Genetic analysis of partial resistance to coffee leaf rust (Hemileia vastatrix Berk & Br.) introgressed into the cultivated Coffea arabica L. from the diploid C. canephora species

Euphytica ◽  
2008 ◽  
Vol 167 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Juan Carlos Herrera P. ◽  
Gabriel Alvarado A. ◽  
Hernando A. Cortina G. ◽  
Marie-Christine Combes ◽  
Gladys Romero G. ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Leaf Rust ◽  
Partial Resistance ◽  
Coffea Arabica ◽  
Hemileia Vastatrix ◽  
Coffee Leaf Rust ◽  
Coffea Arabica L

Partial resistance to leaf rust (Hemileia vastatrix) in coffee (Coffea arabica L.): genetic analysis and molecular characterization of putative candidate genes

2009 ◽  
Vol 25 (4) ◽  
pp. 685-697 ◽  
Author(s):  
Gladys Romero G. ◽  
Gabriel Alvarado A. ◽  
Hernando Cortina G. ◽  
Gustavo Ligarreto M. ◽  
Narmer F. Galeano ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Leaf Rust ◽  
Candidate Genes ◽  
Molecular Characterization ◽  
Partial Resistance ◽  
Coffea Arabica ◽  
Hemileia Vastatrix ◽  
Coffea Arabica L

scholarly journals Ekspresi β -1,3 glukanase dan kitinase pada tanaman kopi arabika (Coffea arabica L.) tahan dan rentan karat daun Expression of β-1,3 glucanase and chitinase of arabica coffee (Coffea arabica L.) resistant and susceptible against leaf rust disease

2016 ◽  
Vol 72 (2) ◽  
Author(s):  
Asmini BUDIANI ◽  
I SUSANTI ◽  
Surip MAWARDI ◽  
D A SANTOSO ◽  
. SISWANTO
Keyword(s):  
Leaf Rust ◽  
Coffea Arabica ◽  
Rt Pcr ◽  
Rust Disease ◽  
Hemileia Vastatrix ◽  
Arabica Coffee ◽  
E Coli ◽  
Genes Encoding ◽  
Pcr Products ◽  
Coffea Arabica L

Summary Leaf rust disease caused by Hemileia vastatrix is considered to be one of the most important diseases on arabica coffee plantation. In order to understand the mechanism underlying resistance of arabica coffee against leaf rust disease, this research was aimed to study expression of β-1,3 glucanase (GLU) and chitinase (CHI) genes in the arabica coffee S1934 and  BLP10 that have been reported respectively as a resistant and susceptible varieties to H. vastatrix. The two varieties were essayed against H. vastatrix, and an RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) using total RNAs  from the S1934 and BLP10, both inoculated with H. vastatrix and uninnoculated was carried out for studying the expression of GLU and CHI. Two primer pairs were designed to amplify the conserved region of GLU and CHI. Amplification products were sequenced and the nucleotide sequences were subjected to BlastX analysis. The result of bioassay confirmed that arabica coffee S1934 was resistant to H. vastatrix, while BLP10 was susceptible.   β-1,3 glucanase was expressed in all of the four samples, the inoculated and uninnoculated S1934, and BLP10 in different degree. S1934 expressed higher GLU compared to BLP10. In the inoculated S1934 the expression of this gene was higher compared to that of the uninoculated one. Expression of CHI was detected only in the S1934, both inoculated and uninoculated. Sequence analysis confirmed that the RT-PCR products were exon regions of genes encoding β-1,3 glucanase dan chitinase respectively. Both of the cDNA fragment have been cloned in E.coli.  Ringkasan Karat daun yang disebabkan oleh jamur Hemileia vastatrix merupakan salah satu penyakit penting pada perkebunan kopi arabika. Untuk memahami mekanisme ketahanan kopi arabika terhadap karat daun, penelitian ini bertujuan untuk mempelajari ekspresi gen β-1,3 glukanase dan kitinase pada varietas kopi arabika S1934 yang dilaporkan tahan karat daun dan varietas BLP10 yang termasuk rentan karat daun. Untuk itu kedua varietas diuji kembali ketahanannya terhadap H. vastatrix melalui bioesai dan dilakukan RT-PCR menggunakan RNA total dari S1934 dan BLP10, baik yang diinokulasi dengan H. vastatrix maupun yang tidak diinokulasi, untuk mempelajari ekspresi gen GLU dan CHI. Dua pasang primer spesifik dirancang untuk mengamplifikasi daerah konservatif kedua gen  tersebut. Hasil amplifikasi disekuen dan dianalisis menggunakan program BlastX. Hasil bioesai mengkonfirmasi bahwa S1934 tahan terhadap H. vastatrix, sedangkan  BLP10 rentan.  β-1,3 glukanase diekspresikan pada kedua varietas, baik yang diinokulasi maupun yang tidak diinokulasi, namun dengan tingkat ekspresi yang sedikit berbeda. Varietas S1934 mengekspresikan β-1,3 glukanase lebih tinggi dibandingkan dengan BLP10. Ekspresi gen tersebut pada S1934 yang diinokulasi lebih tinggi dibandingkan dengan yang tidak diinokulasi. Sedangkan kitinase hanya diekspresikan pada varietas S1934. Hasil sekuensing dan analisis DNA mengkonfirmasi bahwa sekuen hasil RT-PCR merupakan bagian ekson dari gen penyandi β-1,3 glukanase dan kitinase. Kedua fragmen tersebut telah diklon pada E. coli.

