Isolation and characterization of a virulent bacteriophage SPW specific for Staphylococcus aureus isolated from bovine mastitis of lactating dairy cattle

2014 ◽  
Vol 41 (9) ◽  
pp. 5829-5838 ◽  
Author(s):  
Longping Li ◽  
Zhiying Zhang
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cattle ◽  
Bovine Mastitis ◽  
Isolation And Characterization ◽  
Lactating Dairy Cattle

Isolation and characterization of two new Staphylococcus aureus bacteriophages with potential to infect distinct bacteria from bovine mastitis

2021 ◽  
Author(s):  
Bibiana Martins Barasuol ◽  
Juliana Felipetto Cargnelutti ◽  
Luis Antônio Sangioni ◽  
Daniela Isabel Brayer Pereira ◽  
Ana Paula Muterle Varela ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bovine Milk ◽  
Bovine Mastitis ◽  
Gc Content ◽  
Etiologic Agent ◽  
Cure Rate ◽  
Long Tail ◽  
Isolation And Characterization ◽  
Double Stranded Dna

Abstract Bovine mastitis is an important disease of dairy cows, and Staphylococcus aureus is the etiologic agent most prevalent among the microorganisms. Mastitis caused by S. aureus present low cure rate with antimicrobials treatment and low vaccines efficacy. Bacteriophages or phages have been considered as an alternative for treating this disease. This study, we isolated and characterized two new S. aureus phages, namely B_UFSM4 and B_UFSM5, from bovine milk of cows with mastitis. The adsorptions rates were 10–20 min for B_UFSM4 and 20–30 min for B_UFSM5. Phages activities were relatively stable at pH 3–11; however, at temperatures of 50 °C-60ºC-70ºC/60 min, the phages were completely inactivated. These viruses presented infectivity in various bacteria isolated from bovine mastitis, where the lytic activity of phages B_UFSM4 and B_UFSM5 were 34.2%(13/38) and 42.1%(16/38), respectively, including isolates from S. aureus, Pseudomonas aeruginosa, Staphylococcus sciuri, and Rothia terrae. The complete genomes of B_UFSM4 and B_UFSM5 have 41.396 bp and 41.829 bp, with GC-content 33.97% and 33.98%, respectively. Both phages comprise 61 putative ORFs. The viruses have double stranded DNA and linear architecture. Phylogenic similarity was observed by proteome with Staphylococcus prophage phiPV83 (45,536 nt), Staphylococcus phage CN125 (44,492 nt) and Staphylococcus phage JS01 (43,458 nt). Based on the morphology, the phages belong to Siphoviridae family, presenting icosahedral head with a long tail, Caudovirales order and Biseptimavirus genus. Thus, two S. aureus phages (B_UFSM4 and B_UFSM5) were isolated and characterized, and these phages can be used as therapeutic or prophylactic candidates against S. aureus infections in cattle mastitis.

scholarly journals Isolation and characterization of Staphylococcus aureus from bovine mastitis in Andhra Pradesh

2021 ◽  
Vol 74 (5) ◽  
pp. 428-433
Author(s):  
P Madhava ◽  
D Rani Prameela ◽  
B Sreedevi ◽  
T Madhava Rao
Keyword(s):  
Staphylococcus Aureus ◽  
Andhra Pradesh ◽  
Bovine Mastitis ◽  
Isolation And Characterization

scholarly journals Proteomic Characterization of Lytic Bacteriophages of Staphylococcus aureus Isolated from Sewage Affluent of India

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Kamalpreet Kaur Sangha ◽  
B. V. Sunil Kumar ◽  
Ravi Kant Agrawal ◽  
Dipak Deka ◽  
Ramneek Verma
Keyword(s):  
Staphylococcus Aureus ◽  
Bovine Mastitis ◽  
Soft Agar ◽  
Culture Collection ◽  
Economic Consequences ◽  
Icosahedral Symmetry ◽  
Bacteriophage Therapy ◽  
Tryptone Soya Agar ◽  
Isolation And Characterization

