Effects of sodium pyruvate on viability, synthesis of reactive oxygen species, lipid peroxidation and DNA integrity of cryopreserved bovine sperm

Reactive oxygen species overgeneration as a side effect of semen cryopreservation may lead to lipid peroxidation, protein degradation, DNA fragmentation and cell death, resulting in a decrease of sperm survival and fertilisation ability. Lycopene has been proposed as a potential supplement to semen extenders because of its antioxidant properties. The aim of this study was to evaluate the effects of lycopene on the structural integrity, functional activity and selected oxidative stress parameters of cryopreserved bovine sperm. Thirty bovine ejaculates were split into two aliquots and diluted with a commercial semen extender supplemented with 1.5 mmol/l lycopene or containing no supplement (control), cooled down to 4 °C, frozen and kept in liquid nitrogen. Prior to experiments, frozen straws were thawed at 37 °C for 20 s. Lycopene addition resulted in a higher sperm motility (P < 0.001), progressive motility (P < 0.001) and all secondary motion characteristics (P < 0.001 with respect to the average path velocity, linear velocity, velocity of curvilinear motion, beat cross frequency, path straightness and linearity; P < 0.01 in the case of the amplitude of lateral head displacement). Furthermore, lycopene exhibited protective effects on the sperm membrane (P < 0.05) and acrosomal (P < 0.01) integrity in comparison to control. An assay for metabolic function revealed that lycopene supplementation to the cryopreservation medium resulted in a higher preservation of the sperm mitochondrial activity (P < 0.001). Reactive oxygen species production as well as intracellular superoxide generation were decreased following lycopene addition (P < 0.01 in the case of reactive oxygen species; P < 0.001 with respect to superoxide production). Finally, the presence of lycopene led to a decrease in protein carbonyl production (P < 0.01), lipid peroxidation (P < 0.001) as well as oxidative DNA damage (P < 0.05) when compared to control. In conclusion, lycopene exhibited significant reactive oxygen species-trapping and antioxidant properties which may prevent oxidative damage to frozen-thawed sperm, and, thus, enhance the post-thaw vitality of male reproductive cells in cattle breeding.

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Lipid peroxidation, mitochondrial membrane potential and DNA integrity of spermatozoa in relation to intracellular reactive oxygen species in liquid and frozen-thawed buffalo semen

Animal Reproduction Science ◽

10.1016/j.anireprosci.2008.10.002 ◽

2009 ◽

Vol 114 (1-3) ◽

pp. 125-134 ◽

Cited By ~ 71

Author(s):

G. Kadirvel ◽

Satish Kumar ◽

A. Kumaresan

Keyword(s):

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Membrane Potential ◽

Mitochondrial Membrane Potential ◽

Mitochondrial Membrane ◽

Reactive Oxygen ◽

Intracellular Reactive Oxygen Species ◽

Dna Integrity ◽

Oxygen Species ◽

Buffalo Semen

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Relationship between reactive oxygen species production and lipid peroxidation in human sperm suspensions and their association with sperm function

Fertility and Sterility ◽

10.1016/j.fertnstert.2004.11.031 ◽

2005 ◽

Vol 83 (4) ◽

pp. 929-936 ◽

Cited By ~ 34

Author(s):

Andrew Colin Williams ◽

William Christopher Liberty Ford

Keyword(s):

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Reactive Oxygen Species Production ◽

Reactive Oxygen ◽

Human Sperm ◽

Sperm Function ◽

Oxygen Species ◽

Species Production

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Ginkgo biloba extract (EGb 761) attenuates oxidative stress induction in erythrocytes of sickle cell disease patients

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502012000400009 ◽

2012 ◽

Vol 48 (4) ◽

pp. 659-665 ◽

Cited By ~ 2

Author(s):