scholarly journals Ekspresi β -1,3 glukanase dan kitinase pada tanaman kopi arabika (Coffea arabica L.) tahan dan rentan karat daun Expression of β-1,3 glucanase and chitinase of arabica coffee (Coffea arabica L.) resistant and susceptible against leaf rust disease

2016 ◽  
Vol 72 (2) ◽  
Author(s):  
Asmini BUDIANI ◽  
I SUSANTI ◽  
Surip MAWARDI ◽  
D A SANTOSO ◽  
. SISWANTO
Keyword(s):  
Leaf Rust ◽  
Coffea Arabica ◽  
Rt Pcr ◽  
Rust Disease ◽  
Hemileia Vastatrix ◽  
Arabica Coffee ◽  
E Coli ◽  
Genes Encoding ◽  
Pcr Products ◽  
Coffea Arabica L

Summary Leaf rust disease caused by Hemileia vastatrix is considered to be one of the most important diseases on arabica coffee plantation. In order to understand the mechanism underlying resistance of arabica coffee against leaf rust disease, this research was aimed to study expression of β-1,3 glucanase (GLU) and chitinase (CHI) genes in the arabica coffee S1934 and  BLP10 that have been reported respectively as a resistant and susceptible varieties to H. vastatrix. The two varieties were essayed against H. vastatrix, and an RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) using total RNAs  from the S1934 and BLP10, both inoculated with H. vastatrix and uninnoculated was carried out for studying the expression of GLU and CHI. Two primer pairs were designed to amplify the conserved region of GLU and CHI. Amplification products were sequenced and the nucleotide sequences were subjected to BlastX analysis. The result of bioassay confirmed that arabica coffee S1934 was resistant to H. vastatrix, while BLP10 was susceptible.   β-1,3 glucanase was expressed in all of the four samples, the inoculated and uninnoculated S1934, and BLP10 in different degree. S1934 expressed higher GLU compared to BLP10. In the inoculated S1934 the expression of this gene was higher compared to that of the uninoculated one. Expression of CHI was detected only in the S1934, both inoculated and uninoculated. Sequence analysis confirmed that the RT-PCR products were exon regions of genes encoding β-1,3 glucanase dan chitinase respectively. Both of the cDNA fragment have been cloned in E.coli.  Ringkasan Karat daun yang disebabkan oleh jamur Hemileia vastatrix merupakan salah satu penyakit penting pada perkebunan kopi arabika. Untuk memahami mekanisme ketahanan kopi arabika terhadap karat daun, penelitian ini bertujuan untuk mempelajari ekspresi gen β-1,3 glukanase dan kitinase pada varietas kopi arabika S1934 yang dilaporkan tahan karat daun dan varietas BLP10 yang termasuk rentan karat daun. Untuk itu kedua varietas diuji kembali ketahanannya terhadap H. vastatrix melalui bioesai dan dilakukan RT-PCR menggunakan RNA total dari S1934 dan BLP10, baik yang diinokulasi dengan H. vastatrix maupun yang tidak diinokulasi, untuk mempelajari ekspresi gen GLU dan CHI. Dua pasang primer spesifik dirancang untuk mengamplifikasi daerah konservatif kedua gen  tersebut. Hasil amplifikasi disekuen dan dianalisis menggunakan program BlastX. Hasil bioesai mengkonfirmasi bahwa S1934 tahan terhadap H. vastatrix, sedangkan  BLP10 rentan.  β-1,3 glukanase diekspresikan pada kedua varietas, baik yang diinokulasi maupun yang tidak diinokulasi, namun dengan tingkat ekspresi yang sedikit berbeda. Varietas S1934 mengekspresikan β-1,3 glukanase lebih tinggi dibandingkan dengan BLP10. Ekspresi gen tersebut pada S1934 yang diinokulasi lebih tinggi dibandingkan dengan yang tidak diinokulasi. Sedangkan kitinase hanya diekspresikan pada varietas S1934. Hasil sekuensing dan analisis DNA mengkonfirmasi bahwa sekuen hasil RT-PCR merupakan bagian ekson dari gen penyandi β-1,3 glukanase dan kitinase. Kedua fragmen tersebut telah diklon pada E. coli.

