scholarly journals Inter-laboratory validation of the serial dilution culture—most probable number method for enumerating viable phytoplankton

2018 ◽  
Vol 31 (1) ◽  
pp. 491-503
Author(s):  
Hugh L. MacIntyre ◽  
John J. Cullen ◽  
Shannah Rastin ◽  
Magdalena Waclawik ◽  
Kimberly J. Franklin ◽  
...  
Keyword(s):  
Serial Dilution ◽  
Most Probable Number ◽  
Probable Number ◽  
Dilution Culture ◽  
Serial Dilution Culture

scholarly journals Liquid Serial Dilution Is Inferior to Solid Media for Isolation of Cultures Representative of the Phylum-Level Diversity of Soil Bacteria

2004 ◽  
Vol 70 (7) ◽  
pp. 4363-4366 ◽  
Author(s):  
Liesbeth Schoenborn ◽  
Penelope S. Yates ◽  
Bronwyn E. Grinton ◽  
Philip Hugenholtz ◽  
Peter H. Janssen
Keyword(s):  
Soil Bacteria ◽  
Serial Dilution ◽  
Bacterial Phyla ◽  
Pasture Soil ◽  
Solid Media ◽  
Dilution Culture ◽  
Serial Dilution Culture ◽  
Bacterial Lineages

ABSTRACT Representatives of only four well-characterized bacterial phyla were isolated from a pasture soil by using liquid serial dilution culture. In contrast, members of Acidobacteria, Verrucomicrobia, and Gemmatimonadetes and of other poorly represented bacterial lineages were isolated in earlier experiments with solidified versions of the same media. We conclude that, contrary to expectation, liquid serial dilution culture is inferior to culturing on solid media for isolating representatives of many bacterial phyla from soil.

scholarly journals Nutrient levels and trade-offs control diversity in a serial dilution ecosystem

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Amir Erez ◽  
Jaime G Lopez ◽  
Benjamin G Weiner ◽  
Yigal Meir ◽  
Ned S Wingreen
Keyword(s):  
Resource Competition ◽  
Nutrient Supply ◽  
Serial Dilution ◽  
Community Diversity ◽  
Ecosystem Stability ◽  
Early Bird ◽  
Trade Offs ◽  
Dilution Culture ◽  
Serial Dilution Culture ◽  
High Diversity

Microbial communities feature an immense diversity of species and this diversity is linked to outcomes ranging from ecosystem stability to medical prognoses. Yet the mechanisms underlying microbial diversity are under debate. While simple resource-competition models don't allow for coexistence of a large number of species, it was recently shown that metabolic trade-offs can allow unlimited diversity. Does this diversity persist with more realistic, intermittent nutrient supply? Here, we demonstrate theoretically that in serial dilution culture, metabolic trade-offs allow for high diversity. When a small amount of nutrient is supplied to each batch, the serial dilution dynamics mimic a chemostat-like steady state. If more nutrient is supplied, community diversity shifts due to an 'early-bird' effect. The interplay of this effect with different environmental factors and diversity-supporting mechanisms leads to a variety of relationships between nutrient supply and diversity, suggesting that real ecosystems may not obey a universal nutrient-diversity relationship.

Analysis of Coliform and Colifecal Total Pollution Test on Various Types of Drinking Water Using the MPN (Most Probable Number) Method

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Wanda Aulya ◽  
Fadhliani Fadhliani ◽  
Vivi Mardina
Keyword(s):  
Drinking Water ◽  
Fecal Coliform ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Inspection Method ◽  
Sample Number ◽  
Probable Number ◽  
Fecal Coliform Bacteria

Water is the main source for life and also the most severe substance caused by pollution. The mandatory parameters for determining microbiological quality of drinking water are total non-fecal Coliform bacteria and Coliform fecal (Escherichia coli). Coliform bacteria are a group of microorganisms commonly used as indicators, where these bacteria can be a signal to determine whether a water source has been contaminated by bacteria or not, while fecal Coliform bacteria are indicator bacteria polluting pathogenic bacteria originating from human feces and warm-blooded animals (mammals) . The water inspection method in this study uses the MPN (Most Probable Number) method which consists of 3 tests, namely, the presumption test, the affirmation test, and the reinforcement test. The results showed that of 15 drinking water samples 8 samples were tested positive for Coliform bacteria with the highest total bacterial value of sample number 1, 15 (210/100 ml), while 7 other samples were negative. From 8 positive Coliform samples only 1 sample was stated to be negative fecal Coliform bacteria and 7 other samples were positive for Coliform fecal bacteria with the highest total bacterial value of sample number 1 (210/100 ml).

Cemaran Eshericia coli dalam makanan laut yang beredar di pasar tradisional Kota Pontian

2015 ◽  
Vol 1 (1) ◽  
pp. 44
Author(s):  
Rafika Sari ◽  
Pratiwi Apridamayanti
Keyword(s):  
Most Probable Number ◽  
Probable Number

Latar Belakang: Makanan laut merupakan salah satu jenis makanan yang banyak dikonsumsi oleh masyarakat selain sebagai komoditi ekspor. Mengkonsumsi makanan laut yang telah terkontaminasi bakteri hidup atau toksin yang dihasilkannya dapat menyebabkan keracunan makanan. Tujuan penelitian ini adalah untuk mengetahui adanya kontaminasi bakteri koliform E.coli sebagai indikator pencemaran pada makanan laut dan memberikan informasi kelayakan dan keamanan konsumsi dari makanan laut di dua pasar tradisional terbesar di daerah Pontianak. Metode: Sampel yang digunakan adalah ikan, sotong dan udang. Penelitian terhadap sampel dilakukan menggunakan uji Most Probable Number (MPN) yang dilengkapi dengan uji biokimia untuk mengidentifikasi jenis bakteri pada sampel melalui penanaman bakteri pada media agar Lactose Broth (LB) dan Briliant Green Lactose Bile Broth (BGLB). Hasil: Hasil penelitian menunjukkan bakteri koliform E.coli terdeteksi pada 100% sampel dengan nilai MPN yang tidak memenuhi kriteria kelayakan konsumsi, yakni >3/g. Kesimpulan: Makanan yang ada tidak memenuhi kriteria kelayakan konsumsi.

Sign in / Sign up

Export Citation Format

Share Document