Wound healing activity of Terminalia bellerica Roxb. and gallic acid in experimentally induced diabetic animals

2019 ◽  
Vol 17 (2) ◽  
Author(s):  
Manish Pal Singh ◽  
Avneet Gupta ◽  
Siddhraj Singh Sisodia
Keyword(s):  
Wound Healing ◽  
Gallic Acid ◽  
Dead Space ◽  
Diabetic Rats ◽  
Fruit Extract ◽  
Standard Drug ◽  
Terminalia Bellerica ◽  
Wound Model ◽  
Wound Healing Activity ◽  
Experimentally Induced

AbstractBackgroundDiabetic wound are the major problem of society because of its delay healing of wound in hyperglycemia patient. The target of this study need to know the gallic acid compelling synergistically wound healing in diabetic animals as it is available in fruit extract or is more effective in given pure form in diabetic animals.MethodsThe wound healing effects of fruit extract Terminalia bellerica Roxb. and gallic acid were evaluated in experimentally induced hyperglycemic animals. In this investigation, excision and dead space wound models were used. The contraction wound %, re-epithelialization days, area of scar, histopathological examination of tissue, hydroxyproline level and weight of tissue granuloma were evaluated in this study.ResultsTerminalia bellerica fruit extract (400 mg/Kg) and gallic acid (200 mg/Kg) have been ingested by orally in streptozotocin induced diabetic rats. They were founded significantly (p<0.05) improved percentage wound contraction and decreased the size of scar area and days of re-epithelialization in excision wound model. Also, they were improved the level of hydroxyproline level in diabetic rats as compared to diabetic control group in dead space wound model. Standard drug vitamin C was also founded significantly improved wound healing activity in rats. These results suggested that treated groups accelerated wound healing activity in diabetic rat.ConclusionsThe observations and results obtained in the present study indicated the wound healing efficacy of fruit ethanolic extract and gallic acid in diabetic animals. Yet, the wound healing effect was increasingly reported with gallic acid.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

2014 ◽  
Vol 1 (1) ◽  
pp. 73-77
Author(s):  
Uma Maheswari P ◽  
Shalimol A ◽  
Arumugasamy A ◽  
Udhaya Sankar M.R
Keyword(s):  
Wound Healing ◽  
Dry Weight ◽  
Wound Contraction ◽  
Albino Rats ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Wistar Albino Rats ◽  
Incision Wound ◽  
Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

WOUND HEALING ACTIVITY OF TRIPHALA IN EXPERIMENTALLY INDUCED DIABETIC RATS

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (04) ◽  
pp. 70-75
Author(s):  
M. P. Singh ◽  
S Vashisht ◽  
V. Chawla ◽  
P. Kumari ◽  
Keyword(s):  
Wound Healing ◽  
Oral Administration ◽  
Dead Space ◽  
Diabetic Rats ◽  
Wound Contraction ◽  
Control Group ◽  
Excision Wound ◽  
Wound Model ◽  
Diabetic Wound Healing ◽  
Healing Property

Aim of the present study was to explore the wound healing potential of Triphala extract. Triphala ointment (15% w/w) was used in the study for excision wound model. For dead space wound model Triphala extract (200 mg/kg) was administered orally. Extract of Triphala topically applied in excision wound model was found to possess wound healing property. On day 11th, the extract treated animals showed wound contraction to the extent of 87.63% and 94.77%, compared with control group of animals. In dead space wound model, oral administration of the extract was found to increase the wet and dry granuloma weight of tissue. The extract treated animals showed high quantity of hydroxyproline content when compared with the control group animals. The topical and oral administration of Triphala extract promotes diabetic wound healing in streptozotocin induced diabetic rats with increased rate of wound contraction and collagen turnover.

scholarly journals Studies on Wound Healing Activity of Heliotropium indicum Linn. Leaves on Rats

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
G. K. Dash ◽  
P. N. Murthy
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Dead Space ◽  
Methanol Extract ◽  
Infection Model ◽  
Aqueous Extracts ◽  
Reference Standard ◽  
Wound Model ◽  
Heliotropium Indicum ◽  
Wound Healing Activity

The petroleum ether, chloroform, methanol, and aqueous extracts of Heliotropium indicum Linn. (Family: Boraginaceae) were separately evaluated for their wound healing activity in rats using excision (normal and infected), incision, and dead space wound models. The effects of test samples on the rate of wound healing were assessed by the rate of wound closure, period of epithelialisation, wound breaking strength, weights of the granulation tissue, determination of hydroxyproline, super oxide dismutase (SOD), catalase, and histopathology of the granulation tissues. Nitrofurazone (0.2% w/w) in simple ointment I. P. was used as reference standard for the activity comparison. The results revealed significant promotion of wound healing with both methanol and aqueous extracts with more promising activity with the methanol extract compared to other extracts under study. In the wound infection model (with S. aureus and P. aeruginosa), the methanol extract showed significant healing activity similar to the reference standard nitrofurazone. Significant increase in the granulation tissue weight, increased hydroxyproline content, and increased activity of SOD and catalase level with the animals treated with methanol extract in dead space wound model further augmented the wound healing potential of H. indicum. The present work substantiates its validity of the folklore use.