Hemileia vastatrix. [Descriptions of Fungi and Bacteria].

1964 ◽  
Author(s):  
G. F. Laundon
Keyword(s):  
Leaf Rust ◽  
Geographical Distribution ◽  
Long Range ◽  
Coffea Arabica ◽  
Lower Surface ◽  
Spore Dispersal ◽  
Hemileia Vastatrix ◽  
Coffee Leaf Rust ◽  
Rain Splash ◽  
Yellowish Orange

Abstract A description is provided for Hemileia vastatrix. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On Coffea arabica, C. canephora, C. liberica, and other Coffea spp. DISEASE: Coffee leaf rust. Produces yellowish-orange, powdery, rounded blotches on the lower surface of the leaves, which may coalesce with others to form an irregularly shaped lesion, accompanied by a chlorosis of the upper surface. With age, the centre of the leaf turns dark brown and dies, followed by premature defoliation and die-back of the branches. Has also on rare occasions been recorded on berries and young shoots. GEOGRAPHICAL DISTRIBUTION: Africa, Asia, Australasia, end Oceania (CMI Map 5). TRANSMISSION: Earlier workers [Ward, J. Linn. Soc. (Bot.) 19: 299-335, 1882, Mayne (12: 285)] attributed spore dispersal to wind but more recent investigations have emphasised the role played by rain-splash over short distances (Bock, Trans. Brit. Mycol. Soc. 45: 289-300, 1962). Air currents may be implicated in long-range inter-continental dispersal (Wellman, 1957). Two species of thrips have been found feeding on and aiding the dispersal of urediospores in India (40: 467) and urediospores have also been observed to be mechanically transported on the bodies of two species of hymenopterous parasites of larvae of cecidomyid midges commonly found feeding on urediospores in Kenya (Crowe, Trans. Brit. Mycol. Soc. 46: 24-26, 1963).

Development of sequence characterized DNA markers linked to leaf rust (Hemileia vastatrix) resistance in coffee (Coffea arabica L.)

2007 ◽  
Vol 21 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Laetitia Mahé ◽  
Marie-Christine Combes ◽  
Vitor M. P. Várzea ◽  
Claire Guilhaumon ◽  
Philippe Lashermes
Keyword(s):  
Leaf Rust ◽  
Dna Markers ◽  
Coffea Arabica ◽  
Hemileia Vastatrix ◽  
Coffea Arabica L

Timing and Frequency of Spraying for Control of Coffee Leaf Rust in Southern India

1969 ◽  
Vol 5 (2) ◽  
pp. 117-123 ◽  
Author(s):  
K. C. Ananth
Keyword(s):  
Leaf Rust ◽  
Leaf Spot ◽  
Coffea Arabica ◽  
Hemileia Vastatrix ◽  
Early Season ◽  
Leaf Disease ◽  
Coffee Leaf Rust ◽  
Mysore State ◽  
Hot Weather ◽  
Coorg District

