Analysis of Coliform and Colifecal Total Pollution Test on Various Types of Drinking Water Using the MPN (Most Probable Number) Method

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Wanda Aulya ◽  
Fadhliani Fadhliani ◽  
Vivi Mardina
Keyword(s):  
Drinking Water ◽  
Fecal Coliform ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Inspection Method ◽  
Sample Number ◽  
Probable Number ◽  
Fecal Coliform Bacteria

Water is the main source for life and also the most severe substance caused by pollution. The mandatory parameters for determining microbiological quality of drinking water are total non-fecal Coliform bacteria and Coliform fecal (Escherichia coli). Coliform bacteria are a group of microorganisms commonly used as indicators, where these bacteria can be a signal to determine whether a water source has been contaminated by bacteria or not, while fecal Coliform bacteria are indicator bacteria polluting pathogenic bacteria originating from human feces and warm-blooded animals (mammals) . The water inspection method in this study uses the MPN (Most Probable Number) method which consists of 3 tests, namely, the presumption test, the affirmation test, and the reinforcement test. The results showed that of 15 drinking water samples 8 samples were tested positive for Coliform bacteria with the highest total bacterial value of sample number 1, 15 (210/100 ml), while 7 other samples were negative. From 8 positive Coliform samples only 1 sample was stated to be negative fecal Coliform bacteria and 7 other samples were positive for Coliform fecal bacteria with the highest total bacterial value of sample number 1 (210/100 ml).

scholarly journals Bacteria Identification In Traditional Sauce (Sambal) At The Restaurants Nearby The Traditional Markei Of Gorontalo City

2021 ◽  
Vol 2 (1) ◽  
pp. 11-18
Author(s):  
Ana Pepiana ◽  
Laksmyn Kadir ◽  
Agusrianto Yusuf
Keyword(s):  
Fecal Coliform ◽  
Sampling Technique ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Fecal Coliform Bacteria ◽  
Negative Results ◽  
Escherchia Coli ◽  
Traditional Market ◽  
Positive Results

The unsafe food will cause the health problems in the community. Sambal which is known as the traditional sauce is a sauce made from chilies that are crushed until the water content comes out, giving it a spicy taste. Furthermore, Sambal is processed in a simple way so that it can leac to the contamination of microorganisms such as Coliform bacteria contamination. This study aims to determine the presence of Coliform bacteria contamination in Sambal at the supermarket nearby the traditional market in Gorontalo City. This research is a descriptive study using a qualitative approach. It examines the existence of Coliform bacteria using the Most Probable Number (MPN) method. The population in this study were 14 samples with the sampling technique by accidental sampling. Coliform bacteria examination results show that Sambal sample is found with positive results contaminated with Coliform bacteria, namely 11 samples with a percentage of 78.6% and negative results of 3 samples with a percentage of 21.4%. Based on the results, it can be concluded that a positive result was found consisting of 1 sample of coliform fekal bacteria, in this case the bakteri Escherchia coli. bacteria, and 10 samples from the non-fecal coliform bacteria.

scholarly journals The origin of bacterial contamination in AL-Habania reservoir in Iraq

2011 ◽  
Vol 8 (2) ◽  
pp. 243-247
Author(s):  
Baghdad Science Journal
Keyword(s):  
Fecal Coliform ◽  
Bacterial Contamination ◽  
Fecal Coliforms ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Faecal Coliforms ◽  
Probable Number ◽  
Fecal Coliform Bacteria ◽  
Fecal Streptococci ◽  
Faecal Streptococci

