scholarly journals Comprehensive analysis of soluble RNAs in human embryo culture media and blastocoel fluid

2020 ◽  
Vol 37 (9) ◽  
pp. 2199-2209
Author(s):  
Kirstine Kirkegaard ◽  
Yan Yan ◽  
Boe S. Sørensen ◽  
Thorir Hardarson ◽  
Charles Hanson ◽  
...  
Keyword(s):  
Embryo Culture ◽  
Human Embryo ◽  
Culture Media ◽  
Comprehensive Analysis ◽  
Human Embryo Culture

scholarly journals A prospective randomized study comparing two commercially available types of human embryo culture media: G1-PLUSTM/G2-PLUSTM sequential medium (Vitrolife) and the GL BLASTTM sole medium (Ingamed)

2016 ◽  
Vol 20 (1) ◽  
Author(s):  
Ianae I. Ceschin ◽  
Mariana H. Ribas ◽  
Alvaro P. Ceschin ◽  
Lucileine Nishikawa ◽  
Claudia C. Rocha ◽  
...  
Keyword(s):  
Embryo Culture ◽  
Human Embryo ◽  
Culture Media ◽  
Randomized Study ◽  
Prospective Randomized Study ◽  
Human Embryo Culture

Determination of derivatized amino acids in human embryo culture media by gas chromatography

2007 ◽  
Vol 858 (1-2) ◽  
pp. 292-295 ◽  
Author(s):  
Mitja Križman ◽  
Irma Virant-Klun ◽  
Mirko Prošek
Keyword(s):  
Amino Acids ◽  
Gas Chromatography ◽  
Embryo Culture ◽  
Human Embryo ◽  
Culture Media ◽  
Human Embryo Culture

scholarly journals Noninvasive Metabolomic Profiling of Human Embryo Culture Media Using a Simple Spectroscopy Adjunct to Morphology for Embryo Assessment in in Vitro Fertilization (IVF)

2013 ◽  
Vol 14 (4) ◽  
pp. 6556-6570 ◽  
Author(s):  
Qinghong Zhao ◽  
Tailang Yin ◽  
Jin Peng ◽  
Yujie Zou ◽  
Jing Yang ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Culture ◽  
Human Embryo ◽  
Culture Media ◽  
Vitro Fertilization ◽  
Metabolomic Profiling ◽  
Human Embryo Culture

Soluble HLA-G (sHLA-G) in human embryo culture media: a useful marker for assessing embryo quality, and implantation potential for IVF

2003 ◽  
Vol 80 ◽  
pp. 12-13 ◽  
Author(s):  
Roumen Roussev ◽  
Levent Keskintepe ◽  
Carolyn Coulam ◽  
Geoffery Sher ◽  
Ghanima Maasarani
Keyword(s):  
Embryo Culture ◽  
Human Embryo ◽  
Embryo Quality ◽  
Culture Media ◽  
Soluble Hla ◽  
Human Embryo Culture

Water evaporation and osmolarity change of human embryo culture media in humid or in dry culture systems

2010 ◽  
Vol 94 (4) ◽  
pp. S151 ◽  
Author(s):  
C. Mori ◽  
M. Kuwayama ◽  
S.J. Silber ◽  
N. Kagawa ◽  
Y. Takehara ◽  
...  
Keyword(s):  
Embryo Culture ◽  
Human Embryo ◽  
Culture Media ◽  
Water Evaporation ◽  
Culture Systems ◽  
Human Embryo Culture

NGS Analysis of Human Embryo Culture Media Reveals miRNAs of Extra Embryonic Origin

2018 ◽  
Vol 26 (2) ◽  
pp. 214-222 ◽  
Author(s):  
Immaculada Sánchez-Ribas ◽  
Patricia Diaz-Gimeno ◽  
Alicia Quiñonero ◽  
María Ojeda ◽  
Zaloa Larreategui ◽  
...  
Keyword(s):  
Embryo Culture ◽  
Human Embryo ◽  
Culture Media ◽  
Embryonic Origin ◽  
Human Embryo Culture

P–769 FTIR spectroscopy analysis reveals differences between human embryo culture media composition by type of formulation and by manufacturer

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
I Ribeiro ◽  
F Pires ◽  
C R C Calado ◽  
M Gallard. Molina
Keyword(s):  
Fourier Transform ◽  
Ftir Spectroscopy ◽  
Embryo Culture ◽  
Human Embryo ◽  
Culture Media ◽  
Principal Component ◽  
Media Composition ◽  
Molecular Fingerprint ◽  
Media Type ◽  
Human Embryo Culture

Abstract Study question Can we detect the variation in different commercial human embryo culture media composition by Fourier-transform infrared (FTIR) spectroscopic analysis? Summary answer The spectra reveals distint features that allow distinguishing between continuous and sequential media, as well as between manufacturers of the same media type. What is known already: Manufacturers do not fully disclose commercially available culture media formulations. For this reason, it is important to gain insight into de differences between the available formulations, in order to understand how they might be linked with efficacy and safety ART parameters. Fourier Transform Infra-Red (FTIR) can be used for this purpose. Study design, size, duration Culture media samples (1 mL) were collected from local IVF laboratories, and stored frozen at –20 °C. Five repeats of 25 µL aliquots from the samples were analysed using FTIR spectroscopy to acquire the whole molecular fingerprint of each culture media. Participants/materials, setting, methods Three continuous (SAGE 1-STEP, Origio, G-TL, Vitrolife, and GERI, Genea) and four sequential (G1 PLUS, Vitrolife; Sequential Cleav with phenolred, Origio; G2 PLUS, Vitrolife and Sequential Blast with phenolred, Origio) media were analysed. Different pre-processing methods (atmospheric and baseline correction, normalizations, and derivatives) were carried out to minimize physical artefacts while highlighting chemical features. To compare the spectra of different media, multivariate analysis, as principal component analysis (PCA) and hierarchical cluster analysis (HCA) were employed. Main results and the role of chance The whole molecular fingerprint of all media analysed showed a similar pattern, revealing that, overall, the composition is very similar. However, PCA and HCA analysis revealed that significant differences exist, both between media type (continuous vs. sequential), and between different manufacturers within the same media type. Average linkage clustering using Spearman’s rank correlation confirms the similarities between the continuous and the sequential formulations. An analysis focusing the fingerprint region of the spectra (900 – 1700 cm^–1), also revealed variability between manufacturer, between media type (continuous vs. sequential) and of stage-specific media (cleavage vs. blastocyst). For instance, GERI media visually appeared to have distinct peaks compared to all other media, which was confirmed later through multivariate statistical analysis. Limitations, reasons for caution FTIR spectroscopy does not allow for a direct identification of the analytes present in the culture media, we can only infer the functional groups, but that are common on diverse biomolecules. Wider implications of the findings: FTIR analysis reveals differences between different media, such as cleavage and blastocyst specific, sequential and continuous, or manufacturer’s formulations. The increased resolution of the FTIR profile proves to be a powerful tool for analysing human embryo media, and could be used to establish correlations with media clinical performance and safety. Trial registration number Not applicable

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