SummaryTo study control of Hemileia vastatrix leaf disease in Coffea arabica L., an experiment was run for a year in Coorg District of Mysore State. Three and four early-season sprays coupled with two mid-season ones gave a reasonable control, as evaluated by leaf spot counts, but a high proportion of rust-affected leaves were retained on the bushes. Infection commenced in the hot weather, immediately after showers, and the number of infected leaves appears to have been more important than the average leaf spot counts. Control of leaf disease up to December, and a late incidence of the rust during January, may not affect crop prospects appreciably. It is suggested that reduced early sprays coupled with pre-monsoon and timely mid-season schedules should reasonably control the incidence of rust.

The fine structure of the coffee leaf rust, Hemileia vastatrix

1974 ◽  
Vol 52 (6) ◽  
pp. 1363-1367 ◽  
Author(s):  
Luisete Rijo ◽  
J. A. Sargent
Keyword(s):  
Fine Structure ◽  
Golgi Apparatus ◽  
Leaf Rust ◽  
Wall Thickness ◽  
Coffea Arabica ◽  
Rust Fungi ◽  
The Body ◽  
Hemileia Vastatrix ◽  
Coffee Leaf Rust

The fine structure of two races of Hemileia vastatrix that cause leaf rust in Coffea arabica is similar in many respects to earlier descriptions of other rust fungi. Certain features, however, are distinctive. Both hyphae and haustoria contain occasional tubular complexes composed of tubules about 300 Å in diameter. These might function as a Golgi apparatus. The haustorium neck ring incorporates the entire wall thickness at its proximal end, but distally only the outer and innermost layers of the wall are involved. The invaginated host plasmalemma, which surrounds the body of the haustorium, has a furrowed surface and in the adjacent haustorial sheath fibrillar elements are prominent. The plasmalemma furrows and the sheath fibrillar elements are discussed in relation to the organization of the structure of the sheath and absorption of materials through it.

scholarly journals A fungus-eat-fungus world: Digitopodium, with particular reference to mycoparasites of the coffee leaf rust, Hemileia vastatrix

IMA Fungus ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Adans A. Colmán ◽  
Harry C. Evans ◽  
Sara S. Salcedo-Sarmiento ◽  
Uwe Braun ◽  
Kifle Belachew-Bekele ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Democratic Republic Of Congo ◽  
Sequence Data ◽  
Molecular Study ◽  
Classical Biological Control ◽  
Coffea Canephora ◽  
Molecular Data ◽  
Phylogenetic Position ◽  
Hemileia Vastatrix ◽  
Coffee Leaf Rust

AbstractDigitopodium hemileiae was described originally in 1930 as Cladosporium hemileiae; growing as a mycoparasite of the coffee leaf rust (CLR), Hemileia vastatrix, in a sample of diseased leaves of Coffea canephora collected in the Democratic Republic of Congo. No cultures from this material exist. More recently, the type material was re-examined and, based on morphological features, considered to be incorrectly placed in Cladosporium. The new genus Digitopodium was erected to accommodate this species. Interest in fungal antagonists of H. vastarix, as potential biocontrol agents of CLR, led to comprehensive surveys for mycoparasites, both in the African centre of origin of the rust, as well as in its South American exotic range. Among the rust specimens from Ethiopia, one was found to be colonized by a fungus congeneric with, and similar to, D. hemileiae. Pure cultures obtained from the Ethiopian material enabled a molecular study and for its phylogenetic position to be elucidated, based on DNA sequence data from the ITS and LSU regions. Molecular data showed that two members of the recently erected genus Hyalocladosporiella (Herpotrichiellaceae: Chaetothyriales) are congeneric with Digitopodium from Ethiopia and morphologically similar to both D. hemileiae and the two Ethiopian isolates. These isolates were found to be morphologically and genetically identical to H. tectonae, described previously from Brazil. Thus, species of Hyalocladosporiella are re-allocated to Digitopodium here; including D. tectonae, and a novel species, D. canescens, recently found in Brazil growing as a mycoparasite of Puccinia thaliae. The potential use of D. hemileiae and D. tectonae for classical biological control of CLR is discussed.

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