Factors affecting the development of disease symptoms in potatoes infected by Tobacco rattle virus

2004 ◽  
Vol 110 (9) ◽  
pp. 921-928 ◽  
Author(s):  
D.J. Robinson ◽  
M.F.B. Dale ◽  
D. Todd
Keyword(s):  
Tobacco Rattle Virus ◽  
Disease Symptoms ◽  
Factors Affecting

scholarly journals Host Range, Biology, and Factors Affecting Survival and Reproduction of the Stubby Root Nematode

1969 ◽  
Vol 54 ◽  
pp. 341-369
Author(s):  
Alejandro Ayala ◽  
M. W. Allen ◽  
E. M. Noffsinger
Keyword(s):  
Plant Species ◽  
Nutrient Solution ◽  
Tobacco Rattle Virus ◽  
Ecological Factors ◽  
Nematode Species ◽  
Coarse Sand ◽  
Nematode Reproduction ◽  
Factors Affecting ◽  
Survival And Reproduction ◽  
Stubby Root Nematode

Populations of T. allius increased to levels nine times higher in Glurk tobacco plants infected with California tobacco rattle virus than in healthy plants of the same variety and age. Populations of T. christiei increased with applications of Hoagland's nutrient solution diluted up to 50-percent concentration, whereas population densities were low at the highest concentration (100 percent), and in water alone. Trichodorus allius did not seem to be affected to a large extent by similar applications of Hoagland's nutrient solution. Soil type influenced populations of T. allius; lighter soils were conducive to development of higher populations while soil, coarse sand, and white quartz sand used separately were not favorable for reproduction. Temperature proved to be one of the most important ecological factors in nematode reproduction. The optimum temperatures for the reproduction of each species was as follows: T. christiei, 16° to 24° C; T. porosus, 24° C; and. T allius, 21° to 24° C. Extremes of temperatures at which the different species could reproduce also varied. Populations of T. christiei were affected by an undetermined disease or condition which seemed to slow movement but did not have any apparent effect on rate of reproduction. Host ranges of three species of Trichodorus were studied. All three nematode species seemed to be polyphagous because 90 percent, 90 percent and 95 percent of the plant species tested were hosts of T. christiei, T. porosus, and T. allius, respectively. Twenty-five plant species were tested for T. porosus, 50 for T. christiei and 38 for T. allius. A method is described for keeping populations of T. allius alive in water for extended periods of time.

scholarly journals A Comparative Study of Two Components of the Poinsettia Root Rot Complex

1971 ◽  
Vol 30 (1-8) ◽  
pp. 419-453
Author(s):  
Robert S. Perry
Keyword(s):  
Environmental Factors ◽  
Comparative Study ◽  
Rhizoctonia Solani ◽  
Root Rot ◽  
Pythium Ultimum ◽  
Thielaviopsis Basicola ◽  
Disease Symptoms ◽  
Factors Affecting

THE MOST IMPORTANT pathological problem encountered by commercial growers of poinsettias ( Euphorbia piilcheirima Willd.) is caused by a complex of fungi composed of Thielaviopsis basicola (Berk. & Br.) Ferr., Rhizoctonia solani Kuhn, and Pythium ultimum Trow. Collectively these three organisms cause a condition referred to as the poinsettia root rot complex. Since more research has been conducted on P. ultimum and R. solani than on T. basicola, the present research was originally designed to investigate some of the environmental factors affecting the growth of Thielaviopsis and the development of the root rot caused by it. However, another fungus, Chalaropsis thielavioides Peyronel, frequently was obtained in isolations from diseased greenhouse poinsettias. Since C. thielavioides had not been reported as being a part of the poinsettia root rot complex, the author decided to investigate its importance as a pathogen on poinsettias. Numerous similarities between C. thielavioides and T. basicola were evident. The object of this work was to compare the two fungi. Prior to undertaking such a study, the pathogenicity of Chalaropsis on poinsettias had to be established. A comparison of Chalaropsis and two isolates of Thielaviopsis was made to determine the effects of environment on the growth of the fungi and the ability of the two fungi to produce disease symptoms on poinsettias.

