scholarly journals Detection of β-lactamase, blaZ and mecA in penicillin-resistant Staphylococcus aureus isolated from bovine mastitis in Garanhuns, Brazil

2021 ◽  
Vol 15 (2) ◽  
pp. 140-145
Author(s):  
Amanda Pereira Lucas ◽  
Andriele Renata Barbosa de Farias ◽  
Elizabete Cristina da Silva ◽  
Kleber Régis Santoro ◽  
Marcelo Mendonça ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cattle ◽  
Meca Gene ◽  
Resistance Mechanism ◽  
Bovine Mastitis ◽  
Duplex Pcr ◽  
Genetic Determinants ◽  
The City

There are few reports in the literature about genetic determinants of resistance to β-lactams in Staphylococcus aureusisolated from dairy cattle located in the municipality of Garanhuns, state of Pernambuco, Brazil. Thus, this study aimed to investigate the production of β-lactamase and the presence of the blaZ and mecA genes in penicillin-resistant S. aureus isolated from cases of subclinical bovine mastitis in the city of Garanhuns. Forty-six strains of penicillin-resistant S. aureus were evalu-ated using the nitrocefin disc test and duplex PCR. The results revealed that 45 strains (97.8%) were positive for β-lactamase production and 44 (95.7%) carried the blaZ gene. Among the latter, 43 (97.7%) were β-lactamase producers and only one (2.3%) was not. The mecA gene was not detected in any of the isolates investigated. The results suggest that enzymatic inacti-vation is the main β-lactam resistance mechanism expressed by S. aureus in the herds analyzed.

scholarly journals Mutation-Based Antibiotic Resistance Mechanism in Methicillin-Resistant Staphylococcus aureus Clinical Isolates

2021 ◽  
Vol 14 (5) ◽  
pp. 420
Author(s):  
Tanveer Ali ◽  
Abdul Basit ◽  
Asad Mustafa Karim ◽  
Jung-Hun Lee ◽  
Jeong-Ho Jeon ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Active Site ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Meca Gene ◽  
Resistance Mechanism ◽  
Ligand Docking ◽  
Clinical Samples ◽  
Allosteric Site ◽  
Methicillin Resistant

β-Lactam antibiotics target penicillin-binding proteins and inhibit the synthesis of peptidoglycan, a crucial step in cell wall biosynthesis. Staphylococcus aureus acquires resistance against β-lactam antibiotics by producing a penicillin-binding protein 2a (PBP2a), encoded by the mecA gene. PBP2a participates in peptidoglycan biosynthesis and exhibits a poor affinity towards β-lactam antibiotics. The current study was performed to determine the diversity and the role of missense mutations of PBP2a in the antibiotic resistance mechanism. The methicillin-resistant Staphylococcus aureus (MRSA) isolates from clinical samples were identified using phenotypic and genotypic techniques. The highest frequency (60%, 18 out of 30) of MRSA was observed in wound specimens. Sequence variation analysis of the mecA gene showed four amino acid substitutions (i.e., E239K, E239R, G246E, and E447K). The E239R mutation was found to be novel. The protein-ligand docking results showed that the E239R mutation in the allosteric site of PBP2a induces conformational changes in the active site and, thus, hinders its interaction with cefoxitin. Therefore, the present report indicates that mutation in the allosteric site of PBP2a provides a more closed active site conformation than wide-type PBP2a and then causes the high-level resistance to cefoxitin.

scholarly journals Phenotypic and Genotypic Characterization of Beta-lactams Resistant Staphylococcus aureus Isolates from Bovine Mastitis and its Zoonotic Implications

2020 ◽  
Vol 40 (04) ◽  
pp. 523-526
Author(s):  
Ammara Khan
Keyword(s):  
Staphylococcus Aureus ◽  
Global Health ◽  
Genetic Relatedness ◽  
Meca Gene ◽  
Bovine Mastitis ◽  
Nasal Carriage ◽  
Pcr Analysis ◽  
Phenotypic Profile ◽  
Antimicrobial Resistance Genes ◽  
High Degree

Rise of antibiotic resistant Staphylococcus aureus is a higher risk and great concern to global health. Zoonotic transfer of such strains is well documented. Present study evaluated the presence of resistant S. aureus from mastitic Nilli Ravi buffaloes and nasal carriage of milkers. Phenotypic profile of S. aureus isolates was conducted against penicillin, ampicillin, and cefoxitin. PCR analysis revealed presence of blaZ gene and mecA gene from S. aureus isolated from milk and milkers samples. Sequence and phylogenetic analysis depicts the divergence of mecA gene originated from bovine and human but for the blaZ gene, no divergence was detected. The high degree of genetic relatedness among blaZ and mecA genes in bovine and human S. aureus isolates from same farm suggests the potential transfer of antimicrobial resistance genes between buffaloes and milkers, highlighting the importance of one-health approach to promote global health

Characterisation of mecA gene negative Staphylococcus aureus isolated from bovine mastitis milk from Northern Germany

