Fethiye Bölgesinde Açıkta Satılan Tüketime Hazır Bazı Gıdalarda Koliform Bakteri Sayısının Belirlenmesi

Since coliform bacteria are common both in the intestine and in nature (soil, plant, etc.), they are considered as a sanitation indicator in the food industry. It is known that the majority of bacteria defined as fecal coliform in the coliform group are Escherichia coli. The presence of E. coli or fecal coliform bacteria in any sample is an indication that the necessary hygienic measures are not taken during production, storage, and sale. That means the sample is directly or indirectly contaminated with faeces, and/or other intestinal pathogens may also exist. In this study, raw milk, freshly squeezed fruit juices, unpackaged ice cream, shaved ice, and ice-cold samples were purchased from the famous touristic destination Fethiye and analyzed for coliform bacteria. For this purpose, the samples were purchased from local marketplaces, buffets, cafes, patisseries, restaurants, and roadsides at Fethiye. In total 60 samples were analyzed using Violet Red Bile (VRB) Agar. The results of coliform bacteria ranged

Analysis of Sea Pollution by Sewage from Vessels

In this study, we analysed the sea pollution caused by sewage from vessels. The Dubrovnik aquatorium was chosen as a typical sea area that accommodates a variety of vessels in different locations. We sampled the sea at eight different coastal locations over 14 months and then analysed the samples to determine the presence of the indicators of fecal pollution. Simultaneous with the sampling of the sea, we recorded the number and type of vessels accommodated at the port. These data were applied in chi-square tests, which were used to determine the existence of the relationship of certain types of vessels with fecal coliform bacteria in the sea for each location. The correlation was determined between smaller vessels such as boats, yachts, megayachts, and smaller cruise ships in national navigation with bacteria at sea at the sampling locations. The results can provide an improved understanding of sea pollution due to sewage from vessels.

Evaluation of Dietary Curcumin Nanospheres in a Weaned Piglet Model

Curcumin is a polyphenolic compound present in turmeric with extensive uses in cooking foods and biomedical applications. However, due to its hydrophobic nature, it is poorly soluble in water and its bioavailability is very low on oral administration in organisms. In this study, we investigated the dietary curcumin nanospheres in a weaned piglet model based on the growth, serum biochemistry, proteomics, fecal coliform bacteria, and malodors in the feces of piglets. A total of 135 weaned piglets (Duroc × [Yorkshire × Landrace]) with an average initial body weight of 7.0 ± 1.0 kg (28 ± 1 days of age) were randomly distributed in 9 pens (15 pigs in each pen) fed the dietary curcumin nanospheres (CN) at 0 (control), 0.5 (T1), and 1.0 mL (T2) CN/kg of diet in triplicates for 21 days. At the end of the feeding trial, the results showed piglets fed 1.0 mL CN/kg diet had significantly higher growth performance and feed utilization than control diet (without CN). However, there were no significant differences in growth and feed utilization between piglets fed T1 and T2 diets. Serum glucose, gamma-glutamyl transferase, total bilirubin, amylase, and lipase contents were unaffected in piglets fed the experimental diets. Interestingly, piglets fed T1 and T2 diets showed significantly lower total cholesterol levels than control diet. In serum proteomics, a total of 103 differentially expressed proteins (DEPs) were identified in the piglets fed control, T1, and T2 diets, of which 14 DEPs were upregulated and 4 DEPs were downregulated. Fecal coliform bacteria and ammonia gas were significantly reduced in piglets fed T1 and T2 diets. Overall, the results indicated dietary supplementation of CN could enhance the growth, feed utilization, and immunity—and reduce fecal pathogenic bacteria as well as ammonia gas emissions—in weaned piglets.

Evaluation of the Effectiveness of the Activated Sludge Process in the Elimination Both ATB-Resistant and ATB-Susceptible E. coli Strains

Water reuse is now becoming a global necessity. However, one of the drawbacks in releasing wastewater into the environment is some persistent pollutants that are not completely removed in wastewater treatment plant. Residual bacteria and antibiotics in the inflowing wastewater can contribute to the antibiotic resistance spread in the aquatic environment. This study determined the effectiveness of activated sludge process for fecal coliform bacteria elimination, and also the Escherichia coli resistance to antimicrobial agents as erythromycin, azithromycin, clarithromycin, ofloxacin, ciprofloxacin, trimethoprim, and metronidazole in treated wastewater. The research was carried out using the membrane filtration technique, and the susceptibility of isolates to antimicrobial agents was tested by the disc diffusion method. The concentrations of fecal coliform bacteria and Escherichia coli differed significantly depending on the seasonal period in which it was carried out. Despite up to 99% reduction in the number of sanitary indicators in biologically treated wastewater, 89% of E. coli isolates resistant to the tested antibiotics was found, while 100% of the isolates were susceptible to metronidazole. Most of the isolates showed resistance to trimethoprim, and the fewest isolates were resistant to ofloxacin, indicating that some strains may react differently to antibiotics.

