S1 satellite DNA repetitive units display identical structure and overall variability in all Anatolian brown frog taxa

Digestion of Rana graeca italica DNA with Asp718I produces highly repetitive fragments of 281 and 385 bp that were cloned and sequenced. The shorter fragment corresponds to the unit repeat (RgiS1b) of a satellite DNA. The longer fragment was found to be part of a 494-bp repeat of another satellite DNA (RgiS1a) that was cloned intact as an EcoRV fragment. RgiS1b is 97% homologous to RgiS1a, from which it seems to be derived by a single deletion. Among all species tested, only the related brown frog Rana dalmatina contained homologous repetitive DNA. The overall number of RgiS1a and RgiS1b repeats per R. graeca italica haploid genome was estimated to be 2.7 × 105. RgiS1a and RgiS1b repeats are organized in separate arrays, but repetitive units formed by various combinations of the two repeats were also observed on Southern blots. The amount of these extra repeats varies greatly among animals from the same population, representing a rare case of individual variability in the satellite DNA organization. FISH with probes specific for both satellites, or for RgiS1a only, labeled the centromeric and pericentromeric heterochromatin of all chromosomes. This indicated that RgiS1a and RgiS1b are interspersed within the same heterochromatic regions of the chromosomes.Key words: satellite DNA, nucleotide sequence analysis, tandem repeats organization, amphibian chromosomes.

Download Full-text

Faculty Opinions recommendation of Hairpin structures formed by alpha satellite DNA of human centromeres are cleaved by human topoisomerase IIalpha.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1123281.580428 ◽

2008 ◽

Author(s):

Pat Heslop-Harrison

Keyword(s):

Satellite Dna ◽

Alpha Satellite ◽

Alpha Satellite Dna ◽

Topoisomerase Iialpha ◽

Hairpin Structures

Download Full-text

satDNA Analyzer 1.2 as a Valuable Computing Tool for Evolutionary Analysis of Satellite-DNA Families: Revisiting Y-Linked Satellite-DNA Sequences of Rumex (Polygonaceae)

Bioinformatics Research and Development - Lecture Notes in Computer Science ◽

10.1007/978-3-540-71233-6_11 ◽

2007 ◽

pp. 131-139

Author(s):

Rafael Navajas-Pérez ◽

Manuel Ruiz Rejón ◽

Manuel Garrido-Ramos ◽

José Luis Aznarte ◽

Cristina Rubio-Escudero

Keyword(s):

Dna Sequences ◽

Satellite Dna ◽

Evolutionary Analysis

Download Full-text

Evolution of Sex Chromosome Heteromorphy in Geographic Populations of the Japanese Tago’s Brown Frog Complex

Cytogenetic and Genome Research ◽

10.1159/000512964 ◽

2021 ◽

pp. 1-9

Author(s):

Chiao Kuwana ◽

Hiroyuki Fujita ◽

Masataka Tagami ◽

Takanori Matsuo ◽

Ikuo Miura

Keyword(s):

Sex Chromosomes ◽

Sex Chromosome ◽

Mitochondrial Gene ◽

Population Level ◽

Chromosome 7 ◽

Geographic Population ◽

Geographic Populations ◽

Western Japan ◽

Brown Frog ◽

Eastern Japan

The sex chromosomes of most anuran amphibians are characterized by homomorphy in both sexes, and evolution to heteromorphy rarely occurs at the species or geographic population level. Here, we report sex chromosome heteromorphy in geographic populations of the Japanese Tago’s brown frog complex (2n = 26), comprising Rana sakuraii and R. tagoi. The sex chromosomes of R. sakuraii from the populations in western Japan were homomorphic in both sexes, whereas chromosome 7 from the populations in eastern Japan were heteromorphic in males. Chromosome 7 of R. tagoi, which is distributed close to R. sakuraii in eastern Japan, was highly similar in morphology to the Y chromosome of R. sakuraii. Based on this and on mitochondrial gene sequence analysis, we hypothesize that in the R. sakuraii populations from eastern Japan the XY heteromorphic sex chromosome system was established by the introduction of chromosome 7 from R. tagoi via interspecies hybridization. In contrast, chromosome 13 of R. tagoi from the 2 large islands in western Japan, Shikoku and Kyushu, showed a heteromorphic pattern of constitutive heterochromatin distribution in males, while this pattern was homomorphic in females. Our study reveals that sex chromosome heteromorphy evolved independently at the geographic lineage level in this species complex.

