Development of robust molecular markers for marker-assisted selection of leaf rust resistance gene Lr23 in common and durum wheat breeding programs

Wheat leaf rust, caused by Puccinia triticina, is a common fungal disease of wheat in China. In order to identify races and determine the individual virulence of isolates in different wheat-growing regions in China, leaf rust samples collected from 18 provinces in 2011 to 2013 were tested on 37 Thatcher near-isogenic lines each carrying a different single leaf rust resistance gene. A total of 158 races were identified. Races THTT (19.5%), THTS (16.9%), PHTT (7.7%), THJS (5.0%), THJT (4.2%), and PHTS (4.0%) were the most predominant races in 2011 to 2013. All of these races were avirulent to resistance genes Lr9 and Lr24. The two most frequent races, THTT and THTS, were widely distributed. The frequencies of the isolates with virulence to Lr1, Lr2c, Lr3, Lr16, Lr26, Lr17, LrB, Lr10, Lr14a, Lr3bg, Lr14b, Lr33, Lr37, and Lr50 exceeded 90%. Frequencies of virulence to Lr2a, Lr3ka, Lr11, Lr30, Lr2b, and Lr32 exceeded 70% but were less than 90%. Frequencies of virulence to Lr18, Lr21, Lr15, Lr23, Lr33+34, Lr36, Lr39, and Lr44 were below 70%, whereas the frequency of virulence to Lr25 was less than 1%. All isolates were avirulent to Lr9, Lr19, Lr24, Lr28, Lr42, Lr29, Lr38, and Lr47. The identified races and individual virulence frequencies provide a basis for selection of effective leaf rust resistance genes for use in breeding programs and can also provide information for the study of race evolution of P. triticina.

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Identification of stem rust and leaf rust resistance genes in Amagalon oats

Australian Journal of Agricultural Research ◽

10.1071/ar01084 ◽

2001 ◽

Vol 52 (10) ◽

pp. 1011 ◽

Cited By ~ 2

Author(s):

K. N. Adhikari ◽

R. A. McIntosh

Keyword(s):

Leaf Rust ◽

Stem Rust ◽

Rust Resistance ◽

Recessive Gene ◽

Leaf Rust Resistance ◽

Leaf Rust Resistance Gene ◽

Breeding Programs ◽

Sensitivity Tests ◽

Unique Source ◽

Leaf Rust Resistance Genes

Studies were undertaken to identify the genes conferring stem rust and leaf rust resistances in Amagalon and to determine the usefulness of this line as a source of rust resistance in oat breeding programs. Amagalon was crossed with certain rust-resistant and rust-susceptible lines and segregating populations were tested with pathotypes of Puccinia graminis avenae and P. coronata avenae. Tests with the widely virulent P. graminis avenae pt 94+Pg-13 indicated that resistance in Amagalon was governed by the complementary recessive gene complex known as Pg-a. This hypothesis was further substantiated by temperature sensitivity tests and by a test of induced susceptibility to stem rust, known to be unique to lines possessing Pg-a. However, Amagalon yielded a unique source of resistance to leaf rust that was effective against current pathotypes of P. coronata avenae in Australia. This gene, assumed to be Pc91, was inherited independently of a second leaf rust resistance gene present in cv. Culgoa. It was concluded that Amagalon is a useful source of resistance to leaf rust that should be used in combination with other genes for resistance to prolong its effectiveness.

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Identification and Molecular Characterization of Leaf Rust Resistance Gene Lr14a in Durum Wheat

Plant Disease ◽

10.1094/pdis-92-3-0469 ◽

2008 ◽

Vol 92 (3) ◽

pp. 469-473 ◽

Cited By ~ 43

Author(s):

S. A. Herrera-Foessel ◽

R. P. Singh ◽

J. Huerta-Espino ◽

H. M. William ◽

V. Garcia ◽

...

