Isolation and characterization of three benzylisoquinoline alkaloids from Thalictrum minus L. and their antibacterial activity against bovine mastitis

2016 ◽  
Vol 193 ◽  
pp. 221-226 ◽  
Author(s):  
Saleem Mushtaq ◽  
Muzafar Ahmad Rather ◽  
Parvaiz H. Qazi ◽  
Mushtaq A. Aga ◽  
Aabid Manzoor Shah ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bovine Mastitis ◽  
Benzylisoquinoline Alkaloids ◽  
Isolation And Characterization ◽  
Thalictrum Minus

Isolation and characterization of two new Staphylococcus aureus bacteriophages with potential to infect distinct bacteria from bovine mastitis

2021 ◽  
Author(s):  
Bibiana Martins Barasuol ◽  
Juliana Felipetto Cargnelutti ◽  
Luis Antônio Sangioni ◽  
Daniela Isabel Brayer Pereira ◽  
Ana Paula Muterle Varela ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bovine Milk ◽  
Bovine Mastitis ◽  
Gc Content ◽  
Etiologic Agent ◽  
Cure Rate ◽  
Long Tail ◽  
Isolation And Characterization ◽  
Double Stranded Dna

Abstract Bovine mastitis is an important disease of dairy cows, and Staphylococcus aureus is the etiologic agent most prevalent among the microorganisms. Mastitis caused by S. aureus present low cure rate with antimicrobials treatment and low vaccines efficacy. Bacteriophages or phages have been considered as an alternative for treating this disease. This study, we isolated and characterized two new S. aureus phages, namely B_UFSM4 and B_UFSM5, from bovine milk of cows with mastitis. The adsorptions rates were 10–20 min for B_UFSM4 and 20–30 min for B_UFSM5. Phages activities were relatively stable at pH 3–11; however, at temperatures of 50 °C-60ºC-70ºC/60 min, the phages were completely inactivated. These viruses presented infectivity in various bacteria isolated from bovine mastitis, where the lytic activity of phages B_UFSM4 and B_UFSM5 were 34.2%(13/38) and 42.1%(16/38), respectively, including isolates from S. aureus, Pseudomonas aeruginosa, Staphylococcus sciuri, and Rothia terrae. The complete genomes of B_UFSM4 and B_UFSM5 have 41.396 bp and 41.829 bp, with GC-content 33.97% and 33.98%, respectively. Both phages comprise 61 putative ORFs. The viruses have double stranded DNA and linear architecture. Phylogenic similarity was observed by proteome with Staphylococcus prophage phiPV83 (45,536 nt), Staphylococcus phage CN125 (44,492 nt) and Staphylococcus phage JS01 (43,458 nt). Based on the morphology, the phages belong to Siphoviridae family, presenting icosahedral head with a long tail, Caudovirales order and Biseptimavirus genus. Thus, two S. aureus phages (B_UFSM4 and B_UFSM5) were isolated and characterized, and these phages can be used as therapeutic or prophylactic candidates against S. aureus infections in cattle mastitis.

scholarly journals Isolation, characterization and biological properties of betulin from Entada africana Guill. and Perr. (Mimosaceae).

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
A. Kwaji ◽  
H. M. Adamu ◽  
I. Y. Chindo ◽  
R. Atiko
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Biological Properties ◽  
Pure Compound ◽  
Isolation And Characterization ◽  
Isolated Compound

The present study is aimed at the isolation and characterization of betulin from Entada africana.  A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.

Isolation and Characterization of Alkaloids from a New Chemotype of Thalictrum minus

Planta Medica ◽  
1980 ◽  
Vol 39 (05) ◽  
pp. 77-80 ◽  
Author(s):  
H. Dutschewska ◽  
B. Dimov ◽  
N. Mollov ◽  
L. Evstatieva
Keyword(s):  
Isolation And Characterization ◽  
Thalictrum Minus

scholarly journals Isolation and Characterization of Phenolic Compounds and Anthocyanins from Murta (Ugni molinae Turcz.) Fruits. Assessment of Antioxidant and Antibacterial Activity

Molecules ◽  
2015 ◽  
Vol 20 (4) ◽  
pp. 5698-5713 ◽  
Author(s):  
Maria Junqueira-Gonçalves ◽  
Lina Yáñez ◽  
Carolina Morales ◽  
Muriel Navarro ◽  
Rodrigo A. Contreras ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Isolation And Characterization ◽  
Ugni Molinae

scholarly journals Isolation, characterization and biological properties of betulin from Entada africana Guill. and Perr. (Mimosaceae).

2018 ◽  
Vol 3 (2) ◽  
pp. 28 ◽  
Author(s):  
A. Kwaji ◽  
H. M. Adamu ◽  
I. Y. Chindo ◽  
R. Atiko
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Biological Properties ◽  
Pure Compound ◽  
Isolation And Characterization ◽  
Isolated Compound

The present study is aimed at the isolation and characterization of betulin from Entada africana.  A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.

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