Bacterial contamination of AL-Habania reservoir was studied during the period from February 2005 to January 2006; samples were collected from four stations (AL-Warrar, AL-Theban regulator, middle of the reservoir and the fourth was towards AL-Razzaza reservoir). Coliform bacteria, faecal Coliforms, Streptococci, and faecal Streptococci were used as parameters of bacterial contamination in waters through calculating the most probable number. Highest count of Coliform bacteria (1500 cell/100ml) was recorded at AL-Razaza during August, and the lowest count was less than (300 cell/100ml) in the rest of the collection stations for all months. Fecal Coliform bacteria ranged between less than 300 cells/100ml in all stations for all months to 700 cell/100ml in AL-Warrar, AL-Razaza and in the middle of the reservoir stations during August. Streptococci bacteria count ranged between less than 300 cell/100ml to 700 cell/100ml as a highest record in AL-Razaza station during August for both. The ratio between fecal coliforms and fecal streptococci (FC: FS) was detected to determined the origin of the pollution in the reservoir depending on Geldrich statistical law in this research, the ratio ranged between (1) to (2.3).

scholarly journals Escherichia coli O157:H7 in Drin Prevalence of Escherichia coli O157:H7 in Drink from Different Food Premises in Kota Samarahan Sarawak

2019 ◽  
Vol 2 (2) ◽  
pp. a13-19
Author(s):  
ELEXSON NILLIAN ◽  
AMIZA NUR ◽  
DIYANA NUR ◽  
AMIRAH ZAKIRAH ◽  
GRACE BEBEY
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Plain Water ◽  
Escherichia Coli O157 ◽  
Probable Number ◽  
E Coli ◽  
Preventive Actions ◽  
Stx1 Gene

Contamination of drinks with E. coli O157:H7 served in food premises such as restaurants can cause haemorrhagic colitis and haemolytic uremic syndrome to humans. The presence or absence of faecal pathogen was demonstrated using coliform group as indicator microorganisms. Therefore, this study was conducted to detect the presence of E. coli O157:H7 in drinking water from food restaurant premise in Kota Samarahan and Kuching to ensure safe and potable drinking water is served to the consumer. A total of thirty (n=30) drink samples including six types of each of the samples are cold plain water, iced tea, iced milo, syrup and iced milk tea. Most Probable Number (MPN) procedure was used in this study to enumerate the MPN values of coliform bacteria in each drink collected. A total of 53.33% (16/30) of the drink samples showed positive E. coli detection. Then, the PCR assay showed 6.25% (one out of 16 isolates) samples were positive and carried stx1 gene produced by E. coli O157:H7 in iced milo sample types. This study showed the drinks collected from food premises was contaminated with faecal contamination, which was not safe to drink by the consumer. Therefore, preventive actions should be taken to prevent foodborne illness outbreak in future

scholarly journals Is Drinking Water Sources Safe for Drink? A Multicenter Assessment of Fecal Contamination of Drinking Water Sources in Tigray Region, Ethiopia

2019 ◽  
Author(s):  
Goyitom Gebremedhn ◽  
Abera Aregawi Berhe ◽  
Abraham Aregay Desta ◽  
Lemlem Legesse
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Fecal Coliform ◽  
Fecal Contamination ◽  
Water Sources ◽  
Capital City ◽  
Most Probable Number ◽  
Probable Number ◽  
Tigray Region ◽  
Drinking Water Sources

Abstract Background Fecal contamination of drinking water sources is the main cause of diarrhea with estimated incidence of 4.6 billion episodes and 2.2 million deaths every year. Methods A total of 145 water samples of different source type were collected from different areas in Tigray region from August 2018 to January 2019. The water samples from each site were selected purposively which involved sampling of water sources with the highest number of users and functionality status during the study period. Most Probable Number (MPN) protocol was used for the bacteriological analysis of the samples. Results A total of 145 water samples were collected from six zones in Tigray region, Ethiopia from August 2018 to January 2019. The study indicated that 63(43.5%) of the water samples were detected to have fecal coliform which is E.coli. In Mekelle city, which is the capital city of Tigray region, three in five 34(60.7%) of the collected samples were confirmed to have fecal coliform. Water samples from health facilities were 9.48 times [AOR=9.48, 95%CI: (1.59, 56.18)] more likely to have fecal coliform. Water samples from wells were 10.23 times [AOR=10.23, 95%CI: (2.74, 38.26)] more likely to have fecal coliform than water samples from Tap/Pipe. Similarly, water samples from hand pumps were 22.28 times [AOR=22.28, 95%CI: (1.26, 393.7)] more likely to have fecal coliform than water samples from Tap/Pipe. Water samples reported to be not chlorinated were 3.51 times [AOR=3.51, 95%CI: (1.35, 9.13)] more likely to have fecal coliform than water samples from chlorinated sources. Conclusion In this study all water source, including the chlorinated drinking water sources, were found highly contaminated with fecal origin bacteria. This may be mainly due to constructional defects, poor sanitation inspection, poor maintenance, intermittent water supply and irregular chlorination.

