scholarly journals Identification and Regulation of Interleukin-17 (IL-17) Family Ligands in the Teleost Fish European Sea Bass

2020 ◽  
Vol 21 (7) ◽  
pp. 2439 ◽  
Author(s):  
Carmen González-Fernández ◽  
Elena Chaves-Pozo ◽  
Alberto Cuesta
Keyword(s):  
Regulation Of Gene Expression ◽  
Dicentrarchus Labrax ◽  
Head Kidney ◽  
Sea Bass ◽  
Interleukin 17 ◽  
Target Tissue ◽  
Mrna Levels ◽  
European Sea Bass ◽  
The Brain

Interleukin-17 (IL-17) cytokine comprises a family of six ligands in mammals with proinflammatory functions, having an important role in autoimmune disorders and against bacterial, viral, and fungal pathogens. While IL-17A and IL-17F ligands are mainly produced by Th cells (Th17 cells), the rest of the ligands are expressed by other immune and non-immune cells and have different functions. The identification of IL-17 ligands in fish has revealed the presence of six members, counterparts to mammalian ones, and a teleost-specific form, the fish IL-17N. However, tissue distribution, the regulation of gene expression, and scarce bioactivity assays point to similar functions compared to mammalian ones, though this yet to be investigated and confirmed. Thus, we have identified seven IL-17 ligands in the teleost European sea bass (Dicentrarchus labrax), for the first time, corresponding to IL-17A/F1, IL-17A/F2, IL-17A/F3, IL-17C1, IL-17C2, IL-17D, and IL-17N, according to the predicted protein sequences and phylogenetic analysis. They are constitutively and widely transcribed in sea bass tissues, with some of them being mainly expressed in the thymus, brain or intestine. Upon in vitro stimulation of head-kidney leucocytes, the mRNA levels of all sea bass IL-17 ligands were up-regulated by phytohemagglutinin treatment, a well-known T cell mitogen, suggesting a major expression in T lymphocytes. By contrast, the infection of sea bass juveniles with nodavirus (NNV), a very pathogenic virus for this fish species, resulted in the up-regulation of the transcription of IL-17C1 in the head-kidney and of IL-17C1 and IL-17D in the brain, the target tissue for NNV replication. By contrast, NNV infection led to a down-regulated transcription of IL-17A/F1, IL-17A/F2, IL-17C1, IL-17C2, and IL-17D in the head-kidney and of IL-17A/F1 and IL-17A/F3 in the brain. The data are discussed accordingly with the IL-17 ligand expression and the immune response under the different situations tested.

In vitro immunotoxicological effects of heavy metals on European sea bass ( Dicentrarchus labrax L.) head-kidney leucocytes

2015 ◽  
Vol 47 (1) ◽  
pp. 245-254 ◽  
Author(s):  
Patricia Morcillo ◽  
Héctor Cordero ◽  
José Meseguer ◽  
M. Ángeles Esteban ◽  
Alberto Cuesta
Keyword(s):  
Heavy Metals ◽  
Dicentrarchus Labrax ◽  
Head Kidney ◽  
Sea Bass ◽  
European Sea Bass

scholarly journals Potential Involvement of lncRNAs in the Modulation of the Transcriptome Response to Nodavirus Challenge in European Sea Bass (Dicentrarchus labrax L.)

Biology ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 165
Author(s):  
Patricia Pereiro ◽  
Raquel Lama ◽  
Rebeca Moreira ◽  
Valentina Valenzuela-Muñoz ◽  
Cristian Gallardo-Escárate ◽  
...  
Keyword(s):  
Immune Response ◽  
Dicentrarchus Labrax ◽  
Head Kidney ◽  
Enrichment Analysis ◽  
Sea Bass ◽  
Integrated Analysis ◽  
European Sea Bass ◽  
Commercial Species ◽  
Number Of Publications ◽  
Systematic Identification

Long noncoding RNAs (lncRNAs) are being increasingly recognised as key modulators of various biological mechanisms, including the immune response. Although investigations in teleosts are still lagging behind those conducted in mammals, current research indicates that lncRNAs play a pivotal role in the response of fish to a variety of pathogens. During the last several years, interest in lncRNAs has increased considerably, and a small but notable number of publications have reported the modulation of the lncRNA profile in some fish species after pathogen challenge. This study was the first to identify lncRNAs in the commercial species European sea bass. A total of 12,158 potential lncRNAs were detected in the head kidney and brain. We found that some lncRNAs were not common for both tissues, and these lncRNAs were located near coding genes that are primarily involved in tissue-specific processes, reflecting a degree of cellular specialisation in the synthesis of lncRNAs. Moreover, lncRNA modulation was analysed in both tissues at 24 and 72 h after infection with nodavirus. Enrichment analysis of the neighbouring coding genes of the modulated lncRNAs revealed many terms related to the immune response and viral infectivity but also related to the stress response. An integrated analysis of the lncRNAs and coding genes showed a strong correlation between the expression of the lncRNAs and their flanking coding genes. Our study represents the first systematic identification of lncRNAs in European sea bass and provides evidence regarding the involvement of these lncRNAs in the response to nodavirus.

scholarly journals Intestinal B0AT1 (SLC6A19) and PEPT1 (SLC15A1) mRNA levels in European sea bass (Dicentrarchus labrax) reared in fresh water and fed fish and plant protein sources

2015 ◽  
Vol 4 ◽  
Author(s):  
Simona Rimoldi ◽  
Elena Bossi ◽  
Sheenan Harpaz ◽  
Anna Giulia Cattaneo ◽  
Giovanni Bernardini ◽  
...  
Keyword(s):  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Fish Growth ◽  
Mrna Levels ◽  
Feed Conversion ◽  
European Sea Bass ◽  
Protein Sources ◽  
Plant Protein Sources

AbstractThe objective of the present study was to examine the effect of diets with descending fish meal (FM) inclusion levels and the addition of salt to the diet containing the lowest FM level on growth performances, feed conversion ratio, and intestinal solute carrier family 6 member 19 (SLC6A19) and oligopeptide transporter 1 (PEPT1) transcript levels, in freshwater-adapted European sea bass (Dicentrarchus labrax). We first isolated by molecular cloning and sequenced a full-length cDNA representing the neutral amino acid transporter SLC6A19 in sea bass. The cDNA sequence was deposited in GenBank database (accession no. KC812315). The twelve transmembrane domains and the ‘de novo’ prediction of the three-dimensional structure of SLC6A19 protein (634 amino acids) are presented. We then analysed diet-induced changes in the mRNA copies of SLC6A19 and PEPT1 genes in different portions of sea bass intestine using real-time RT-PCR. Sea bass were fed for 6 weeks on different diets, with ascending levels of fat or descending levels of FM, which was replaced with vegetable meal. The salt-enriched diet was prepared by adding 3 % NaCl to the diet containing 10 % FM. SLC6A19 mRNA in the anterior and posterior intestine of sea bass were not modulated by dietary protein sources and salt supplementation. Conversely, including salt in a diet containing a low FM percentage up-regulated the mRNA copies of PEPT1 in the hindgut. Fish growth correlated positively with the content of FM in the diets. Interestingly, the addition of salt to the diet containing 10 % FM improved feed intake, as well as specific growth rate and feed conversion ratio.

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