scholarly journals First report of Gentian Kobu-sho-associated virus infecting peony in the United States and the Netherlands

Plant Disease ◽  
2021 ◽  
Author(s):  
Cullen Shaffer ◽  
David C Michener ◽  
Nastassia Vlasava ◽  
Marleen Botermans ◽  
John Starre ◽  
...  
Keyword(s):  
The Netherlands ◽  
Tobacco Rattle Virus ◽  
The United States ◽  
Causal Agent ◽  
Mottle Virus ◽  
Reference Sequence ◽  
Striking Similarity ◽  
Genome Sequences ◽  
Disease Symptoms ◽  
The Third

Lemoine’s disease of peonies (LDP) is associated with root galls that could lead to stunted growth and reduced flowering. In the quest to identify the causal agent(s) of LDP, two symptomatic plants (cv. Alice Crousse [AC] and Alice Harding [AH]) were sampled in Arkansas in 2019 and sequenced as described (Shaffer et al., 2019). Gentian Kobu-sho-associated virus (GKaV) was present in both plants. The contigs from AH were mapped to the reference sequence of GKaV (AB698918; Kobayashi et al. 2013) yielding 87% of the ~23kb genome, which was completed by Sanger sequencing (Genbank accession no. MW646307) as per Thekke-Veetil et al. (2013). Sample AC was co-infected with cycas necrotic stunt virus (CNSV) and AH with CNSV, citrus leaf blotch virus and lychnis mottle virus. Gentiana triflora -Pall. and G. scabra Bunge plants with Kobu-sho disease symptoms that include galls/tumors on all parts of gentian were also positive for GKaV (Iwadate et al. 2006; Kodama et al. 2004). The striking similarity between symptoms of the two diseases led to the development of a GKaV screening protocol to determine its presence in LPD-affected material. Primers GKaVF 5’-TTAGTGATGAGTGCCTTTTCC-3’ and GKaVR 5’-CTGCCAGTCTTCTTGTGAACC-3’ which amplify a 574 nt region of the virus were used to screen 144 peony leaf samples from the University of Michigan’s Nichols Arboretum collection. Thirty-two (32) plants were stunted whereas 112 displayed normal growth. Nineteen (59%) of the stunted plants tested positive for GKaV compared to eight (6.5%) of the symptomless plants. Partial GKaV genome sequences of three isolates from stunted Michigan plants were deposited in GenBank (MW646310-12) along with three GKaV isolates from Arkansas collected at the same location and time as AC and AH (MW646308-9, MW646313); two had LDP symptoms and the status of the third was unknown. In 2020 four peony root samples from the Netherlands were sequenced as described in Hammond et al. (2021) to identify the causal agent of root galls in three samples. GKaV was present in two: cv. Paul M. Wild and #40391499 and the nearly complete genome sequences were deposited in GenBank (MW916234-5). ‘Paul M. Wild’ was co-infected with cucumber mosaic virus and tobacco rattle virus and #40391499 with a novel amalgavirid. The third symptomatic cv. Many Happy Returns was infected with CNSV while the fourth symptomless cv. Itoh was infected with CNSV and amazon lily mild mottle virus (Shaffer et al., 2021). Percent pairwise identities between sequences were calculated using the SDT Version 1.2 (Muhire et al. 2014). The six partial GKaV sequences from Michigan and Arkansas share 92-100% nt (98-100% aa) identity. Analysis of the three near full length GKaV genomes presented in this communication and the type isolate (NC020252) showed 87-91% nt (93-97% aa) identity. This report provides evidence that GKaV infects peony and is present in Europe and North America. The association of GKaV with LDP is not established, but the virus has been detected in 59% of the plants showing disease symptoms and in ˂7% of asymptomatic plants. We hypothesize that as in the case of Gentian, GKaV has an extended incubation period in peony (Kobayashi et al., 2013) and its titer may fluctuate between seasons as it has been well established for other crops (Villamor et al., 202x). The industry does not perform virus clean-up routinely; propagation material should be tested for GKaV to minimize its spread since the virus may be associated with LDP in at least some cultivars.