Aline Emmer Ferreira Furman ◽

Railson Henneberg ◽

Priscila Bacarin Hermann ◽

Maria Suely Soares Leonart ◽

Aguinaldo José do Nascimento

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Sickle Cell ◽

Reduced Glutathione ◽

Reactive Oxygen ◽

Intracellular Reactive Oxygen Species ◽

Oxygen Species ◽

Egb 761 ◽

Cell Disease

Sickle cell disease promotes hemolytic anemia and occlusion of small blood vessels due to the presence of high concentrations of hemoglobin S, resulting in increased production of reactive oxygen species and decreased antioxidant defense capacity. The aim of this study was to evaluate the protective action of a standardized extract of Ginkgo biloba (EGb 761), selected due to its high content of flavonoids and terpenoids, in erythrocytes of patients with sickle cell anemia (HbSS, SS erythrocytes) subjected to oxidative stress using tert-butylhydroperoxide or 2,2-azobis-(amidinepropane)-dihydrochloride, in vitro. Hemolysis indexes, reduced glutathione, methemoglobin concentrations, lipid peroxidation, and intracellular reactive oxygen species were determined. SS erythrocytes displayed increased rates of oxidation of hemoglobin and membrane lipid peroxidation compared to normal erythrocytes (HbAA, AA erythrocytes), and the concentration of EGb 761 necessary to achieve the same antioxidant effect in SS erythrocytes was at least two times higher than in normal ones, inhibiting the formation of intracellular reactive oxygen species (IC50 of 13.6 µg/mL), partially preventing lipid peroxidation (IC50 of 242.5 µg/mL) and preventing hemolysis (IC50 of 10.5 µg/mL). Thus, EGb 761 has a beneficial effect on the oxidative status of SS erythrocytes. Moreover, EGb 761 failed to prevent oxidation of hemoglobin and reduced glutathione at the concentrations examined.

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Antioxidant effect of Mn2+ on capacitation and acrosome reaction of fresh and chilled cattle bull semen

Veterinary Science Development ◽

10.4081/vsd.2011.3479 ◽

2012 ◽

Vol 1 (1) ◽

pp. 18

Author(s):

Amrit Kaur Bansal ◽

Ranjna Sundhey Cheema ◽

Vinod Kumar Gandotra

Keyword(s):

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Acrosome Reaction ◽

Reactive Oxygen ◽

Antioxidant Effect ◽

Oxygen Species ◽

Sperm Capacitation ◽

Bull Semen

The aim of this paper was to investigate the antioxidant effect of Mn2+ (200 mM) on the sperm capacitation and acrosome reaction of fresh and chilled cattle bull semen. It has been found that Mn2+ supplementation improves (P≤0.05) the motility at 0, 2, 4 and 6 h of incubation. MDA (malondialdehyde), end product of lipid peroxidation, decreases significantly (P≤0.05) with the supplementation of manganese at 0- and 6-hr of incubation both in fresh and chilled semen. Manganese also increases acrosome reaction significantly (P≤0.05) both in fresh and chilled semen at 0, 4 and 6 h of incubation. Therefore, our findings suggest the role of Mn2+supplementation in improving the quality of cattle bull semen by its scavenging property<em> i.e.</em> reduction in the production of reactive oxygen species during its storage at 4°C or incubation at 37°C for capacitation.

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NADPH Oxidase Activity and Reactive Oxygen Species Production in Brain and Kidney of Adult Male Hypertensive Ren-2 Transgenic Rats

Physiological Research ◽

10.33549/physiolres.933254 ◽

2015 ◽

pp. 849-856 ◽

Cited By ~ 5

Author(s):

M. VOKURKOVÁ ◽

H. RAUCHOVÁ ◽

L. ŘEZÁČOVÁ ◽

I. VANĚČKOVÁ ◽

J. ZICHA

Keyword(s):

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Nadph Oxidase ◽

Medulla Oblongata ◽

Renal Cortex ◽

Reactive Oxygen ◽

Male Rats ◽

Ventrolateral Medulla ◽

Oxygen Species ◽

O2 Production

Hypothalamic paraventricular nucleus (PVN) and rostral ventrolateral medulla (RVLM) play an important role in brain control of blood pressure (BP). One of the important mechanisms involved in the pathogenesis of hypertension is the elevation of reactive oxygen species (ROS) production by nicotine adenine dinucleotide phosphate (NADPH) oxidase. The aim of our present study was to investigate NADPH oxidase-mediated superoxide (O2-) production and to search for the signs of lipid peroxidation in hypothalamus and medulla oblongata as well as in renal medulla and cortex of hypertensive male rats transgenic for the murine Ren-2 renin gene (Ren-2 TGR) and their age-matched normotensive controls ‒ Hannover Sprague Dawley rats (HanSD). We found no difference in the activity of NADPH oxidase measured as a lucigenin-mediated O2- production in the hypothalamus and medulla oblongata. However, we observed significantly elevated NADPH oxidase in both renal cortex and medulla of Ren-2 TGR compared with HanSD. Losartan (LOS) treatment (10 mg/kg body weight/day) for 2 months (Ren-2 TGR+LOS) did not change NADPH oxidase-dependent O2- production in the kidney. We detected significantly elevated indirect markers of lipid peroxidation measured as thiobarbituric acid-reactive substances (TBARS) in Ren-2 TGR, while they were significantly decreased in Ren-2 TGR+LOS. In conclusion, the present study shows increased NADPH oxidase activities in renal cortex and medulla with significantly increased TBARS in renal cortex. No significant changes of NADPH oxidase and markers of lipid peroxidation were detected in the studied brain regions.

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