Keyword(s):

Durum Wheat ◽

Leaf Rust ◽

Resistance Gene ◽

Ssr Markers ◽

Common Wheat ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Leaf Rust Resistance Gene

Leaf rust, caused by Puccinia triticina, is an important disease of durum wheat (Triticum turgidum subsp. durum) and only a few designated resistance genes are known to occur in this crop. A dominant leaf rust resistance gene in the Chilean durum cv. Llareta INIA was mapped to chromosome arm 7BL through bulked segregant analysis using the amplified fragment length polymorphism (AFLP) technique, and by mapping three polymorphic markers in the common wheat (T. aestivum) International Triticeae Mapping Initiative population. Several simple sequence repeat (SSR) markers, including Xgwm344-7B and Xgwm146-7B, were associated with the leaf rust resistance gene. Resistance response and chromosomal position indicated that this gene is likely to be Lr14a. The SSR markers Xgwm344-7B and Xgwm146-7B and one AFLP marker also differentiated common wheat cv. Thatcher from the near-isogenic line with Lr14a, as well as durum ‘Altar C84’ from durum wheat with Lr14a. This is the first report of the presence of Lr14a in durum wheat, although the gene originally was transferred from emmer wheat ‘Yaroslav’ to common wheat. Lr14a is also present in CIMMYT-derived durum ‘Somateria’ and effective against Mexican and other P. triticina races of durum origin. Lr14a should be deployed in combination with other effective leaf rust resistance genes to prolong its effectiveness in durum wheat.

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Identification and Mapping of Lr3 and a Linked Leaf Rust Resistance Gene in Durum Wheat

Crop Science ◽

10.2135/cropsci2006.10.0663 ◽

2007 ◽

Vol 47 (4) ◽

pp. 1459-1466 ◽

Cited By ~ 29

Author(s):

Sybil A. Herrera-Foessel ◽

Ravi P. Singh ◽

Julio Huerta-Espino ◽

Manilal William ◽

Garry Rosewarne ◽

...

Keyword(s):

Durum Wheat ◽

Leaf Rust ◽

Resistance Gene ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Leaf Rust Resistance Gene

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Highly predictive SNP markers for efficient selection of the wheat leaf rust resistance gene Lr16

BMC Plant Biology ◽

10.1186/s12870-017-0993-7 ◽

2017 ◽

Vol 17 (1) ◽

Cited By ~ 20

Author(s):

Mulualem T. Kassa ◽

Frank M. You ◽

Colin W. Hiebert ◽

Curtis J. Pozniak ◽

Pierre R. Fobert ◽

...

Keyword(s):

Leaf Rust ◽

Resistance Gene ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Snp Markers ◽

Leaf Rust Resistance Gene ◽

Wheat Leaf Rust ◽

Wheat Leaf ◽

Efficient Selection ◽

Selection Of

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Validation and identification of molecular markers linked to the leaf rust resistance gene Lr28 in wheat

Journal of Applied Genetics ◽

10.1007/s13353-010-0026-9 ◽

2011 ◽

Vol 52 (2) ◽

pp. 171-175 ◽

Cited By ~ 15

Author(s):

Annapurnalilly Bipinraj ◽

Balgounda Honrao ◽

Mohinder Prashar ◽

Subhash Bhardwaj ◽

Suryaprakasa Rao ◽

...

Keyword(s):

Molecular Markers ◽

Leaf Rust ◽

Resistance Gene ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Leaf Rust Resistance Gene

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Fine Mapping of the Wheat Leaf Rust Resistance Gene Lr42

International Journal of Molecular Sciences ◽

10.3390/ijms20102445 ◽

2019 ◽

Vol 20 (10) ◽

pp. 2445 ◽

Cited By ~ 8

Author(s):

Harsimardeep S. Gill ◽

Chunxin Li ◽

Jagdeep S. Sidhu ◽

Wenxuan Liu ◽

Duane Wilson ◽

...

Keyword(s):

