Cryoprotection synergism between glycerol and dimethyl sulfoxide improves the mitochondrial transmembrane potential, plasmalemma, acrosomal and DNA integrities, and in vivo fertility of water buffalo (Bubalus bubalis) spermatozoa

Background. Armillaridin (AM) is isolated fromArmillaria mellea. We examined the anticancer activity and radiosensitizing effect on human esophageal cancer cells.Methods. Human squamous cell carcinoma (CE81T/VGH and TE-2) and adenocarcinoma (BE-3 and SKGT-4) cell lines were cultured. The MTT assay was used for cell viability. The cell cycle was analyzed using propidium iodide staining. Mitochondrial transmembrane potential was measured by DiOC6(3) staining. The colony formation assay was performed for estimation of the radiation surviving fraction. Human CE81T/VGH xenografts were established for evaluation of therapeutic activityin vivo.Results. AM inhibited the viability of four human esophageal cancer cell lines with an estimated concentration of 50% inhibition (IC50) which was 3.4–6.9 μM. AM induced a hypoploid cell population and morphological alterations typical of apoptosis in cells. This apoptosis induction was accompanied by a reduction of mitochondrial transmembrane potential. AM accumulated cell cycle at G2/M phase and enhanced the radiosensitivity in CE81T/VGH cells.In vivo, AM inhibited the growth of CE81T/VGH xenografts without significant impact on body weight and white blood cell counts.Conclusion. Armillaridin could inhibit growth and enhance radiosensitivity of human esophageal cancer cells. There might be potential to integrate AM with radiotherapy for esophageal cancer treatment.

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A laparoscopic technique for in vivo observation of ovaries in the water buffalo (Bubalus bubalis)

Veterinary Record ◽

10.1136/vr.111.2.32 ◽

1982 ◽

Vol 111 (2) ◽

pp. 32-34 ◽

Cited By ~ 5

Author(s):

M. Jainudeen ◽

T. Bongso ◽

F. Ahmad ◽

W. Sharifuddin

Keyword(s):

Water Buffalo ◽

Bubalus Bubalis ◽

Laparoscopic Technique

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Programmable fast-freezing method improves the post-thaw motion dynamics, integrities of plasmalemma, mitochondrial transmembrane, DNA and, acrosome, and in vivo fertility of water buffalo (Bubalus bubalis ) spermatozoa

Andrologia ◽

10.1111/and.12733 ◽

2016 ◽

Vol 49 (8) ◽

pp. e12733

Author(s):

H. Khalil Ur Rehman ◽

S. M. H. Andrabi ◽

H. Ahmed ◽

S. A. H. Shah

Keyword(s):

Water Buffalo ◽

Bubalus Bubalis ◽

Freezing Method ◽

Fast Freezing ◽

Motion Dynamics

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Protective Effects of Hyperoside from Hawthorn against Endothelia Cell Apoptosis Induced by H2O2

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.343-344.1007 ◽

2011 ◽

Vol 343-344 ◽

pp. 1007-1014

Author(s):

Ya Kun Ge ◽

Xiao Xiang Zheng

Keyword(s):

Endothelial Cell ◽

Transmembrane Potential ◽

Flavonol Glycoside ◽

Mitochondrial Transmembrane Potential ◽

Protective Effects ◽

Cell Protection ◽

Intracellular Ros ◽

Apoptosis And Necrosis ◽

Oxidative Effect

Hyperoside is a flavonol glycoside extraction from Hawthorn (Crataegus), and it has been reported that hyperoside process anti-inflammatory and anti-oxidative effectin vivo. In this paper, we studied the effects of hyperoside on apoptosis, necrosis, intracellular ROS level, mitochondrial transmembrane potential, and expression of Bax and Bcl-2 of endothelial cells subjected to H2O2,. The results showed that hyperoside treatment (50 μmol/L and 200 μmol/L ) significantly reduced apoptosis and necrosis, intracellular ROS and the expression of Bax in endothelial cell subjected to H2O2, and increased mitochondrial transmembrane potential and Bcl-2 expression. In conclusion, the present study indicates that hyperoside is in favor of endothelial cell protection.

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