The Extract of Lycium depressum Stocks Enhances Wound Healing in Streptozotocin-Induced Diabetic Rats

2017 ◽  
Vol 16 (2) ◽  
pp. 85-93 ◽  
Author(s):  
Siamak Naji ◽  
Leila Zarei ◽  
Masoumeh Pourjabali ◽  
Rahim Mohammadi
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Plant Material ◽  
Diabetic Rats ◽  
Male Rats ◽  
Impaired Wound Healing ◽  
Wound Model ◽  
Methanolic Extracts ◽  
Powder Extract ◽  
Wound Healing Activity

In diabetes, impaired wound healing and other tissue abnormalities are considered major concerns. The aim of the present study was to assess the wound-healing activity of methanolic extracts of the extract of Lycium depressum leaves. A total of 60 healthy male Wistar diabetic rats weighing approximately 160 to 180 g and 7 weeks of age were randomized into 10 groups for incision and excision wound models: sham surgery group (SHAM), including creation of wounds and no treatment; base formulation group (FG) with creation of wounds and application of base formulation ointment; treatment group 1 (TG1) with 1 g of powder extract of the plant material in ointment; treatment group 2 (TG2) with 2 g; and treatment group 4 (TG3) with 4 g of powder extract of the plant material in ointment. A wound was induced by an excision- and incision-based wound model in male rats. The mature green leaves of L depressum were collected and authenticated. Extractions of dried leaves were carried out. For wound-healing activity, the extracts were applied topically in the form of ointment and compared with control groups. The healing of the wound was assessed based on excision, incision, hydroxyproline estimation, and biomechanical and biochemical studies. The extract of L depressum leaves enhanced wound contraction, decreased epithelialization time, increased hydroxyproline content, and improved mechanical indices and histological characteristics in treatment groups compared with SHAM and FG ( P < .05). These findings permit the conclusion the extract of L depressum benefits parameters of wound healing in a diabetes induced model

scholarly journals Evaluation of Methanolic Extract of Hypericum Mysorense Ointment for its Wound Healing Activity

2020 ◽  
pp. 41-45
Author(s):  
Sankar C ◽  
Muthukumar S ◽  
Arulkumaran G ◽  
Vinesha R ◽  
Manimekalaim Manimekalaim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Methanolic Extract ◽  
Solid Dosage Forms ◽  
Standard Drug ◽  
Solid Dosage ◽  
Wound Model ◽  
Semi Solid ◽  
Wound Healing Activity ◽  
In Vitro Antioxidant Activity

The main objective of the present study is to formulate and evaluate semi-solid dosage forms of Hypericum mysorense for its wound healing activity. The stem of Hypericummysorense was extracted by continuous hot percolation/ soxhletationusing methanol as solvent and was evaluated for its phytochemical property, in-vitro antimicrobial and in-vitro antioxidant activity. Using this methanolic extract, semi-solid dosage form(ointment) was formulated. Ointment was prepared using 2% concentration of the extract by fusion method using emulsifying ointment.Hence, the ointment containing 2% methanolic extract of Hypericummy-sorensewas evaluated for its wound healing activity using excision wound model. The effect produced by the extract ointment, in terms of wound contracting ability, wound closure time and histopathological characteristics were comparable to that of a standard drug povidone iodine ointment. Therefore, the present study concluded that the Hypericum mysorense at 2% concentration of the methanolic extract formulated as an ointment showed a better wound healing formulation for better patient care and pharmaco economical.

scholarly journals GC-MS Analysis and Wound Healing Efficiency of Ethanolic Leaves Extract of Cassia occidentalis and Pithecellobium dulce in Wistar Albino Rats: Comparative Study

2021 ◽  
pp. 156-168
Author(s):  
B. Rajarajeswari ◽  
B. Praveen Kumar ◽  
Amrutha V. Audipudi
Keyword(s):  
Wound Healing ◽  
Dead Space ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Standard Group ◽  
Hydroxyproline Content ◽  
Cassia Occidentalis ◽  
Excision Wound ◽  
Wound Model ◽  
Wound Healing Activity

