scholarly journals Correction to: Determination of the protection period of fungicides used for control of Sclerotinia stem rot in soybean through bioassay and chromatography

2021 ◽  
Vol 160 (1) ◽  
pp. 243-243
Author(s):  
Thomas J. J. Miorini ◽  
Carlos Gilberto Raetano ◽  
Matheus M. Negrisoli ◽  
Oscar Pérez-Hernández
2021 ◽  
Author(s):  
Mark C Derbyshire ◽  
Yuphin Khentry ◽  
Anita Severn‐Ellis ◽  
Virginia Mwape ◽  
Nur Shuhadah Mohd Saad ◽  
...  

Author(s):  
Weiguo Dong ◽  
Wenqing Ren ◽  
Xuan Wang ◽  
He Yuke

Abstract MicroRNA319a (miR319a) controls cell division arrest in plant leaves by inhibiting the expression of TCP (TEOSINTE BRANCHED 1/CYCLOIDEA/PCF) family genes. However, it is unclear whether miR319a influences infections by necrotrophic pathogens and host susceptibility. In this study, we revealed that miR319a affected plant resistance to stem rot disease of Sclerotinia sclerotiorum. In the plants of Brassica rapa infected with S. sclerotiorum, miR319a levels increased while expression levels of several BraTCP genes significantly decreased compared with those of the uninfected plants. The overexpression of BraMIR319a in B. rapa increased the susceptibility of the plants to S. sclerotiorum and aggravated stem rot disease, whereas the overexpression of BraTCP4-1 promoted the plant resistance. Our RNA-sequencing data revealed a potential relationship between miR319a and pathogen-related WRKY genes. Chromatin immunoprecipitation (ChIP) assay, electrophoretic mobility shift assay (EMSA) and reporter transaction assay showed that BraTCP4-1 was bound to the promoters of WRKY75, WRKY70, and WRKY33 genes and directly activated these pathogen-related genes. Moreover, the expression levels of WRKY75, WRKY70, and WRKY33 in the plants overexpressing BraMIR319a declined significantly whereas those of the plants overexpressing BraTCP4-1 increased significantly. These results suggest that miR319a and its targeted gene BraTCP4 regulate stem rot resistance through pathways of WRKY genes.


2003 ◽  
Vol 43 (2) ◽  
pp. 163 ◽  
Author(s):  
T. L. Hind ◽  
G. J. Ash ◽  
G. M. Murray

Surveys of petal infestation and stem infection conducted in 1998, 1999 and 2000 indicated that Sclerotinia sclerotiorum poses a threat to the Australian canola industry. Inoculum was present throughout all canola-growing regions of New South Wales and the stem disease was widespread throughout southern New South Wales. Percentage petal infestation increased over the 3 years surveyed with values ranging from 0 to 99.4%. The highest petal infestation values were observed in 2000 (maximum of 99.4%, mean of 82.2%), with lower mean values in 1998 (38.4%) and 1999 (49.6%). Stem infection ranged from 0 to 37.5% and most fields had less than 10% stem infection. Stem rot incidence before harvest did not relate to percentage petal infestation determined during flowering. This indicated that factors other than percentage petal infestation were important in influencing stem rot incidence. While there was no relationship between percentage petal infestation and stem rot incidence, stem infection never occurred without prior petal infestation.


Author(s):  
Fen Gao ◽  
Yuanhong Chen ◽  
SeaRa Lim ◽  
Allen Xue ◽  
Bao-Luo Ma

Effective nitrogen (N) management strategies are important for ensuring a balance between optimizing plant growth and minimizing disease damage. A field experiment was conducted for three years to (i) assess the effects of N fertilizer application on the growth and seed yield of canola, and severities of Sclerotinia stem rot (SSR), and (ii) determine a reasonable N-rate for optimizing plant growth and minimizing the loss from SSR in eastern Canada. The experiment was designed with factorial combinations of eight N treatments and two canola hybrids. All N-treatments reduced canola emergence with increasing preplant N application rates above 100 kg ha–1, but had a positive impact on plant height, fresh weight, dry weight and seed yield. The development of SSR showed differential responses to N application rates. Of all the treatments, the split application (50 kg N ha–1 at preplant plus 100 kg N ha–1 side-dressed at the 6-leaf stage) increased canola growth, and often produced the highest or similar seed yields to those of equivalent N rate applied as preplant. At the 150 kg ha–1 N rate, no severe development of SSR was observed in either preplant-only or split application. Overall, this study demonstrates that the split-N management strategy (50+100 kg ha–1) maintained a balance between enhancing plant growth and mitigating the negative impacts of SSR on canola.


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