Inhibition of LINE-1 retrotransposition represses telomere reprogramming during mouse 2-cell embryo development

2021 ◽  
Author(s):  
Fang Wang ◽  
Isaac J. Chamani ◽  
Danxia Luo ◽  
Kasey Chan ◽  
Paula Andrea Navarro ◽  
...  
Keyword(s):  
Embryo Development ◽  
Cell Embryo

scholarly journals Inhibition of DRP1 Impedes Zygotic Genome Activation and Preimplantation Development in Mice

2021 ◽  
Vol 9 ◽  
Author(s):  
Yuanyuan Li ◽  
Ning-Hua Mei ◽  
Gui-Ping Cheng ◽  
Jing Yang ◽  
Li-Quan Zhou
Keyword(s):  
Embryo Development ◽  
Preimplantation Embryo ◽  
Preimplantation Embryos ◽  
Dependent Manner ◽  
Zygotic Genome Activation ◽  
Preimplantation Embryo Development ◽  
Cell Embryo ◽  
Embryo Stage ◽  
Genome Activation ◽  
Zygotic Genome

Mitochondrion plays an indispensable role during preimplantation embryo development. Dynamic-related protein 1 (DRP1) is critical for mitochondrial fission and controls oocyte maturation. However, its role in preimplantation embryo development is still lacking. In this study, we demonstrate that inhibition of DRP1 activity by mitochondrial division inhibitor-1, a small molecule reported to specifically inhibit DRP1 activity, can cause severe developmental arrest of preimplantation embryos in a dose-dependent manner in mice. Meanwhile, DRP1 inhibition resulted in mitochondrial dysfunction including decreased mitochondrial activity, loss of mitochondrial membrane potential, reduced mitochondrial copy number and inadequate ATP by disrupting both expression and activity of DRP1 and mitochondrial complex assembly, leading to excessive ROS production, severe DNA damage and cell cycle arrest at 2-cell embryo stage. Furthermore, reduced transcriptional and translational activity and altered histone modifications in DRP1-inhibited embryos contributed to impeded zygotic genome activation, which prevented early embryos from efficient development beyond 2-cell embryo stage. These results show that DRP1 inhibition has potential cytotoxic effects on mammalian reproduction, and DRP1 inhibitor should be used with caution when it is applied to treat diseases. Additionally, this study improves our understanding of the crosstalk between mitochondrial metabolism and zygotic genome activation.

Micromolar concentration of pentoxifylline improves development in vitro of hamster 8-cell embryos: conrmation of biological viability by embryo transfer

1997 ◽  
Vol 9 (7) ◽  
pp. 697 ◽  
Author(s):  
Rupasri Ain ◽  
P. B. Seshagiri
Keyword(s):  
Embryo Transfer ◽  
Embryo Development ◽  
Human Sperm ◽  
Blastocyst Development ◽  
Preimplantation Embryo Development ◽  
Embryo Culture Medium ◽  
Cell Embryo ◽  
Continuous Presence ◽  
Development In Vitro

The inßuence of the sperm motility stimulant pentoxifylline (PF) on preimplantation embryo development in hamsters was evaluated. Eight-cell embryos were cultured in hamster embryo culture medium (HECM)-2, with or without PF (0· 0233·6 mM). There was 90%, 37% and 29% inhibition of blastocyst development by 3·6 (used for human sperm), 0·9 and 0 ·45 mM PF, respectively. However, 23 µM PF (exposed to hamster oocytes during IVF) signicantly (P < 0·05) improved blastocyst development (63· 6% v. 51· 8%); morulae development was, however, not curtailed by 0·45 mM or 0·9 mM PF (51·8%±6·0 or 50·5%±11·3, respectively). Post-implantation viability of PF-treated embryos was assessed by embryo transfer; 43% of 80 PF-treated embryos implanted compared with 40% of 79 control embryos. Of the 9 recipients, 6 females delivered pups (19, i.e. 16% of transferred embryos or 53% of implanted embryos). These data show that in hamsters, continuous presence of PF at 0·45-3·6 mM is detrimental to 8-cell embryo development whereas 23 µM PF improves the development of embryos to viable blastocysts which produce live offspring.

scholarly journals CRYOPRESERVATION OF SHEEP OOCYTES AND EMBRYOS USING LOCAL, MODIFIEID VITRIFICATION TOOL

2019 ◽  
Vol 50 (Special) ◽  
Author(s):  
Atiyah & et al.
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
In Vitro Maturation ◽  
Cost Effective ◽  
Normal Morphology ◽  
Vitro Fertilization ◽  
Immature Oocytes ◽  
Cell Embryo ◽  
First Time