Microbiological Quality of Cocoa Powder, Dry Instant Chocolate Drink Mix, Dry Nondairy Coffee Creamer and Frozen Nondairy Topping Obtained at Retail Markets

1983 ◽  
Vol 46 (8) ◽  
pp. 733-736 ◽  
Author(s):  
W. L. PAYNE ◽  
A. P. DURAN ◽  
J. M. LANIER ◽  
A. H. SCHWAB ◽  
R. B. READ ◽  
...  
Keyword(s):  
Geometric Mean ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Cocoa Powder ◽  
Mean Values ◽  
Retail Markets ◽  
Coffee Creamer

A national survey was conducted of the microbiological quality of three dry ingredients used in beverages and one frozen non-dairy topping obtained at retail markets. Geometric mean aerobic plate counts (APCs) of units examined at 35°C were as follows: 1,313 units of cocoa powder, 6,600 CFU/g; 1,552 units of dry instant chocolate drink mix, 290 CFU/g; 1,559 units of dry non-dairy coffee creamer, 37 CFU/g; and 1,532 units of frozen non-dairy topping, 34 CFU/g. At 30°C, the geometric mean APC was 34 CFU/g for frozen nondairy topping. Geometric means for most probable number determinations of coliform bacteria and Escherichia coli were <3/g for the four products. Geometric mean values for Staphylococcus aureus in three of the products were <10/g; no S. aureus was found in cocoa powder. Geometric mean values for yeasts and molds in dry instant chocolate drink mix and dry nondairy coffee creamer were 8 and 6 CFU/g, respectively.

scholarly journals Drinking Water and Diarrheal Characteristic Cases in Darbendikhan City

2020 ◽  
Author(s):  
Sabah Shareef Mohammed ◽  
Yadgar Hussien Hama-karim ◽  
Mohammed Ibrahim Mohialdeen Gubari
Keyword(s):  
Drinking Water ◽  
Tap Water ◽  
Water Source ◽  
Water Sources ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Pipe System ◽  
Software Application ◽  
Drinking Water Sources

Water pollution in Iraq has been extended disaster edge; this pollution is caused by wastes and sewages into soil and rivers, pollutant water sources influence the outbreak and serious epidemic status among the population.. The aim of this study is to determine the bacteriological quality of drinking water sources and characteristics of water-borne diseases especially diarrheal cases in Darbandikhan city. 166 water samples, collected from the different sources and areas, were tested for the presences of coliform bacteria as an indicator for pathogen contamination. Most probable number index was used for coliform enumeration. 161 diarrheal cases were taken as a sample from the patients were admitted to the general hospital in Darbandikhan district.. The questionnaire form was planned to view characteristics of diarrheal cases and patients were interviewed directly, the data was analyzed by STATA software application. 46% of the diarrheal cases used tap water for drinking. On the other hand, the reminder (54%) used other sources for the same purpose. All the risk factor such as type of water source, sufficiency of the water, duration of water storage and chlorination were associated with diarrhea. Almost half of the cases were children and three quarters were single. The data was analyzed by STATA version 13.1.This study indicated that the majority of drinking water sources in Darbandikhan city are not suitable for drinking, although net pipe system supplied chlorine significantly it has been proven that the tap water from the homes is not suitable for consumption.