Leaf Rust ◽

Hexaploid Wheat ◽

Rust Resistance ◽

Reference Genome ◽

Aegilops Tauschii ◽

Leaf Rust Resistance ◽

Wheat Breeding ◽

Chromosome 1 ◽

Adult Plant ◽

Leaf Rust Resistance Gene

Leaf rust caused by Puccinia triticina Eriks is one of the most problematic diseases of wheat throughout the world. The gene Lr42 confers effective resistance against leaf rust at both seedling and adult plant stages. Previous studies had reported Lr42 to be both recessive and dominant in hexaploid wheat; however, in diploid Aegilops tauschii (TA2450), we found Lr42 to be dominant by studying segregation in two independent F2 and their F2:3 populations. We further fine-mapped Lr42 in hexaploid wheat using a KS93U50/Morocco F5 recombinant inbred line (RIL) population to a 3.7 cM genetic interval flanked by markers TC387992 and WMC432. The 3.7 cM Lr42 region physically corresponds to a 3.16 Mb genomic region on chromosome 1DS based on the Chinese Spring reference genome (RefSeq v.1.1) and a 3.5 Mb genomic interval on chromosome 1 in the Ae. tauschii reference genome. This region includes nine nucleotide-binding domain leucine-rich repeat (NLR) genes in wheat and seven in Ae. tauschii, respectively, and these are the likely candidates for Lr42. Furthermore, we developed two kompetitive allele-specific polymorphism (KASP) markers (SNP113325 and TC387992) flanking Lr42 to facilitate marker-assisted selection for rust resistance in wheat breeding programs.

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Incorporation of leaf rust resistance genes into winter wheat genotypes using marker-assisted selection

Acta Agronomica Hungarica ◽

10.1556/aagr.55.2007.2.2 ◽

2007 ◽

Vol 55 (2) ◽

pp. 149-156 ◽

Cited By ~ 5

Author(s):

M. Gál ◽

G. Vida ◽

A. Uhrin ◽

Z. Bedő ◽

O. Veisz

Keyword(s):

Winter Wheat ◽

Leaf Rust ◽

Resistance Genes ◽

Marker Assisted Selection ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Wheat Breeding ◽

Wheat Varieties ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

The breeding and cultivation of resistant wheat varieties is an effective way of controlling leaf rust ( Puccinia triticina Eriks.). The use of molecular markers facilitates the incorporation of the major leaf rust resistance genes ( Lr genes) responsible for resistance into new varieties and the pyramiding of these genes. Marker-assisted selection was used to incorporate the Lr genes currently effective in Hungary ( Lr9 , Lr24 , Lr25 , Lr29 ) into winter wheat varieties. The Lr genes were identified using STS, SCAR and RAPD markers closely linked to them. Investigations were made on how these markers could be utilised in plant breeding, and near-isogenic lines resembling the recurrent variety but each containing a different Lr gene were developed to form the initial stock for the pyramiding of resistance genes. The results indicate that the marker-assisted selection technique elaborated for resistance genes Lr24 , Lr25 and Lr29 can be applied simply and effectively in wheat breeding, while the detection of the Lr9 marker is uncertain.

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Molecular Mapping of a Leaf Rust Resistance Gene on the Short Arm of Chromosome 6B of Durum Wheat

Plant Disease ◽

10.1094/pdis-92-12-1650 ◽

2008 ◽

Vol 92 (12) ◽

pp. 1650-1654 ◽

Cited By ~ 20

Author(s):

S. A. Herrera-Foessel ◽

R. P. Singh ◽

J. Huerta-Espino ◽

H. M. William ◽

A. Djurle ◽

...

Keyword(s):

Durum Wheat ◽

Leaf Rust ◽

Resistance Gene ◽

Common Wheat ◽

Rust Resistance ◽

Molecular Mapping ◽

Infection Type ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Leaf Rust Resistance Gene

Leaf rust, caused by Puccinia triticina, is an important disease of durum wheat (Triticum turgidum subsp. durum) worldwide, and the most effective way to control it is through the use of resistant cultivars. A partially dominant leaf rust resistance gene present in the International Maize and Wheat Improvement Center-derived Chilean cv. Guayacan INIA and its sister line Guayacan 2 was mapped to chromosome arm 6BS by identifying linked amplified fragment length polymorphisms (AFLPs) and mapping two of the molecular markers in common wheat (T. aestivum) linkage maps of the International Triticeae Mapping Initiative and Oligoculm × Fukuho-komugi populations. Comparison of infection type responses of the two resistant durums with common wheat testers carrying the previously mapped resistance genes Lr36 and Lr53 on 6BS, and their chromosomal positions, indicated that the resistance gene in durum wheat Guayacan INIA is a new leaf rust resistance gene, which was designated as Lr61. Gene Lr61 is effective against the P. triticina race BBG/BN predominant in northwestern Mexico and other races infecting durum wheat in various countries.

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