Analysis of spatio-temporal fungal growth dynamics under different environmental conditions

Abstract Exploring extractable phytochemicals from locally adapted sisal plant vegetation vary seasonally at different locations. This study elaborated proximate composition and phytochemical heterogeneity in sisal due to varying environmental conditions analyzed from five districts, i.e., Chakwal, Khushab, Rawalpindi, Faisalabad, and Layyah in Punjab, Pakistan. Extensive surveying and plant sampling across two years 2017-18 and 2018-19, during mid-spring, summer, autumn, and winter seasons were carried out for understanding the seasonal impact on sisal. The present study was designed in a randomized complete block design (RCBD) and analyzed considering seasonal, yearly, and locational impact. The spatial differences in phytochemicals concentration were strongly associated with environmental conditions prevailing in different seasons. Autumn season reflected saponins, tannins, and flavonoids in higher concentrations during 2018-19 while steroids and terpenoids were higher during spring 2018-19. While Spatio-temporal variations in the proximate analysis were more apparent in different samples collected from different districts. Data recorded for the Khushab district and autumn season reflected the higher composition of a proximate analysis and phytochemical contents as compared to other seasons. Overall, the spatial differences in phytochemicals concentration were strongly associated with soils and environmental conditions prevailing in different seasons in selected districts.

Download Full-text

Modeling Environmental Impacts on Viticultural Ecosystems

International Journal of Agricultural and Environmental Information Systems ◽

10.4018/ijaeis.2017070101 ◽

2017 ◽

Vol 8 (3) ◽

pp. 1-20

Author(s):

Cyril Tissot ◽

Etienne Neethling ◽

Mathias Rouan ◽

Gérard Barbeau ◽

Hervé Quénol ◽

...

Keyword(s):

Environmental Impacts ◽

Environmental Conditions ◽

Management Strategies ◽

Temporal Data ◽

Agent Based ◽

Knowledge Database ◽

Behavioral Dynamics ◽

Regulatory Data ◽

Temporal Data Models ◽

Spatio Temporal

This paper focuses on simulating environmental impacts on grapevine behavioral dynamics and vineyard management strategies. The methodology presented uses technology from geomatics object oriented databases and spatio-temporal data models. Our approach has two principle objectives, first, to simulate grapevine phenology and grape ripening under spatial and temporal environmental conditions and constraints and secondly, to simulate viticultural practices and adaptation strategies under various constraints (environmental, economical, socio-technical). The approach is based on a responsive agent-based structure where environmental conditions and constraints are considered as a set of forcing data (biophysical, socio-economic and regulatory data) that influences the modelled activities. The experiment was conducted in the regulated wine producing appellation Grand Cru “Quarts de Chaume”, situated in the middle Loire Valley, France. All of the methodology, from the implementation of the knowledge database to the analysis of the first simulation, is presented in this paper.

Download Full-text

Quantification of Geotrichum candidum growth in co-culture with lactic acid bacteria

Czech Journal of Food Sciences ◽

10.17221/205/2009-cjfs ◽

2010 ◽

Vol 27 (Special Issue 2) ◽

pp. 18-27 ◽

Cited By ~ 1

Author(s):

A. Hudecová ◽

Ľ. Valík ◽

D. Liptáková

Keyword(s):

Growth Rate ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Filamentous Fungus ◽

Solid Medium ◽

Fungal Growth ◽

Growth Dynamics ◽

Geotrichum Candidum ◽

Inhibitory Effect ◽

Food Mycology

The growth dynamics of filamentous fungus G. candidum was studied during the co-cultivation with the commercial lactic acid bacteria (LAB) culture Fresco. The experiments were carried out in milk and on the surface of a milk agar at the temperature ranging from 5 to 37°C. Ratkowsky model was used to describe the relationships of the fungal growth rate to the temperature during both, single and co-cultivation with LAB in milk. Simultaneous growth of LAB affected significantly the growth rate of the filamentous fungus. The growth of G. candidum was in average 39% slower in the co-culture than in the single cultivation. LAB pre-inoculated and growing in the solid medium did not show any significant inhibitory effect on the surface growth of G. candidum at all tested temperature. The precise data describing the growth of this cheese yeast-like fungus, G. candidum, may fill a gap in the field of quantitative food mycology and may be used for predicting its behavior in real conditions.