Download Full-text

Evolution of a Multiple Sex-Chromosome System by Three-Sequential Translocations among Potential Sex-Chromosomes in the Taiwanese Frog Odorrana swinhoana

Cells ◽

10.3390/cells10030661 ◽

2021 ◽

Vol 10 (3) ◽

pp. 661

Author(s):

Ikuo Miura ◽

Foyez Shams ◽

Si-Min Lin ◽

Marcelo de Bello Cioffi ◽

Thomas Liehr ◽

...

Keyword(s):

Sex Chromosomes ◽

Rare Case ◽

Sex Chromosome ◽

Sex Chromosome System ◽

Brown Frog ◽

Male Specific

Translocation between sex-chromosomes and autosomes generates multiple sex-chromosome systems. It happens unexpectedly, and therefore, the evolutionary meaning is not clear. The current study shows a multiple sex chromosome system comprising three different chromosome pairs in a Taiwanese brown frog (Odorrana swinhoana). The male-specific three translocations created a system of six sex-chromosomes, ♂X1Y1X2Y2X3Y3 -♀X1X1X2X2X3X3. It is unique in that the translocations occurred among three out of the six members of potential sex-determining chromosomes, which are known to be involved in sex-chromosome turnover in frogs, and the two out of three include orthologs of the sex-determining genes in mammals, birds and fishes. This rare case suggests sex-specific, nonrandom translocations and thus provides a new viewpoint for the evolutionary meaning of the multiple sex chromosome system.

Download Full-text

Satellite DNA probes of Alstroemeria longistaminea (Alstroemeriaceae) paint the heterochromatin and the B chromosome, reveal a G-like banding pattern, and point to a strong structural karyotype conservation

PROTOPLASMA ◽

10.1007/s00709-021-01681-7 ◽

2021 ◽

Author(s):

Tiago Ribeiro ◽

Magdalena Vaio ◽

Leonardo P. Félix ◽

Marcelo Guerra

Keyword(s):

Banding Pattern ◽

Satellite Dna ◽

B Chromosome ◽

Dna Probes

Download Full-text

Genomic Tackling of Human Satellite DNA: Breaking Barriers through Time

International Journal of Molecular Sciences ◽

10.3390/ijms22094707 ◽

2021 ◽

Vol 22 (9) ◽

pp. 4707

Author(s):

Mariana Lopes ◽

Sandra Louzada ◽

Margarida Gama-Carvalho ◽

Raquel Chaves

Keyword(s):

Human Genome ◽

Satellite Dna ◽

Repetitive Sequences ◽

Nucleotide Composition ◽

Genomic Component ◽

Genomic Studies ◽

Human Genomic ◽

Definition Of ◽

High Degree

(Peri)centromeric repetitive sequences and, more specifically, satellite DNA (satDNA) sequences, constitute a major human genomic component. SatDNA sequences can vary on a large number of features, including nucleotide composition, complexity, and abundance. Several satDNA families have been identified and characterized in the human genome through time, albeit at different speeds. Human satDNA families present a high degree of sub-variability, leading to the definition of various subfamilies with different organization and clustered localization. Evolution of satDNA analysis has enabled the progressive characterization of satDNA features. Despite recent advances in the sequencing of centromeric arrays, comprehensive genomic studies to assess their variability are still required to provide accurate and proportional representation of satDNA (peri)centromeric/acrocentric short arm sequences. Approaches combining multiple techniques have been successfully applied and seem to be the path to follow for generating integrated knowledge in the promising field of human satDNA biology.

Download Full-text