Patient Self-Administered Abdominal Pressure to Reduce Loop Formation During Minimally Sedated Colonoscopy

2009 ◽  
Vol 55 (5) ◽  
pp. 1429-1433 ◽  
Author(s):  
Yu-Hsi Hsieh ◽  
Kuo-Chih Tseng ◽  
An-Liang Chou
Keyword(s):  
Abdominal Pressure ◽  
Loop Formation

Point Defect Absorption and Dislocation Loop Formation at Grain Boundaries in Hvem-Irradiated Zr and Its Alloys:1. Influence of Solute Addition

1985 ◽  
Vol 43 ◽  
pp. 350-351
Author(s):  
R.A. Herring ◽  
M. Griffiths ◽  
M.H Loretto ◽  
R.E. Smallman
Keyword(s):  
Grain Boundary ◽  
Grain Boundaries ◽  
Point Defect ◽  
Reactor Core ◽  
Solute Concentration ◽  
Nuclear Industry ◽  
Dislocation Loops ◽  
Loop Formation ◽  
Component Material ◽  
Impurity Interaction

Because Zr is used in the nuclear industry to sheath fuel and as structural component material within the reactor core, it is important to understand Zr's point defect properties. In the present work point defect-impurity interaction has been assessed by measuring the influence of grain boundaries on the width of the zone denuded of dislocation loops in a series of irradiated Zr alloys. Electropolished Zr and its alloys have been irradiated using an AEI EM7 HVEM at 1 MeV, ∼675 K and ∼10-6 torr vacuum pressure. During some HVEM irradiations it has been seen that there is a difference in the loop nucleation and growth behaviour adjacent to the grain boundary as compared with the mid-grain region. The width of the region influenced by the presence of the grain boundary should be a function of the irradiation temperature, dose rate, solute concentration and crystallographic orientation.

Influence of intra-abdominal pressure on the flow of chronic colostasis for children

2016 ◽  
Vol 52-53 (3-4) ◽  
pp. 62-67
Author(s):  
V.S. Konoplytsky ◽  
◽  
O.O. Lukiyanets ◽  
Keyword(s):  
Abdominal Pressure

COMPARATIVE EVALUATION OF GUT DECOMPRESSION METHODS IN OPERATIONS ON TOXIC WIDESPREAD PERITONITIS

2019 ◽  
pp. 34-37
Author(s):  
V.I. Midlenko ◽  
N.I. Belonogov ◽  
O.V. Midlenko ◽  
A.L. Charyshkin
Keyword(s):  
Small Intestine ◽  
Treatment Outcomes ◽  
Comparative Evaluation ◽  
Comparative Assessment ◽  
Abdominal Pressure ◽  
Treatment Results ◽  
Intestinal Decompression ◽  
Bowel Decompression

The aim of the paper is to conduct a comparative assessment of bowel decompression methods in operations on toxic widespread peritonitis. Materials and Methods. The authors examined 162 patients with widespread peritonitis of various etiologies. Using generally accepted methods, they compared treatment results using one of the three methods of intestinal decompression: cecostomy, nasointestinal intubation, and cecostomy with intubation of the small intestine. Results. It was detected that in case of small intestine intubation through the cecostomy (in comparison with other methods), intra-abdominal pressure returned to normal on the 2nd day, and in case of cecostomy or nasointestinal intubation – on the 5th and 7th day, respectively. Also, decrease in intoxication indicators was observed 2 days earlier than usual. Conclusion. Intubation of the small intestine through the cecostoma provides the most adequate intestinal decompression in patients with advanced toxic peritonitis. Early normalization of intra-abdominal pressure in patients with advanced toxic peritonitis improves treatment outcomes. Keywords: widespread peritonitis, intestinal decompression. Цель – сравнительная оценка способов декомпрессии кишечника при операциях по поводу распространённого перитонита в токсической стадии. Материалы и методы. Наблюдали 162 пациента с распространённым перитонитом различной этиологии. С использованием общепринятых методик сравнивались результаты лечения при применении одного из трёх способов декомпрессии кишечника: цекостомии, назоинтестинальной интубации и наложения цекостомы с интубацией тонкой кишки. Результаты. Установлено, что при использовании интубации тонкого кишечника через цекостому, в сравнении с другими применявшимися нами способами, внутрибрюшное давление нормализовалось на второй день, а при использовании цекостомии или назоинтестинальной интубации – на 5-й и 7-й дни соответственно. Также на 2 сут раньше происходило снижение показателей интоксикации. Выводы. Наиболее адекватную декомпрессию кишечника у пациентов с распространённым перитонитом в токсической стадии обеспечивает интубация тонкого кишечника через цекостому. Ранняя нормализация внутрибрюшного давления у пациентов с распространённым перитонитом в токсической стадии позволяет улучшить результаты лечения. Ключевые слова: распространённый перитонит, декомпрессия кишечника.