Download Full-text

Fungal Biodeterioration of Paper: How are Paper and Book Conservators Dealing with it? An International Survey

Restaurator International Journal for the Preservation of Library and Archival Material ◽

10.1515/rest-2014-0005 ◽

2014 ◽

Vol 35 (2) ◽

Cited By ~ 4

Author(s):

Sílvia O. Sequeira ◽

Eurico J. Cabrita ◽

Maria F. Macedo

Keyword(s):

Environmental Conditions ◽

Preventive Measures ◽

Fungal Growth ◽

Research Topic ◽

International Survey ◽

Relevant Research ◽

Fungal Development

AbstractPaper biodeterioration by fungi has always been a concern in archives, libraries and museums. Several guidelines have been published regarding the prevention of fungal development in paper collections and recovery of affected objects, but what is actually being implemented from the literature by worldwide paper and book conservators? How common is this type of biodeterioration? What needs to be further studied? In order to access this information we conducted an online international questionnaire with participants from 20 different countries. The results show that fungal biodeterioration is highly common in paper collections. All of the respondents already had to deal with paper deteriorated by fungi, and although the vast majority uses active measures to prevent fungal development, most of them have already experienced active fungal infestations. The mainly used preventive measures are the ones concerned with the control of the environmental conditions in storage and display rooms. Drying the affected paper objects and applying 70% ethanol are the most preferred options to stop active fungal growth. The study of non-toxic and safer antifungals is considered here as the most relevant research topic in the area of paper biodeterioration by fungi, meaning that the options currently available are not totally satisfactory.

Download Full-text

Somatic growth dynamics of West Atlantic hawksbill sea turtles: a spatio‐temporal perspective

Ecosphere ◽

10.1002/ecs2.1279 ◽

2016 ◽

Vol 7 (5) ◽

Cited By ~ 16

Author(s):

Karen A. Bjorndal ◽

Milani Chaloupka ◽

Vincent S. Saba ◽

Carlos E. Diez ◽

Robert P. van Dam ◽

...

Keyword(s):

Sea Turtles ◽

Somatic Growth ◽

Growth Dynamics ◽

Temporal Perspective ◽

Spatio Temporal

Download Full-text

Spatio-temporal analysis of locomotion in BALB/cByJ and C57BL/6J mice in different environmental conditions

Behavioural Brain Research ◽

10.1016/j.bbr.2005.10.001 ◽

2006 ◽

Vol 167 (2) ◽

pp. 365-372 ◽

Cited By ~ 18

Author(s):

E LEPICARD ◽

P VENAULT ◽

A ABOURACHID ◽

E PELLE ◽

G CHAPOUTHIER ◽

...

Keyword(s):

Environmental Conditions ◽

Temporal Analysis ◽

Spatio Temporal

Download Full-text

Shifts in the spatio-temporal growth dynamics of shortleaf pine

Environmental and Ecological Statistics ◽

10.1007/s10651-007-0016-1 ◽

2007 ◽

Vol 14 (3) ◽

pp. 207-227 ◽

Cited By ~ 15

Author(s):

Mevin B. Hooten ◽

Christopher K. Wikle

Keyword(s):

Growth Dynamics ◽

Shortleaf Pine ◽

Spatio Temporal

Download Full-text

Rhizopus oligosporus and yeast co-cultivation during barley tempeh fermentation—Nutritional impact and real-time PCR quantification of fungal growth dynamics

Food Microbiology ◽

10.1016/j.fm.2006.06.007 ◽

2007 ◽

Vol 24 (4) ◽

pp. 393-402 ◽

Cited By ~ 19

Author(s):

Xin Mei Feng ◽

Volkmar Passoth ◽

Charlotte Eklund-Jonsson ◽

Marie Larsson Alminger ◽

Johan Schnürer

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Fungal Growth ◽

Growth Dynamics ◽

Rhizopus Oligosporus

Download Full-text

Comprehensive analysis of Verticillium nonalfalfae in silico secretome uncovers putative effector proteins expressed during hop invasion

10.1101/237255 ◽

2017 ◽

Author(s):

Kristina Marton ◽

Marko Flajšman ◽

Sebastjan Radišek ◽

Katarina Košmelj ◽

Jernej Jakše ◽

...