2019 ◽  
Vol 64 (6) ◽  
pp. 845-855
Author(s):  
O. H. Sheet ◽  
N. T. Grabowski ◽  
G. Klein ◽  
F. Reich ◽  
A. Abdulmawjood
Keyword(s):  
Staphylococcus Aureus ◽  
Meca Gene ◽  
Bovine Mastitis ◽  
Northern Germany

scholarly journals Antimicrobial Susceptibility of Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli Isolated from Mastitic Dairy Cattle in Ukraine

Antibiotics ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 469
Author(s):  
Leta Elias ◽  
Ajay S. Balasubramanyam ◽  
Olena Y. Ayshpur ◽  
Iryna U. Mushtuk ◽  
Nataliya O. Sheremet ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Dairy Cattle ◽  
Antimicrobial Susceptibility ◽  
Streptococcus Agalactiae ◽  
Bovine Mastitis ◽  
Animal Husbandry ◽  
Economic Losses ◽  
Susceptibility Profiles

Bovine mastitis is the predominant cause for antimicrobial use on dairy farms and is a major source of economic losses in the dairy industry. In this study, the antimicrobial susceptibility profiles of common mastitis-causing pathogens, Staphylococcus aureus (n = 62), Streptococcus agalactiae (n = 46), and Escherichia coli (n = 129), were determined for dairy cattle with mastitis across 142 Ukrainian farms. The results showed that there were more gentamicin resistant S. aureus isolates (16.95%) identified in this study than previously reported for Ukrainian dairy cattle. Moreover, low levels of amoxicillin susceptibly (13.51%) were observed for St. agalactiae, which contrasted a previous study showing susceptibility levels of >50%. St. agalactiae resistance to tetracycline was observed in 80% of the isolates. Cephalosporin use was most ineffective against E. coli, with 43.27–56% of the isolates exhibiting this resistant trait. Overall, this study performed a preliminary analysis of antimicrobial resistance on mastitis isolates from Ukrainian farms. However, given the limited numbers of the isolates tested in this study and that the publications on antimicrobial resistance in animal husbandry of Ukraine are very few, more extensive investigations are needed to comprehensively examine susceptibility patterns of mastitis-causing pathogens in dairy cattle in Ukraine.

scholarly journals First mecC and mecA Positive Livestock-Associated Methicillin Resistant Staphylococcus aureus (mecC MRSA/LA-MRSA) from Dairy Cattle in Malaysia

2020 ◽  
Vol 8 (2) ◽  
pp. 147 ◽  
Author(s):  
Erkihun Aklilu ◽  
Hui Ying Chia
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cattle ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Meca Gene ◽  
Pcr Amplification ◽  
Diffusion Method ◽  
Methicillin Resistant ◽  
Unpasteurized Milk ◽  
Antimicrobial Susceptibility Tests ◽  
Pcr Targeting

Livestock associated Methicillin resistant Staphylococcus aureus (S. aureus) (LA-MRSA) was reported to be zoonotic and may transmit to farmers and veterinarians. The objectives of this study were to investigate the occurrence of LA-MRSA from dairy cattle and to evaluate the antimicrobial resistance profiles of the isolates. A total of 63 milk and 32 nasal swab samples were randomly collected from dairy cattle. The samples were processed to isolate S. aureus, MRSA and LA-MRSA using both phenotypic and molecular methods using PCR. The confirmed S. aureus isolates were cultured on oxacillin resistant screening agar base (ORSAB) to detect MRSA and the isolates were further confirmed by PCR targeting the mecA gene. Detection of the novel mecA gene, mecC gene was conducted by PCR amplification. The antimicrobial susceptibility tests were conducted using disc diffusion method. Results revealed 17/95 (17.89%) and 15/95 (15.79%) were positive for mecA and mecC genes respectively. Out of the 15 mecC positive isolates, 12 were positive for both mecA and mecC. The MRSA isolates showed multidrug resistance. The findings showed high prevalence of mecC-positive LA-MRSA in Malaysia and highlight the public health risks to people that may come in contact with the carrier animals or those who may consume unpasteurized milk products from these animals.

scholarly journals An overview: the virulence capacity, resistance mechanism, integron association, biofilm formation ability, and methicillin- resistance traits among staphylococcus aureus

2020 ◽  
Vol 11 (2) ◽  
pp. 23-41
Author(s):  
Anas Almousawi ◽  
Abdullah Alhatami ◽  
Furkan Alaraji
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Biofilm Formation ◽  
Virulence Genes ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Resistance Mechanism ◽  
Health Institutions ◽  
Animal Communities ◽  
Resistance Traits

The ability of Staphylococcus aureus (S. aureus) to acquire variety and many virulence genes that leads to its the ability to cause different diseases in many hosts species, these bacteria have many mechanisms for antibiotic resistance and their ability to produce biofilm and gain various genes through integrons, that will lead to difficult treatment. Consequently, the acquisition of the mecA gene made it more virulent and resistant to antibiotics, and this indicates that the spread of these bacteria in human and animal communities besides health institutions and their frequent transmission between these communities may have a problem of dealing with it.