scholarly journals Inhibition of CRISPR-Cas12a DNA targeting by nucleosomes and chromatin

2021 ◽  
Vol 7 (11) ◽  
pp. eabd6030
Author(s):  
Isabel Strohkendl ◽  
Fatema A. Saifuddin ◽  
Bryan A. Gibson ◽  
Michael K. Rosen ◽  
Rick Russell ◽  
...  
Keyword(s):  
Histone Modifications ◽  
Dna Cleavage ◽  
Genome Engineering ◽  
Eukaryotic Cells ◽  
Loop Formation ◽  
Chromatin Compaction ◽  
Dna Targeting ◽  
Genomic Regions ◽  
Dna Bendability ◽  
Nucleosome Arrays

Genome engineering nucleases must access chromatinized DNA. Here, we investigate how AsCas12a cleaves DNA within human nucleosomes and phase-condensed nucleosome arrays. Using quantitative kinetics approaches, we show that dynamic nucleosome unwrapping regulates target accessibility to Cas12a and determines the extent to which both steps of binding—PAM recognition and R-loop formation—are inhibited by the nucleosome. Relaxing DNA wrapping within the nucleosome by reducing DNA bendability, adding histone modifications, or introducing target-proximal dCas9 enhances DNA cleavage rates over 10-fold. Unexpectedly, Cas12a readily cleaves internucleosomal linker DNA within chromatin-like, phase-separated nucleosome arrays. DNA targeting is reduced only ~5-fold due to neighboring nucleosomes and chromatin compaction. This work explains the observation that on-target cleavage within nucleosomes occurs less often than off-target cleavage within nucleosome-depleted genomic regions in cells. We conclude that nucleosome unwrapping regulates accessibility to CRISPR-Cas nucleases and propose that increasing nucleosome breathing dynamics will improve DNA targeting in eukaryotic cells.

Role of Intra-Abdominal Pressure as a Prognostic Marker and Its Correlation with APACHE II and SOFA Scores in Patients of Acute Pancreatitis

2021 ◽  
Author(s):  
Arun Kumar Gupta ◽  
Ekta Yadav ◽  
Nikhil Gupta ◽  
Raghav Yelamanchi ◽  
Lalit Kumar Bansal ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Prognostic Marker ◽  
Apache Ii ◽  
Abdominal Pressure

scholarly journals SP723INTRA ABDOMINAL PRESSURE AND RENAL ARTERY BLOOD FLOW IN THE EARLY POST TRANSPLANTATION PERIOD

2018 ◽  
Vol 33 (suppl_1) ◽  
pp. i589-i590
Author(s):  
Armando Coca ◽  
Ana Lucia Valencia ◽  
Cristina Ferrer ◽  
Pablo Gonzalez ◽  
Miriam Martinez ◽  
...  
Keyword(s):  
Blood Flow ◽  
Renal Artery ◽  
Abdominal Pressure ◽  
Artery Blood Flow ◽  
Post Transplantation

scholarly journals Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Bo Zhang ◽  
Diyin Luo ◽  
Yu Li ◽  
Vanja Perčulija ◽  
Jing Chen ◽  
...  
Keyword(s):  
Genome Editing ◽  
Dna Cleavage ◽  
Binary Complex ◽  
Loop Formation ◽  
Dna Duplex ◽  
Target Dna ◽  
Before And After ◽  
Dna Strand ◽  
Type V ◽  
Functionally Diverse

AbstractCas12i is a newly identified member of the functionally diverse type V CRISPR-Cas effectors. Although Cas12i has the potential to serve as genome-editing tool, its structural and functional characteristics need to be investigated in more detail before effective application. Here we report the crystal structures of the Cas12i1 R-loop complexes before and after target DNA cleavage to elucidate the mechanisms underlying target DNA duplex unwinding, R-loop formation and cis cleavage. The structure of the R-loop complex after target DNA cleavage also provides information regarding trans cleavage. Besides, we report a crystal structure of the Cas12i1 binary complex interacting with a pseudo target oligonucleotide, which mimics target interrogation. Upon target DNA duplex binding, the Cas12i1 PAM-interacting cleft undergoes a remarkable open-to-closed adjustment. Notably, a zipper motif in the Helical-I domain facilitates unzipping of the target DNA duplex. Formation of the 19-bp crRNA-target DNA strand heteroduplex in the R-loop complexes triggers a conformational rearrangement and unleashes the DNase activity. This study provides valuable insights for developing Cas12i1 into a reliable genome-editing tool.

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