Effect of ultraviolet-B radiation on biomass production, lipid peroxidation, reactive oxygen species, and antioxidants in Withania somnifera

Sickle cell disease promotes hemolytic anemia and occlusion of small blood vessels due to the presence of high concentrations of hemoglobin S, resulting in increased production of reactive oxygen species and decreased antioxidant defense capacity. The aim of this study was to evaluate the protective action of a standardized extract of Ginkgo biloba (EGb 761), selected due to its high content of flavonoids and terpenoids, in erythrocytes of patients with sickle cell anemia (HbSS, SS erythrocytes) subjected to oxidative stress using tert-butylhydroperoxide or 2,2-azobis-(amidinepropane)-dihydrochloride, in vitro. Hemolysis indexes, reduced glutathione, methemoglobin concentrations, lipid peroxidation, and intracellular reactive oxygen species were determined. SS erythrocytes displayed increased rates of oxidation of hemoglobin and membrane lipid peroxidation compared to normal erythrocytes (HbAA, AA erythrocytes), and the concentration of EGb 761 necessary to achieve the same antioxidant effect in SS erythrocytes was at least two times higher than in normal ones, inhibiting the formation of intracellular reactive oxygen species (IC50 of 13.6 µg/mL), partially preventing lipid peroxidation (IC50 of 242.5 µg/mL) and preventing hemolysis (IC50 of 10.5 µg/mL). Thus, EGb 761 has a beneficial effect on the oxidative status of SS erythrocytes. Moreover, EGb 761 failed to prevent oxidation of hemoglobin and reduced glutathione at the concentrations examined.

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Effects of sodium pyruvate on viability, synthesis of reactive oxygen species, lipid peroxidation and DNA integrity of cryopreserved bovine sperm

Animal Reproduction Science ◽

10.1016/j.anireprosci.2017.07.017 ◽

2017 ◽

Vol 185 ◽

pp. 18-27 ◽

Cited By ~ 7

Author(s):

F. Korkmaz ◽

E. Malama ◽

M. Siuda ◽

C. Leiding ◽

H. Bollwein

Keyword(s):

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Reactive Oxygen ◽

Dna Integrity ◽

Oxygen Species ◽

Sodium Pyruvate ◽

Bovine Sperm

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Antioxidant effect of Mn2+ on capacitation and acrosome reaction of fresh and chilled cattle bull semen

Veterinary Science Development ◽

10.4081/vsd.2011.3479 ◽

2012 ◽

Vol 1 (1) ◽

pp. 18

Author(s):

Amrit Kaur Bansal ◽

Ranjna Sundhey Cheema ◽

Vinod Kumar Gandotra

Keyword(s):

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Acrosome Reaction ◽

Reactive Oxygen ◽

Antioxidant Effect ◽

Oxygen Species ◽

Sperm Capacitation ◽

Bull Semen

The aim of this paper was to investigate the antioxidant effect of Mn2+ (200 mM) on the sperm capacitation and acrosome reaction of fresh and chilled cattle bull semen. It has been found that Mn2+ supplementation improves (P≤0.05) the motility at 0, 2, 4 and 6 h of incubation. MDA (malondialdehyde), end product of lipid peroxidation, decreases significantly (P≤0.05) with the supplementation of manganese at 0- and 6-hr of incubation both in fresh and chilled semen. Manganese also increases acrosome reaction significantly (P≤0.05) both in fresh and chilled semen at 0, 4 and 6 h of incubation. Therefore, our findings suggest the role of Mn2+supplementation in improving the quality of cattle bull semen by its scavenging property<em> i.e.</em> reduction in the production of reactive oxygen species during its storage at 4°C or incubation at 37°C for capacitation.

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