scholarly journals Water quality assessment based on microbiological parameter indicators for drinking water criteria on the Wadaslintang Reservoir

2020 ◽  
Vol 202 ◽  
pp. 05019
Author(s):  
Diana R. U. S. Rahayu ◽  
Sutrisno Anggoro ◽  
Tri R. Soeprobowati
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Government Regulation ◽  
Water Quality Management ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Microbiological Analysis ◽  
Raw Water ◽  
Reservoir Water ◽  
Probable Number

Wadaslintang Reservoir has potential as a source of raw water for drinking water. One indicator to determine water quality is to use coliform bacteria. The purpose of this study is to determine the water quality of the Wadaslintang Reservoir as a source of drinking water based on microbiological parameters by referring to Government Regulation of the Republic of Indonesia Number 82 of 2001 concerning water quality management and water pollution control. The water sampling method is based on purposive sampling at ten research stations for eight months, microbiological analysis using the Most Probable Number (MPN) method. The results showed that the status of the Wadaslintang Reservoir was polluted based on total coliform criteria. Utilization of reservoir water as raw water for drinking water needs to be done further processing to anticipate deterioration in health.

scholarly journals KUALITAS BAKTERIOLOGIS AIR MINUM ISI ULANGPADA TINGKAT PRODUSEN DI KABUPATEN BADUNG.

2015 ◽  
Vol 9 (2) ◽  
pp. 52
Author(s):  
Made Partiana ◽  
Made Sudiana Mahendra ◽  
Wayan Redi Aryanta
Keyword(s):  
Drinking Water ◽  
Water Supply ◽  
Significant Relationship ◽  
Raw Materials ◽  
Urban Community ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Bacteriological Examination ◽  
Probable Number

The presence of refill drinking water in the urban community especially Badung Regency is favored as an alternative of water supply because it cost relatively cheaper compared to water in pacage. The number of samples in this study was 45 outlets with refill drinking water as its study material. The bacteriological examination of the material of study was done on included most probable number (MPN) of coliform and Eschericia coli. The result of refill drinking water investigation bacteriological examination revealed 88.9 % outlets were considered qualified, 11.1 % of them were unqualified because they contained coliform bacteria and one stall had Eschericia coli. The observation hygiene 15.6 % unqualified and sanitation outlets examination 17.8 % unqualified. Correlation test showed no significant relationship between raw materials, equipment, processing, hygiene and sanitation autlets with bacteriological quality of drinking water

scholarly journals Implementation of Geographical Information System for Bacteriological Contamination Analysis on Refill Drinking Water Depot (Study in Tembalang District)

2018 ◽  
Vol 31 ◽  
pp. 06014
Author(s):  
Amelia Rahmitha ◽  
Endang Sri Utami ◽  
Marya Yenita Sitohang
Keyword(s):  
Information System ◽  
Drinking Water ◽  
Geographical Information System ◽  
Uv Light ◽  
Low Cost ◽  
Geographical Information ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Bacteriological Contamination

People used refilled-drinking-water for household and food stall because its efficient and low cost. Based on Indonesian Health Ministry regulation, it should not have any coliform bacteria. This study aimed to describe the bacteriological contamination of refilled drinking water using geographical information system (GIS). In this research, it was used an analytic observational method. The samples were from all available (37) depots in Tembalang district, one form each depot took used a sterile bottle. Contamination of bacteria was identified by Most Probable Number (MPN) method lactose broth media, Mac Conkey media, and IMVIC media. The depot samples were then plotted on (GIS). This study showed 95% samples were not feasible to consume since they contamined coliform. All sub-district had one that contaminated by coliform, 75% sub-districts had depots that contaminated Escherichia coli, while 55% sub-districts had depots that contaminated with other bacteria. The internal risk factors of the contamination were the absence of hygiene-sanitation worthy certificate (95%), depots location near to pollution sources (5%), and the misused of UV light. The external risk factor was lack of quality control that was not as the sterilization from office health Semarang city. Policy reinforcement should be done to all of the depots.

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