Progress In The Practical Application Of Automated Image Analysis To Tem Negatives

1977 ◽  
Vol 35 ◽  
pp. 214-217
Author(s):  
F. A. Heckman ◽  
E. Redman ◽  
J.E. Connolly
Keyword(s):  
Image Analysis ◽  
Image Quality ◽  
Exposure Time ◽  
Image Contrast ◽  
Automated Image Analysis ◽  
Practical Application ◽  
Factors Affecting ◽  
High Image Quality ◽  
Qualitative Evidence ◽  
Comprehensive Study

In our initial publication on this subject1) we reported results demonstrating that contrast is the most important factor in producing the high image quality required for reliable image analysis. We also listed the factors which enhance contrast in order of the experimentally determined magnitude of their effect. The two most powerful factors affecting image contrast attainable with sheet film are beam intensity and KV. At that time we had only qualitative evidence for the ranking of enhancing factors. Later we carried out the densitometric measurements which led to the results outlined below.Meaningful evaluations of the cause-effect relationships among the considerable number of variables in preparing EM negatives depend on doing things in a systematic way, varying only one parameter at a time. Unless otherwise noted, we adhered to the following procedure evolved during our comprehensive study:Philips EM-300; 30μ objective aperature; magnification 7000- 12000X, exposure time 1 second, anti-contamination device operating.

Factors affecting microstructural scale in liquid crystalline polymers

1990 ◽  
Vol 48 (4) ◽  
pp. 220-221
Author(s):  
Christine M. Dannels ◽  
Christopher Viney
Keyword(s):  
Liquid Crystalline ◽  
Liquid Crystalline Polymers ◽  
Factors Affecting ◽  
Crystalline Polymers ◽  
Thermal Expansion Coefficients ◽  
Low Thermal Expansion ◽  
Lower Viscosity ◽  
Fine Microstructure ◽  
Planar Orientation ◽  
Strength And Stiffness

Processing polymers from the liquid crystalline state offers several advantages compared to processing from conventional fluids. These include: better axial strength and stiffness in fibers, better planar orientation in films, lower viscosity during processing, low solidification shrinkage of injection moldings (thermotropic processing), and low thermal expansion coefficients. However, the compressive strength of the solid is disappointing. Previous efforts to improve this property have focussed on synthesizing stiffer molecules. The effect of microstructural scale has been overlooked, even though its relevance to the mechanical and physical properties of more traditional materials is well established. By analogy with the behavior of metals and ceramics, one would expect a fine microstructure (i..e. a high density of orientational defects) to be desirable.Also, because much microstructural detail in liquid crystalline polymers occurs on a scale close to the wavelength of light, light is scattered on passing through these materials.

Factors affecting dentist participation in a state Medicaid program

1990 ◽  
Vol 54 (11) ◽  
pp. 638-643 ◽  
Author(s):  
PC Damiano ◽  
ER Brown ◽  
JD Johnson ◽  
JP Scheetz
Keyword(s):  
Medicaid Program ◽  
Factors Affecting

Speech Therapy Services in a Large School System: A Six-Year Overview

1976 ◽  
Vol 7 (4) ◽  
pp. 207-219 ◽  
Author(s):  
Constance P. DesRoches
Keyword(s):  
School System ◽  
Speech Therapy ◽  
Factors Affecting ◽  
Case Load ◽  
Number Of Children ◽  
School Population ◽  
System A ◽  
Therapy Services ◽  
And Shifts ◽  
Selection Factors

A statistical review provides analysis of four years of speech therapy services of a suburban school system which can be used for comparison with other school system programs. Included are data on the percentages of the school population enrolled in therapy, the categories of disabilities and the number of children in each category, the sex and grade-level distribution of those in therapy, and shifts in case-load selection. Factors affecting changes in case-load profiles are identified and discussed.

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