection of more than five hundred Bangladeshi medicinal plants, alongside a record of their corresponding scientific, family, and local names together with their utilized parts, information regarding ailments, active compounds, and PubMed ID of related publications. While medicinal plants are not limited to the borders of any country, Bangladesh and its Southeast Asian neighbors do boast a huge collection of potent medicinal plants with considerable folk-medicinal history compared to most other countries in the world. Development of MPDB 2.0 has been highly focused upon human diseases, albeit many of the plants indexed here can serve in developing biofuel (e.g.: Jatropha curcas used in biofuel) or bioremediation technologies (e.g.: Amaranthus cruentus helps to reduce cadmium level in soil) or nutritive diets (Terminalia chebula can be used in nutritive diets) or cosmetics (Aloe vera used in cosmetics), etc.