Staphylococcus aureus is a Gram-positive bacterium that causes a variety of diseases, including bovine mastitis, which has severe economic consequences. Standard antibiotic treatment results in selection of resistant strains, leading to need for an alternative treatment such as bacteriophage therapy. Present study describes isolation and characterization of a staphylococcal phage from sewage samples. S. aureus isolates obtained from microbial type culture collection (MTCC), Chandigarh, India, were used to screen staphylococcal phages. A phage designated as ΦMSP was isolated from sewage samples by soft agar overlay method. It produced clear plaques on tryptone soya agar overlaid with S. aureus. Transmission electron microscopy revealed that the phage had an icosahedral symmetry. It had 5 major proteins and possessed a peptidoglycan hydrolase corresponding to 70 kDa. ΦMSP infection induced 26 proteins to be uniquely expressed in S. aureus. This phage can be proposed as a candidate phage to treat staphylococcal infections.

scholarly journals Detection of β-lactamase, blaZ and mecA in penicillin-resistant Staphylococcus aureus isolated from bovine mastitis in Garanhuns, Brazil

2021 ◽  
Vol 15 (2) ◽  
pp. 140-145
Author(s):  
Amanda Pereira Lucas ◽  
Andriele Renata Barbosa de Farias ◽  
Elizabete Cristina da Silva ◽  
Kleber Régis Santoro ◽  
Marcelo Mendonça ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cattle ◽  
Meca Gene ◽  
Resistance Mechanism ◽  
Bovine Mastitis ◽  
Duplex Pcr ◽  
Genetic Determinants ◽  
The City

There are few reports in the literature about genetic determinants of resistance to β-lactams in Staphylococcus aureusisolated from dairy cattle located in the municipality of Garanhuns, state of Pernambuco, Brazil. Thus, this study aimed to investigate the production of β-lactamase and the presence of the blaZ and mecA genes in penicillin-resistant S. aureus isolated from cases of subclinical bovine mastitis in the city of Garanhuns. Forty-six strains of penicillin-resistant S. aureus were evalu-ated using the nitrocefin disc test and duplex PCR. The results revealed that 45 strains (97.8%) were positive for β-lactamase production and 44 (95.7%) carried the blaZ gene. Among the latter, 43 (97.7%) were β-lactamase producers and only one (2.3%) was not. The mecA gene was not detected in any of the isolates investigated. The results suggest that enzymatic inacti-vation is the main β-lactam resistance mechanism expressed by S. aureus in the herds analyzed.

Isolation and characterization of three benzylisoquinoline alkaloids from Thalictrum minus L. and their antibacterial activity against bovine mastitis

2016 ◽  
Vol 193 ◽  
pp. 221-226 ◽  
Author(s):  
Saleem Mushtaq ◽  
Muzafar Ahmad Rather ◽  
Parvaiz H. Qazi ◽  
Mushtaq A. Aga ◽  
Aabid Manzoor Shah ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bovine Mastitis ◽  
Benzylisoquinoline Alkaloids ◽  
Isolation And Characterization ◽  
Thalictrum Minus

scholarly journals Short communication: Characterization of Staphylococcus aureus from bulk tank milk of dairy cattle in Lombardy (northern Italy)

2020 ◽  
Vol 103 (3) ◽  
pp. 2685-2692
Author(s):  
A. Gazzola ◽  
A.M. Maisano ◽  
V. Bianchini ◽  
F. Vezzoli ◽  
A. Romanò ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cattle ◽  
Short Communication ◽  
Northern Italy ◽  
Bulk Tank Milk ◽  
Bulk Tank

scholarly journals Characterization of methicillin-resistant Staphylococcus aureus ST398 from cases of bovine mastitis

2010 ◽  
Vol 65 (4) ◽  
pp. 619-625 ◽  
Author(s):  
A. Fessler ◽  
C. Scott ◽  
K. Kadlec ◽  
R. Ehricht ◽  
S. Monecke ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bovine Mastitis ◽  
Methicillin Resistant
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