Wound Healing Activity of Phage-Sisplayed TGF-β1 Model Peptide in Streptozotocin-Induced Diabetic Rats

Diabetic ulcers (DUs) are a chronic, non-healing diabetes complication that leads to high hospital expenses and, in extreme cases, to amputation. Peripheral vascular diseases, diabetic neuropathy, abnormal cellular and cytokine activity are among the major factors that hinder diabetic wound healing. DUs represent an important challenge in the development of new and efficient wound dressings. The extracellular matrix (ECM) has been effectively used as a scaffold for constructive remodeling of multiple tissues in animal and human. Sheep’s urinary bladder matrix was evaluated for its wound healing activity in streptozotocin-induced diabetic rats using excision model. In this experiment, 48 male Sprague dawley rats weighing 220–250[Formula: see text]g were divided into four equal groups of control, vaseline, diabetics + (10[Formula: see text]mg/wound) and [Formula: see text] (50[Formula: see text]mg/wound). Diabetes was induced by intraperitoneal injection of streptozotocin (45[Formula: see text]mg/kg B.W) solved in 0.05[Formula: see text]M citrate buffer. Seven days after confirming diabetes statue, skin wounds were created on the back of each rat. Rate of wound healing and histological assay using hematoxylin and Eosin staining (H&E) were used for evaluation of the wound healing in different groups. ECM treated animals exhibited significant improvement in both wound area and rate of wound healing when compared to controls ([Formula: see text]). The ECM treated wounds were found to epithelize faster as compared to controls. The sheep’s ECM promotes significant wound healing in male diabetic rats and further studies on this activity in animal models and humans are suggested.

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Wound healing activity of Terminalia bellerica Roxb. and gallic acid in experimentally induced diabetic animals

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2019-0133 ◽

2019 ◽

Vol 17 (2) ◽

Cited By ~ 1

Author(s):

Manish Pal Singh ◽

Avneet Gupta ◽

Siddhraj Singh Sisodia

Keyword(s):

Wound Healing ◽

Gallic Acid ◽

Dead Space ◽

Diabetic Rats ◽

Fruit Extract ◽

Standard Drug ◽

Terminalia Bellerica ◽

Wound Model ◽

Wound Healing Activity ◽

Experimentally Induced

AbstractBackgroundDiabetic wound are the major problem of society because of its delay healing of wound in hyperglycemia patient. The target of this study need to know the gallic acid compelling synergistically wound healing in diabetic animals as it is available in fruit extract or is more effective in given pure form in diabetic animals.MethodsThe wound healing effects of fruit extract Terminalia bellerica Roxb. and gallic acid were evaluated in experimentally induced hyperglycemic animals. In this investigation, excision and dead space wound models were used. The contraction wound %, re-epithelialization days, area of scar, histopathological examination of tissue, hydroxyproline level and weight of tissue granuloma were evaluated in this study.ResultsTerminalia bellerica fruit extract (400 mg/Kg) and gallic acid (200 mg/Kg) have been ingested by orally in streptozotocin induced diabetic rats. They were founded significantly (p<0.05) improved percentage wound contraction and decreased the size of scar area and days of re-epithelialization in excision wound model. Also, they were improved the level of hydroxyproline level in diabetic rats as compared to diabetic control group in dead space wound model. Standard drug vitamin C was also founded significantly improved wound healing activity in rats. These results suggested that treated groups accelerated wound healing activity in diabetic rat.ConclusionsThe observations and results obtained in the present study indicated the wound healing efficacy of fruit ethanolic extract and gallic acid in diabetic animals. Yet, the wound healing effect was increasingly reported with gallic acid.

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Wound healing activity ofWithania coagulansin streptozotocin-induced diabetic rats

Pharmaceutical Biology ◽

10.3109/13880209.2010.486837 ◽

2010 ◽

Vol 48 (12) ◽

pp. 1397-1404 ◽

Cited By ~ 29

Author(s):

S.K. Prasad ◽

R. Kumar ◽

D.K. Patel ◽

S. Hemalatha

Keyword(s):

Wound Healing ◽

Diabetic Rats ◽

Wound Healing Activity

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Wound healing activity of Pimpinella anisum methanolic extract in streptozotocin-induced diabetic rats

Journal of Wound Care ◽

10.12968/jowc.2019.28.sup10.s26 ◽

2019 ◽

Vol 28 (Sup10) ◽

pp. S26-S36

Author(s):

Mohammad Hashemnia ◽

Zahra Nikousefat ◽

Adel Mohammadalipour ◽

Mohammad-Mahdi Zangeneh ◽

Akram Zangeneh

Keyword(s):

Wound Healing ◽

Dry Matter ◽

Diabetic Rats ◽

Control Group ◽

Sprague Dawley ◽

Post Injury ◽

Pimpinella Anisum ◽

Total Antioxidant ◽

Healing Property ◽

Wound Healing Activity

Objective: To assess the wound healing potential of Pimpinella anisum on cutaneous wounds in diabetic rats. Method: Full-thickness excisional wounds were made on the back of male, Sprague-Dawley rats with diabetes. The rats were randomly allocated into four treatment groups: 1ml basal cream; tetracycline (3%); Pimpinella anisum 10% for 14 days; and a control group. At days seven, 14 and 21 post-injury, five animals of each group were euthanised, and wounds were assessed through gross, histopathological and oxidant/antioxidant evaluations. Additionally, the dry matter and hydroxyproline contents of the skin samples were measured. Results: A total of 60 rats were used in the study. A significant decrease in the wound size was observed in treated animals with Pimpinella anisum compared with other groups during the experiment. Additionally, treatment with Pimpinella anisum decreased the number of lymphocytes and improved the number of fibroblasts at the earlier stages and increased a number of fibrocytes at the later stages of wound healing. Other parameters such as re-epithelialisation, tissue alignment, greater maturity of collagen fibres and large capillary-sized blood vessels revealed significant changes when compared with the control. Pimpinella anisum significantly reverted oxidative changes of total antioxidant capacity, malondialdehyde and glutathione peroxidase induced by diabetic wounds (p<0.05). Furthermore, it significantly increased the dry matter and hydroxyproline contents at various stages of wound healing (p<0.05). Conclusion: The present study showed that application of Pimpinella anisum extract promotes wound healing activity in diabetic rats. The wound-healing property of Pimpinella anisum can be attributed to the phytoconstituents present in the plant.

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