Optimization of Recombination in Interspecific Hybrids to Introduce New Genetic Diversity into Oilseed Rape (Brassica napus L.)

The genetic diversity of 94 accessions of winter oilseed rape (Brassica napus L.), representing past and contemporary material utilized in the Czech breeding programmes, was determined using microsatellites (SSRs), ISSRs and AFLPs. All three kinds of markers differed in the range of observed polymorphism and differentiated clearly each accession. Altogether 89 SSR, 1003 AFLP and 53 ISSR markers were evaluated. Their average rates of polymorphic bands were 100%, 53.9% and 90.6%, respectively, indicating high genetic diversity among the accessions. The greatest genetic distance was found by ISSRs (62.3%) whereas significantly lower distances of 49.4% in SSRs and 35.5% in AFLPs were observed. The genetic similarity matrix clearly distinguished all accessions. A set of the most distinct varieties was established. The analysis of the genetic pattern of the accessions indicated two groups comprising most of the modern Czech breeding materials, revealing a distinct shift in breeding. Surprisingly, molecular analyses did not support  breeders’ views about a narrow genetic base of the Czech breeding materials. The choice of appropriate technology for different aspects of germplasm evaluation is also discussed.

Download Full-text

Polymorphism of microsatellite loci in feral populations and commercial varieties of oilseed rape (Brassica napus L.)

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v25.1141 ◽

2019 ◽

Vol 25 ◽

pp. 67-73

Author(s):

V. A. Lemesh ◽

M Bahdanava ◽

G. Mozgova ◽

A. Burakova

Keyword(s):

Genetic Diversity ◽

Brassica Napus ◽

Oilseed Rape ◽

Environmental Risk ◽

Microsatellite Loci ◽

Genetic Divergence ◽

Brassica Napus L ◽

Feral Populations ◽

Transgenic Rape ◽

Transgenic Rapeseed

Aim. The aim of the work was to compare the polymorphism of commercial varieties and populations of B. napus growing outside cultivation to assess the genetic diversity of feral rapeseed populations in Belarus. Methods. The study assessed genetic diversity according to the data of 7 microsatellite loci genotyping. Results. The results indicate a greater genetic diversity in feral oilseed rape populations. An analysis of the structure of the genotypes distribution in the STRUCTURE software showed the division into three clusters – commertial varieties, feral populations and samples of B. rapa. Conclusions. The established genetic divergence between feral populations and commercial varieties indicates that feral oilseed rape is able to maintain persistent populations in Belarus. In practice, this should be taken into account when assessing the environmental risk when transgenic rape is released into the environment. And in the cultivation of transgenic rapeseed, special attention should be paid to measures to prevent the occurrence of its free-growing populations. Keywords: oilseed rape, feral populations, microsatellite loci, genetic diversity.

Download Full-text

Genetic divergence between feral populations of rape and varieties Brassica napus L.

Doklady of the National Academy of Sciences of Belarus ◽

10.29235/1561-8323-2019-63-4-466-475 ◽

2019 ◽

Vol 63 (4) ◽

pp. 466-475

Author(s):

Valiantsina A. Lemesh ◽

Maryna V. Bahdanava ◽

Galina V. Mozgova ◽

Aryna A. Burakova ◽

Mikalai E. Kharuzhy

Keyword(s):

Genetic Diversity ◽

Brassica Napus ◽

Oilseed Rape ◽

Genetic Divergence ◽

Hybrid Origin ◽

Genotype Distribution ◽

Brassica Napus L ◽

Genetic Characteristics ◽

Feral Populations ◽

The Republic

The study assessed the genetic diversity of commercial varieties and feral populations of oilseed rape (Brassica napus L.) grown in the Republic of Belarus according to the genotyping data of 7 microsatellite loci – Na12D08, Ol12D04-1, Ol12D04-2, Ra2A05, Na10H03, Na14H11, Ol11B05. The following parameters were calculated: the average number of alleles per locus, the eﬀective number of alleles, the levels of expected and observed heterozygosity, % of polymorphic loci. The results indicate a greater genetic diversity in feral oilseed rape populations. According to the cluster analysis, one feral population fell into a cluster of commercial varieties and demonstrated similarities with the variety Atora, which indicates its recent wildness and the preservation of the genetic characteristics inherent in cultivated varieties. The variety Mercedes was clustered together with samples of feral populations, which may indicate its hybrid origin. An analysis of the structure of the genotype distribution in the Structure software showed the division into three clusters – commercial varieties, feral populations and samples of B. rapa. The established genetic divergence between feral populations and commercial varieties indicates that feral oilseed rape is able to maintain persistent populations inBelarus. In practice, this should be taken into account while assessing the environmental risk when transgenic rape is released into the environment. When cultivating transgenic rapeseed, special attention should be paid to measures to prevent the occurrence of its free-growing populations.

Download Full-text

Morphological traits and genetic diversity of differently overwintered oilseed rape (Brassica napus L.) cultivars

Zemdirbyste-Agriculture ◽

10.13080/z-a.2013.100.052 ◽

2013 ◽

Vol 100 (4) ◽

pp. 409-416 ◽

Cited By ~ 4

Author(s):

Algimantas Paulauskas ◽

Milda Jodinskienė ◽

Loreta Griciuvienė ◽

Judita Žukauskienė ◽

Eglė Petraitienė ◽

...

Keyword(s):

Genetic Diversity ◽

Brassica Napus ◽

Oilseed Rape ◽

Morphological Traits ◽

Brassica Napus L

Download Full-text

Seed Yield and Sulphur Partitioning in Two Inbred Lines of Low and High Glucosinolate Oilseed Rape (Brassica napus L.) and Their Hybrids at Three Levels of Sulphur Supply

Acta Agriculturae Scandinavica Section B - Soil & Plant Science ◽

10.1080/09064719908565565 ◽

1999 ◽

Vol 49 (3) ◽

pp. 184-188

Author(s):

J. Davik ◽

A. K. Bakken

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Seed Yield ◽

Inbred Lines ◽

Brassica Napus L

Download Full-text

Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape (Brassica napus L.) against Fungal Pathogens

International Journal of Molecular Sciences ◽

10.3390/ijms21228740 ◽

2020 ◽

Vol 21 (22) ◽

pp. 8740

Author(s):

Daria Chlebek ◽

Artur Pinski ◽

Joanna Żur ◽

Justyna Michalska ◽

Katarzyna Hupert-Kocurek

Keyword(s):

Brassica Napus ◽

Plant Growth ◽

Pseudomonas Fluorescens ◽

Oilseed Rape ◽

Plant Growth Promotion ◽

Fungal Pathogens ◽

Growth Promotion ◽

Genome Mining ◽

Plant Colonization ◽

Brassica Napus L

Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of Pseudomonas fluorescens BRZ63, a new endophyte of oilseed rape (Brassica napus L.) against Rhizoctonia solani W70, Colletotrichum dematium K, Sclerotinia sclerotiorum K2291, and Fusarium avenaceum. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of Arabidopsis thaliana Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion.

Download Full-text