Deposits of biofluid droplets on surfaces (such as respiratory droplets formed during an expiratory event fallen on surfaces) are composed of the water based salt protein solution that may also contain an infection (bacterial/viral). The final patterns of the deposit formed are dictated by the composition of the fluid and flow dynamics within the droplet. This work reports the spatio temporal, topological regulation of deposits of respiratory fluid droplets and control of motility of bacteria by tweaking flow inside droplets using non contact vapor mediated interactions. When evaporated on a glass surface, respiratory droplets form haphazard multiscale dendritic, cruciform shaped precipitates using vapor mediation as a tool to control these deposits at the level of nano, micro, millimeter scales. We morphologically control dendrite orientation, size and subsequently suppress cruciform-shaped crystals. The nucleation sites are controlled via preferential transfer of solutes in the droplets; thus, achieving control over crystal occurrence and growth dynamics. The active living matter like bacteria is also preferentially segregated with controlled motility without attenuation of its viability and pathogenesis. For the first time, we have experimentally presented a proof of concept to control the motion of live active matter like bacteria in a near nonintrusive manner. The methodology can have ramifications in biomedical applications like disease detection, controlling bacterial motility, and bacterial segregation.
Shifts in the spatio-temporal growth dynamics of shortleaf pine