Keyword(s):

Verticillium Wilt ◽

Sequence Similarity ◽

Vascular Plant ◽

Xylem Sap ◽

Fungal Growth ◽

Effector Proteins ◽

Specific Gene ◽

In Planta ◽

Verticillium Nonalfalfae ◽

Spatio Temporal

AbstractBackgroundThe vascular plant pathogen Verticillium nonalfalfae causes Verticillium wilt in several important crops. VnaSSP4.2 was recently discovered as a V. nonalfalfae virulence effector protein in the xylem sap of infected hop. Here, we expanded our search for candidate secreted effector proteins (CSEPs) in the V. nonalfalfae predicted secretome using a bioinformatic pipeline built on V. nonalfalfae genome data, RNA-Seq and proteomic studies of the interaction with hop.ResultsThe secretome, rich in carbohydrate active enzymes, proteases, redox proteins and proteins involved in secondary metabolism, cellular processing and signaling, includes 263 CSEPs. Several homologs of known fungal effectors (LysM, NLPs, Hce2, Cerato-platanins, Cyanovirin-N lectins, hydrophobins and CFEM domain containing proteins) and avirulence determinants in the PHI database (Avr-Pita1 and MgSM1) were found. The majority of CSEPs were non-annotated and were narrowed down to 44 top priority candidates based on their likelihood of being effectors. These were examined by spatio-temporal gene expression profiling of infected hop. Among the highest in planta expressed CSEPs, five deletion mutants were tested in pathogenicity assays. A deletion mutant of VnaUn.279, a lethal pathotype specific gene with sequence similarity to SAM-dependent methyltransferase (LaeA), had lower infectivity and showed highly reduced virulence, but no changes in morphology, fungal growth or conidiation were observed.ConclusionsSeveral putative secreted effector proteins that probably contribute to V. nonalfalfae colonization of hop were identified in this study. Among them, LaeA gene homolog was found to act as a potential novel virulence effector of V. nonalfalfae. The combined results will serve for future characterization of V. nonalfalfae effectors, which will advance our understanding of Verticillium wilt disease.

Download Full-text

Metabolism of phosphorus compoundsand taxonomic position of the Aspergillus niger AM1 mold

Butlerov Communications ◽

10.37952/roi-jbc-01/20-62-6-98 ◽

2020 ◽

Vol 62 (6) ◽

pp. 98-124

Author(s):

Anton Z. Mindubaev ◽

◽

Elena K. Badeeva ◽

Salima T. Minzanova ◽

Lubov’ G. Mironova ◽

...

Keyword(s):

Aspergillus Niger ◽

Environmental Pollutants ◽

Fungal Growth ◽

Growth Dynamics ◽

Sole Source ◽

White Phosphorus ◽

Phosphorus Compounds ◽

Phosphoric Acid Ester ◽

Relationship Of ◽

First Time

White phosphorus is one of the most dangerous environmental pollutants. However, it is used in industry and for military purposes; therefore, it is impossible to overlook the fact that this substance is constantly released into the environment. In our works, cultures of microorganisms growing in media with a content of white phosphorus up to 1% were obtained for the first time. This exceeds the TLV in wastewater by 5000 times! These cultures are unique, and they are only in our possession. For the first time, cultures were grown in media containing white phosphorus as the sole source of phosphorus. In these environments, microorganisms grew without experiencing phosphorus starvation. That is, they oxidized white phosphorus to phosphate, which is necessary for vital activity! This is first ever example of the inclusion of white phosphorus in the biospheric circulation of the phosphorus element. It turned out that microorganisms that neutralize elemental phosphorus are able to biodegrade most of the spectrum of phosphorus compounds. Our studies of the metabolism of phosphorus-containing compounds of various classes confirm this. Since the chemistry of phosphorus is diverse, it is necessary to collect significant material on the metabolism of many classes of compounds. In this article, we describe the continuation of this work. It turned out that Aspergillus niger AM1 is able to utilize dithiophosphate of the simplest structure as sources of phosphorus, but is not able to utilize substituted dithiophosphonate. In addition, in the present work, we clarified the previously obtained results on the metabolism of phosphoric acid ester and phosphoramide.The NMR method demonstrated that A. niger AM1 slowly metabolizes hypophosphite resulting from the biodegradation of white phosphorus, but does not metabolize phosphite. The NMR data conforms to fungal growth dynamics with these substances in media. Also, was first studied phylogenetic relationship of A. niger AM1 with biodegradable A. niger and A. bombycis strains from the NCBI database.

Download Full-text