scholarly journals Multilocus sequence typing of Staphylococcus aureus isolates recovered from cows with mastitis in Brazilian dairy herds

2007 ◽  
Vol 56 (11) ◽  
pp. 1505-1511 ◽  
Author(s):  
Renata F. Rabello ◽  
Beatriz M. Moreira ◽  
Regina M. M. Lopes ◽  
Lúcia M. Teixeira ◽  
Lee W. Riley ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Infectious Diseases ◽  
Dairy Cattle ◽  
Multilocus Sequence Typing ◽  
Bovine Mastitis ◽  
Control Measures ◽  
Dairy Herds ◽  
The South ◽  
Cattle Herds ◽  
Mastitis Control

Staphylococcus aureus is a major pathogen associated with bovine mastitis, one of the most important infectious diseases occurring in dairy cattle herds worldwide. In the present study, S. aureus isolates recovered from cows with mastitis in dairy herds located in the south-east of Brazil were genotyped by PFGE and multilocus sequence typing (MLST). PFGE identified 60 pulsotypes (PTs), which were found to be distributed among six clonal complexes (CCs) by MLST. All PTs with similarity percentages greater than 65 % belonged to the same CC. Most of the PTs belonged to CC126 (n=28) and CC97 (n=19), which were represented by 91 % of the isolates. These CCs have also been recovered from cows with mastitis in countries located in different continents, but they have rarely been isolated from human specimens. Few isolates were represented by PTs belonging to CCs that are frequently isolated from human specimens (CC1, CC5 and CC30). These data reinforce the hypothesis that a limited number of S. aureus CCs are responsible for most bovine mastitis cases internationally. Specific features of the specialized clones should be studied for use as future targets of mastitis control measures.

Assessment of the Multifactorial Effect on Antimicrobial Sensitivity in Positive Staphylococcus Aureus Clinical Isolates from Assir Region, Saudi Arabia

2012 ◽  
Vol 13 (2) ◽  
pp. 152-159 ◽  
Author(s):  
Nazar M Abdalla ◽  
Waleed O Haimour ◽  
Amani A Osman ◽  
Hassan Abdul Aziz
Keyword(s):  
Staphylococcus Aureus ◽  
Saudi Arabia ◽  
Culture Media ◽  
Meca Gene ◽  
Laboratory Data ◽  
Age Groups ◽  
Clinical Isolates ◽  
Diabetic Patients ◽  
Staph Aureus ◽  
Antimicrobial Sensitivity

General objectives: This study aimed at assessment of factors affecting antimicrobial sensitivity in Staphylococcus aureus clinical isolates from Assir region, Saudi Arabia. Materials and Methods: In this study, eighty one patients presented with Staph. aureus infections either nosocomial or community acquired infections were involved by collecting nasal swabs from them at Aseer Central Hospital General Lab. These patients were from all age groups and from males and females during the period of Jan 2011- Jun 2011. These samples were undergone variable laboratory procedures mainly; bactech, culture media, antibiotics sensitivity test using diffusion disc test (MIC) and molecular (PCR) for detection of mec A gene. Clinical and laboratory data were recorded in special formats and analyzed by statistical computer program (SPSS). Results: Showed that; Descriptive and analytical statistical analysis were performed and final results were plotted in tables. In Staph aureus MecA gene positive cases (50) showed: Oxacillin/ Mithicillin, Ciprofloxacin and Fusidin resistant in diabetic patients were 13, 26.0%, 9, 18% and 7, 14% respectively and in non diabetic patients were 37, 74.0%, 22, 44% and 20, 40% respectively. While no sensitivity in diabetic and non diabetic patients using Oxacillin/ Mithicillin. In Staph aureus MecA gene negative cases (31) showed: Oxacillin/ Mithicillin, sensitivity in diabetic patients (5, 16.1%) and in non diabetic were (26, 83.9%). While no resistant in diabetic and non diabetic patients. In Ciprofloxacin and Fusidin resistant in diabetic patients were 1, 3.2% and 1, 3.2% respectively and in non diabetic patients were 12, 38.7% and 7, 22.6%respectively. Erythromycin in Staph aureus ( MecA gene) positive cases (50) showed: resistant in age (0-15) years were (5, 10%), (16-50) years were (16, 32%) and ( ›50 years) were (12, 24%). Erythromycin in Staph aureus (MecA gene) negative cases (31) showed: resistant in age (0-15) years were (6, 19.3%), (16-50) years were (5, 16.1%) and ( ›50 years) were (3, 9.7%). Conclusion: Drugs resistance is a major progressive multifactorial problem facing the treatment of Staph aureus infections. DOI: http://dx.doi.org/10.3329/jom.v13i2.12750 J Medicine 2012; 13 : 152-159

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