Aim: To identify and compare the bioactive compounds in the ethanolic leaves extracts of Cassia occidentalis and Pithecellobium dulce and to evaluate the wound healing efficiency in Wistar Albino rats. Study Design: The leaves ethanolic extracts was analysed by GC-MS and the extract was prepared in the form of a cream by ethanolic leaves extracts of C. occidentalis and P. dulce at 5% (w/v), 10% (w/v), and also in combination, a simple ointment base was developed with a composition of (1:1) Topical application of 5% (w/v) and 10% (w/v) was utilised in excision wound models. For excision wound models, the treatment duration was ten days. The day on which the wound was inflicted was designated as day '0'. Wound healing Activity: Excision wound Model: The animals were randomly separated into eight groups of six rats each: Group I: Control.; Group II: Standard group, treated with Framycetin sulfate cream (Soframycin, Aventis);. Group III: Treated with ethanolic extract of C. occidentalis (ELCO) (5% w/v); Group IV: Treated with ethanolic extract of C. occidentalis (ELCO) (10% w/v);Group V: Treated with ethanolic extract of P. dulce (ELPD)(5% w/v); Group VI: Treated with ethanolic extract of P. dulce (ELPD) (10 % w/v);Group VII: Treated with ethanolic extract of C. occidentalis and P. dulce (ELCO & ELPD 1:1) (5% w/v); Group VIII: Treated with ethanolic extract of C. occidentalis and P. dulce (ELCO & ELPD 1:1) (10 % w/v) till complete epithelization. Next dead space wound model and histology was studied. Place and Duration of Study: The GC-MS was carried out at Lab in Chennai. The extraction procedures were done at Department of Microbiology, Acharya Nagarjuna University, Guntur and treatment of wound healing activities were conducted at Ratnam Institute of Pharmacy in Nellore, Andhra Pradesh, India, and housed in the Department of Pharmacology between October to January 2016. Methodology: To study bioactive compounds, GC-MS was adopted, for wound healing activity: Excision wound Model, Dead space wound model and histology procedures was applied. Results:  In the current study, ethanol leaves extract (EL) of Cassia occidentalis and Pithecellobium dulce were compared using GC-MS and their wound healing efficacy in wistar rats was examined. The GC-MS analysis of EL from both plants revealed 14-16 distinct bioactive phytochemical components with varying molecular weights and retention duration (RT). Excision and dead space wound models were utilised to assess the wound healing activities of EL extracts on rats. Wound concentration, full epithelialzation time, granulation, tissue weight, and hydroxyproline content were used to measure healing. In the excision wound model, the standard group (Framycetin sulphate cream) and group-VII (10% w/v; 1:1) combination EL treatment exhibited 98.5 ± 0.54 % and 98.4 ± 0.46 % wound healing activity, respectively. When compared to the control, the granulation tissue weight and hydroxyproline content in the dead space wound rose considerably. Histological examination revealed fewer inflammatory cells and more collagen, indicating a role in accelerating wound healing activity. Conclusion: The results of our investigation indicate unequivocally that ethanolic leaf extracts of these plant species are effective at encouraging wound healing. The 10% (ELCO+ELPD) tropical treatment drastically reduced the wound as compared to standard and also increased granulation and hydroxyproline content. However, it requires more clinical examination before being considered for wound therapy.

scholarly journals Pharmacological evaluation of wound healing activity of herbal based formulation “Amree Plus®” in diabetic rats

2017 ◽  
Vol 6 (1) ◽  
pp. 1-10
Author(s):  
Sunita Thakur ◽  
◽  
Isha Dhamija ◽  
Sandeep Kumar ◽  
Anil Kumar Sharma ◽  
...  
Keyword(s):  
Wound Healing ◽  
Diabetic Rats ◽  
Proline Content ◽  
Wound Contraction ◽  
Total Protein Content ◽  
Delayed Wound Healing ◽  
Polyherbal Formulation ◽  
Wound Model ◽  
Herbal Formulation ◽  
Wound Healing Activity