The present study was aimed to cryopreserve mature, immature oocytes and in vitro produced embryos in Iraqi sheep using vitrification technique by local, simple and cost effective vitrification tool. This tool is an innovative straw called vitripeace invented, designed and used for the first time. Immature oocytes were aspirated from ovaries of slaughtered ewes and subjected to in vitro maturation and in vitro fertilization programs.The immature, mature oocytes and embryos were vitrified using Vitripeace tools, then thawed and assessed for the morphology and viability. The results revealed non-significant effect of time on viability (%) and normal morphology (%) of vitrified immature and mature oocytes for post-thawing and 2 hours post-thawing. The results showed significant (P<0.05) reduction   in the viability (%) of 2 cell embryo namely 88.89% and 77.78 %  for post-thawing and two hours post-thawing respectively. The results revealed a significant (P<0.05) reduction on normal morphology of 1 cell embryo namely 88.24 % and 76.47 %  for post-thawing  and two hours post-thawing respectively. Significant (P<0.05) differences in the percentage of normal morphology were found at post-thawing period for all stages of embryo development which were 90.74%, 88.31, 88.24 and 83.33 for immature , mature oocytes, 1 cell and 2 cell embryos, respectively while no significant differences in the viability at post-thawing period among all stages of embryo development. It was concluded that, successful vitrification of oocytes and embryos was resulted using Vitripeace which was novel, simple and cost effective vitrification tool.

scholarly journals Tracking intracellular forces and mechanical property changes in mouse one-cell embryo development

2020 ◽  
Vol 19 (10) ◽  
pp. 1114-1123 ◽  
Author(s):  
Marta Duch ◽  
Núria Torras ◽  
Maki Asami ◽  
Toru Suzuki ◽  
María Isabel Arjona ◽  
...  
Keyword(s):  
Mechanical Property ◽  
Embryo Development ◽  
Cell Embryo ◽  
Property Changes ◽  
Intracellular Forces

Effect of Short-Term Hypergravity Treatment on Mouse 2-Cell Embryo Development

2015 ◽  
Vol 27 (6) ◽  
pp. 465-471 ◽  
Author(s):  
Li-Na Ning ◽  
Xiao-Hua Lei ◽  
Yu-Jing Cao ◽  
Yun-Fang Zhang ◽  
Zhong-Hong Cao ◽  
...  
Keyword(s):  
Embryo Development ◽  
Short Term ◽  
Cell Embryo

Neem oil inhibits two-cell embryo development and trophectoderm attachment and proliferation in vitro

1994 ◽  
Vol 11 (8) ◽  
pp. 419-427 ◽  
Author(s):  
Subhash C. Juneja ◽  
Thea Pfeifer ◽  
R. Stan Williams ◽  
Nasser Chegini
Keyword(s):  
Embryo Development ◽  
Neem Oil ◽  
Cell Embryo ◽  
Proliferation In Vitro

scholarly journals Suplementasi Hormon Gonadotropin Pada Medium Maturasi In Vitro Untuk Meningkatkan Perkembangan Embrio Stadium 4 Sel Kambing Bligon

2016 ◽  
Vol 40 (2) ◽  
pp. 83 ◽  
Author(s):  
Dewi Pranatasari ◽  
Kustono Kustono ◽  
Diah Tri Widayati
Keyword(s):  
Embryo Development ◽  
In Vitro Maturation ◽  
Spherical Shape ◽  
Maturation Stage ◽  
Embryo Morphology ◽  
Cleavage Rate ◽  
Vitro Fertilization ◽  
Maturation Medium ◽  
Cell Embryo

The study was carried out to investigate the effect of gonadotropin hormone supplementation into in vitro maturation medium on maturation, fertilization and embryo development of Bligon goats. This research steps consist of oocyte collection, in vitro maturation, in vitro fertilization, and in vitro embryo development. At the maturation stage the oocyte that had been collected and divided into two groups based on the maturation medium, that was tissue culture medium (TCM) with supplementation of GnRH 0 IU/mL and GnRH 25 IU/mL. Oocyte and embryo morphology data were analyzed descriptively. Maturation rate and embryo development data were analyzed by using independent sample t-test. Fertilization data was analyzed descriptively. The result showed the percentages of mature oocytes from gonadotropin supplementation of 0 IU/mL and 25 IU/mL were 54.10±25.97 and 54.89±26.44%, respectively. Expansion cumulus cells surrounding the oocytes might indicated the mature oocytes. Cleavage rate of the 2 cells stage were 13,02±11,09 and 27,01±16,65%; respectively, and for the 4 cells stage were 10,16±10,01% and 16,67±14.91%. Embryos obtained from the treatment, indicated uniform of blastomeres in the size, tight, compact, intact, and round-spherical shape. It could be concluded that supplementation of gonadotropin hormone into in vitro maturation medium could not increase the rate of oocyte maturation and 4 cell embryo development, but it could increase 2 cell embryo development of Bligon goats. Hormone supplementation could improved the maturation and embryo quality.

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