scholarly journals Development of an in vitro system for functional studies of ovarian follicular cells in European sea bass (Dicentrarchus labrax)

2012 ◽  
Vol 65 (2) ◽  
pp. 273-286 ◽  
Author(s):  
B. Crespo ◽  
S. Zanuy ◽  
A. Gómez
Keyword(s):  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Follicular Cells ◽  
European Sea Bass ◽  
In Vitro System ◽  
Functional Studies

In vitro effect of leptin on somatolactin release in the European sea bass (Dicentrarchus labrax): dependence on the reproductive status and interaction with NPY and GnRH

2003 ◽  
Vol 132 (2) ◽  
pp. 284-292 ◽  
Author(s):  
Pierre Peyon ◽  
Silvia Vega-Rubı́n de Celis ◽  
Pedro Gómez-Requeni ◽  
Silvia Zanuy ◽  
Jaume Pérez-Sánchez ◽  
...  
Keyword(s):  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Reproductive Status ◽  
European Sea Bass ◽  
Vitro Effect

scholarly journals Identification and Regulation of Interleukin-17 (IL-17) Family Ligands in the Teleost Fish European Sea Bass

2020 ◽  
Vol 21 (7) ◽  
pp. 2439 ◽  
Author(s):  
Carmen González-Fernández ◽  
Elena Chaves-Pozo ◽  
Alberto Cuesta
Keyword(s):  
Regulation Of Gene Expression ◽  
Dicentrarchus Labrax ◽  
Head Kidney ◽  
Sea Bass ◽  
Interleukin 17 ◽  
Target Tissue ◽  
Mrna Levels ◽  
European Sea Bass ◽  
The Brain

Interleukin-17 (IL-17) cytokine comprises a family of six ligands in mammals with proinflammatory functions, having an important role in autoimmune disorders and against bacterial, viral, and fungal pathogens. While IL-17A and IL-17F ligands are mainly produced by Th cells (Th17 cells), the rest of the ligands are expressed by other immune and non-immune cells and have different functions. The identification of IL-17 ligands in fish has revealed the presence of six members, counterparts to mammalian ones, and a teleost-specific form, the fish IL-17N. However, tissue distribution, the regulation of gene expression, and scarce bioactivity assays point to similar functions compared to mammalian ones, though this yet to be investigated and confirmed. Thus, we have identified seven IL-17 ligands in the teleost European sea bass (Dicentrarchus labrax), for the first time, corresponding to IL-17A/F1, IL-17A/F2, IL-17A/F3, IL-17C1, IL-17C2, IL-17D, and IL-17N, according to the predicted protein sequences and phylogenetic analysis. They are constitutively and widely transcribed in sea bass tissues, with some of them being mainly expressed in the thymus, brain or intestine. Upon in vitro stimulation of head-kidney leucocytes, the mRNA levels of all sea bass IL-17 ligands were up-regulated by phytohemagglutinin treatment, a well-known T cell mitogen, suggesting a major expression in T lymphocytes. By contrast, the infection of sea bass juveniles with nodavirus (NNV), a very pathogenic virus for this fish species, resulted in the up-regulation of the transcription of IL-17C1 in the head-kidney and of IL-17C1 and IL-17D in the brain, the target tissue for NNV replication. By contrast, NNV infection led to a down-regulated transcription of IL-17A/F1, IL-17A/F2, IL-17C1, IL-17C2, and IL-17D in the head-kidney and of IL-17A/F1 and IL-17A/F3 in the brain. The data are discussed accordingly with the IL-17 ligand expression and the immune response under the different situations tested.

scholarly journals In vitro evaluation of cytotoxic and genotoxic effects of Di(2-ethylhexyl)-phthalate (DEHP) on European sea bass (Dicentrarchus labrax) embryonic cell line

2019 ◽  
Vol 56 ◽  
pp. 118-125 ◽  
Author(s):  
Chiara Molino ◽  
Silvia Filippi ◽  
Gerardo Antonio Stoppiello ◽  
Roberta Meschini ◽  
Dario Angeletti
Keyword(s):  
Cell Line ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Embryonic Cell ◽  
Genotoxic Effects ◽  
European Sea Bass ◽  
In Vitro Evaluation ◽  
Embryonic Cell Line ◽  
Ethylhexyl Phthalate

scholarly journals Individual variation in whole-animal hypoxia tolerance is associated with cardiac hypoxia tolerance in a marine teleost

2016 ◽  
Vol 12 (1) ◽  
pp. 20150708 ◽  
Author(s):  
William Joyce ◽  
Karlina Ozolina ◽  
Florian Mauduit ◽  
Hélène Ollivier ◽  
Guy Claireaux ◽  
...  
Keyword(s):  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Cardiac Performance ◽  
Hypoxia Tolerance ◽  
Hypoxic Exposure ◽  
European Sea Bass ◽  
Contraction And Relaxation ◽  
Cardiac Hypoxia ◽  
Fish Survival

Hypoxia is a pervasive problem in coastal environments and is predicted to have enduring impacts on aquatic ecosystems. Intraspecific variation in hypoxia tolerance is well documented in fish; however, the factors underlying this variation remain unknown. Here, we investigate the role of the heart in individual hypoxia tolerance of the European sea bass ( Dicentrarchus labrax ). We found individual whole-animal hypoxia tolerance is a stable trait in sea bass for more than 18 months (duration of study). We next examined in vitro cardiac performance and found myocardial muscle from hypoxia-tolerant individuals generated greater force, with higher rates of contraction and relaxation, than hypoxic-sensitive individuals during hypoxic exposure. Thus, whole-animal hypoxia tolerance is associated with cardiac hypoxia tolerance. As the occurrence of aquatic hypoxia is expected to increase in marine ecosystems, our experimental data suggest that cardiac performance may influence fish survival and distribution.

In vitro immunotoxicological effects of heavy metals on European sea bass ( Dicentrarchus labrax L.) head-kidney leucocytes

2015 ◽  
Vol 47 (1) ◽  
pp. 245-254 ◽  
Author(s):  
Patricia Morcillo ◽  
Héctor Cordero ◽  
José Meseguer ◽  
M. Ángeles Esteban ◽  
Alberto Cuesta
Keyword(s):  
Heavy Metals ◽  
Dicentrarchus Labrax ◽  
Head Kidney ◽  
Sea Bass ◽  
European Sea Bass
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