Delayed wound healing is producing high economic burden on the society and is generally associated with Diabetes mellitus or related complications thus needs safe treatment like herbal formulation. „Amree Plus® (an anti-diabetic ayurvedic formulation)‟ was evaluated for wound healing potential in normal and diabetic rats. The effect of polyherbal formulation Amree plus ® on wound healing has been studied in diabetic and normal animals and compared with standard (Chymoral Forte) using excision wound model. Diabetes was induced by administration of single dose of Streptozotocin 55 mg/kg i.p. The biochemical parameters i.e. hydroxyl proline content, total protein content, glutathione and superoxide dismutase (SOD) were also estimated. Rats treated with Amree Plus® at both doses i.e. 1200 and 5400 mg/kg dose per day had significantly high wound contraction both in diabetic and normal rats (88.91 & 95.05 %, respectively) in comparison to respective control. Hydroxyproline content of the granulation tissue and anti-oxidants (glutathione and SOD) level were also showing same pattern in Amree Plus® 5400 mg/kg treatment group with respect to controls. Wound contraction and other biochemical indicators results with animals Amree Plus® (1200 mg/kg) treated were found to be statistically equivalent to standard chymoral forte dose (36 kAU/kg) while Amree Plus® 5400 mg/kg dose showed statistically higher results than standard. Amree Plus®, an established antidiabetic formulation, was also showing healing of the wound in normal animals along with diabetic animals. So this finding should be confirmed further for more precision with large number of animals and small progression of doses.

scholarly journals WOUND HEALING ACTIVITY OF JATROPHA TANJORENSIS LEAVES

2018 ◽  
Author(s):  
Madepalli Byrappa gowdu Viswanathan ◽  
Jesu Doss Jeya Ananthi ◽  
NataRajan Livingston Raja ◽  
Narayanan Venkateshan
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Methanol Extract ◽  
Chloroform Extract ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Model ◽  
And Function ◽  
Incision Wound ◽  
Wound Healing Activity

The term “wound” is defined as a disruption of normal anatomical structure. Therefore, “healing” is the complex and dynamic process that results in the restoration of anatomical continuity and function. Albino Wistar rats (150-180 g) of both sexes were selected. The experiment of Wound Healing Activity by Excision Wound Model and Incision Wound Model by the simple ointment B.P., reference standard drug (0.2% w/w nitrofurazone ointment), stigmasterol ointment (0.2% w/w), hexane, chloroform and methanol extract ointments of 3%, 4% and 5% w/w of leaves in Jatropha tanjorensis in simple ointment base (where 3g, 4g and 5 g of the extracts was incorporated in 100 g of simple ointment separately). The time for wound closure to methanol extract ointment (5% w/w) and stigmasterol (0.2% w/w) was similar to that of standard drug, nitrofurazone ointment (0.2% w/w) 16 ± 2 days in Excision Wound Model. The significant tensile strength at 3%: 4%: 5% w/w methanol extract ointments (p<0.001), followed by chloroform extract and hexane extract. Stigmasterol ointment at 0.2% w/w produced tensile strength comparable with Standard drug, nitrofurazone ointment (0.2% w/w) (p<0.001) in Incision Wound Model.  Kirithikar and basu stated that the juice of the Jatropha plant and the pounded leaves are applied to wounds and refractory ulcers. The juice is very successfully used to treat scabies, eczema and ringworm. The present study proved that the leaves have wound healing activity.

Comparison of the ficus for the wound healing activity in various species

2020 ◽  
Vol 10 (4) ◽  
pp. 67-70
Author(s):  
Mothilal K ◽  
Akila CR ◽  
Mahender K ◽  
Chaitanya Kumar K ◽  
Ravi D
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Subcutaneous Tissue ◽  
Significant Activity ◽  
Herbal Extracts ◽  
Standard Drug ◽  
Excision Wound ◽  
Injuries And Wounds ◽  
Skin Conditions ◽  
Wound Healing Activity

Injuries and wounds are any sorts of damage to the skin or subcutaneous tissue. Usually, any wounds of such sorts are self-healed. Sometimes, there may be a delay in healing, and that delay is caused due to the functional delays in various processes of wound healing. All the Ficus plants show similar activities like the antioxidant, anti-inflammatory and wound healing properties 7including skin conditions like ulcers and rheumatism. The anthelmintic property and immunomodulatory are also seen. The herbal extracts of the same family of Ficus in different plants were investigated for the wound healing activity in the excision wound method, and the extracts showed significant activity compared to the drug. All the extracts showed a better healing ability, but the extract of FBO-100 showed the highest activity followed by FMO followed by FHO and finally the FRO. Overall, the activity of the extract ointment was comparable and was significant compared to the standard drug ointment. The wound closure of the extract ointment treated groups were better and were completed in 12 days, and the activity was more than 96%. The herbal extracts of the same family of Ficus in different plants were investigated for the wound healing activity in the excision wound method, and the extracts showed significant activity compared to the drug. The plants of microcarpa, benghalensis, religiosa and hispida are compared for the activity, and the order showed for the activity was FBO>FMO>FHO>FRO.

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