GROUNDWATER QUALITY IN BIRATNAGR OF MORANG DISTRICT, NEPAL

A study was conducted to assess the groundwater quality in Biratnagar of Morang district of Nepal on the basis of some important physicochemical and microbiological analysis. During the study, the elevated value of turbidity ranged from 2.1±2.80 NTU (ward no. 8) to 81.46±44.28 NTU (ward no. 10), free-carbondioxide (FCO2) ranged from 12±2.45 mg /L (ward no. 21) to 17.2±3.03 mg/L (ward no. 8), arsenic ranged from nil (ward no. 3) to 0.17±0.21 mg /L (ward no. 1), iron (Fe) ranged from 0.45 mg/L (ward no. 7) to 3.67 mg/L (ward no. 4), manganese (Mn), ranged from 0.45 mg/L (ward no. 7) to 5.99 mg/L (ward no. 16) and fecal coliform bacteria were found positive in 10 wards (ward nos. 2, 3, 4, 9, 10, 11,14,15,20 and 21) constituting 45.45% of the total samples ranging from 3 to 25 MPN/100 ml . Out of the total analyzed samples, turbidity in 78 samples (70.90%), FCO2, Fe, and Mn in all samples (100%) and arsenic in 37 water samples (33.64 %) were crossed the permissible limit of WHO guideline. However, the value of pH, EC, DO, TH, PO4-P, NO3-N, Cd, Zn, and Pb were below the WHO guideline value. The status of fluoride was below detectable level in all the analyzed groundwater samples.

Bacteria Identification In Traditional Sauce (Sambal) At The Restaurants Nearby The Traditional Markei Of Gorontalo City

The unsafe food will cause the health problems in the community. Sambal which is known as the traditional sauce is a sauce made from chilies that are crushed until the water content comes out, giving it a spicy taste. Furthermore, Sambal is processed in a simple way so that it can leac to the contamination of microorganisms such as Coliform bacteria contamination. This study aims to determine the presence of Coliform bacteria contamination in Sambal at the supermarket nearby the traditional market in Gorontalo City. This research is a descriptive study using a qualitative approach. It examines the existence of Coliform bacteria using the Most Probable Number (MPN) method. The population in this study were 14 samples with the sampling technique by accidental sampling. Coliform bacteria examination results show that Sambal sample is found with positive results contaminated with Coliform bacteria, namely 11 samples with a percentage of 78.6% and negative results of 3 samples with a percentage of 21.4%. Based on the results, it can be concluded that a positive result was found consisting of 1 sample of coliform fekal bacteria, in this case the bakteri Escherchia coli. bacteria, and 10 samples from the non-fecal coliform bacteria.

ANALISIS KUALITAS AIR PADA TIGA MATA AIR DI KECAMATAN SUKAWATI, KABUPATEN GIANYAR, BALI

Utilization of springs as water resources for their daily needs by the people of Sukawati Sub-Distric of Gianyar Regency, Bali is still quite high Human activities, such as increasing domestic, industrial and agricultural activities, have an effect on decreasing water quality. This research was conducted ini December 2019 to January 2020 at Pancoran Kutri springs, Beji Dalem Agung Kemenuh springs and Taman Beji Cengcengan springs. This research aimed to determine water quality based on Bali Governor's Regulation No.16 of 2016 concerning Environmental Quality Standards and Standard Criteria for Enviromental Damage Based on Class (Class I) and Minister of Health Republic of Indonesia Regulation No.492/ Menkes/Per/IV/2010 concerning Prerequisites for Drinking Water Quality. The parameters analyzed were the physical parameters (temperature and TDS), the chemical parameters (pH, DO, BOD, nitrate, phosphate, cadmium (Cd) and lead (Pb)) and the biological parameters (fecal coliform bacteria). The results of the research conducted showed that the water quality in the three springs in the study location still met the water quality standards. However, DO parameters at Pancoran Kutri springs, BOD parameters at Taman Beji Cengcengan springs and phosphate in the three springs at the research location are based on regulations, as well as fecal coliform bacteria parameters in the three springs at the research location based on the Regulation of the Minister of Health of the Republic of Indonesia No. 492/Menkes/Per/IV/2010 have exceeded the quality standard threshold.

Analysis of Coliform and Colifecal Total Pollution Test on Various Types of Drinking Water Using the MPN (Most Probable Number) Method

Water is the main source for life and also the most severe substance caused by pollution. The mandatory parameters for determining microbiological quality of drinking water are total non-fecal Coliform bacteria and Coliform fecal (Escherichia coli). Coliform bacteria are a group of microorganisms commonly used as indicators, where these bacteria can be a signal to determine whether a water source has been contaminated by bacteria or not, while fecal Coliform bacteria are indicator bacteria polluting pathogenic bacteria originating from human feces and warm-blooded animals (mammals) . The water inspection method in this study uses the MPN (Most Probable Number) method which consists of 3 tests, namely, the presumption test, the affirmation test, and the reinforcement test. The results showed that of 15 drinking water samples 8 samples were tested positive for Coliform bacteria with the highest total bacterial value of sample number 1, 15 (210/100 ml), while 7 other samples were negative. From 8 positive Coliform samples only 1 sample was stated to be negative fecal Coliform bacteria and 7 other samples were positive for Coliform fecal bacteria with the highest total bacterial value of sample number 1 (210/100 ml).