scholarly journals The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows

2021 ◽  
Author(s):  
Jacek Wawrzykowski ◽  
Monika Jamioł ◽  
Wioleta Mojsym ◽  
Marta Kankofer
Keyword(s):  
Blood Plasma ◽  
Antioxidative Enzymes ◽  
Placental Development ◽  
Blood Samples ◽  
Bovine Placenta ◽  
Total Antioxidant ◽  
Tissue Homogenates ◽  
Further Development ◽  
General Profile ◽  
In Blood Plasma

AbstractPhysiological balance between pro- and antioxidative processes is crucial for placentation and further development of fetus and placenta. Parameters of pro- and antioxidative profile may serve as markers of proper course of pregnancy. The aim of study was to assess whether the balance between pro- and antioxidative parameters during placentation phase in bovine placenta is maintained. Placental and blood samples were collected from healthy, HF, pregnant (2nd-3rd month) cows (n = 8) in slaughterhouse and in farm, respectively. Formylokinurenine and bityrosine content were measured spectrofluorimetrically in blood plasma and tissue homogenates while metabolites of lipid peroxidation, total antioxidant capacity, SH groups and activity of antioxidative enzymes (glutathione peroxidase and superoxide dismutase) were determined in examined tissues by spectrophotometry. Western blotting was used to confirm the presence of enzymatic proteins in placenta. Results: Local profile in tissues was more pronounced than general profile in blood plasma. Activities of antioxidative enzymes were significantly (p < 0.05) higher in 2nd compared to 3rd month of pregnancy in maternal part of placenta while prooxidant parameters showed opposite relationship. Obtained results showed significant differences when compared to data from non-pregnant animals or time of parturition. Further studies are necessary for elucidation of placentation phase in cows.

Detoxification of nitrosamides and nitrosocarbamates in blood plasma and tissue homogenates

1984 ◽  
Vol 6 (6) ◽  
pp. 835-849 ◽  
Author(s):  
Sharon Lea Aukerman ◽  
Robert B. Brundrett ◽  
Philip E. Hartman
Keyword(s):  
Blood Plasma ◽  
Tissue Homogenates ◽  
In Blood Plasma

scholarly journals The presence of ochratoxin A residue in blood plasma of slaughtered swine

2007 ◽  
pp. 55-62 ◽  
Author(s):  
Dragan Milicevic ◽  
Verica Juric ◽  
Miodrag Mandic ◽  
Milovan Djordjevic
Keyword(s):  
Blood Plasma ◽  
Ochratoxin A ◽  
Plasma Samples ◽  
Sample Analysis ◽  
Feed Material ◽  
Blood Samples ◽  
Spectrofluorimetric Method ◽  
Investigation Period ◽  
And Task ◽  
In Blood Plasma

The aim and task of this study was to determine the presence of ochratoxin A (OTA) residue in blood of swine, slaughtered regularly. The fact that ochratoxin A is heterogeneously distributed in a contaminated lot of feed material, makes the sampling problematic. It has been shown that an alternative method to monitor the presence of ochratoxin A in the feed is to analyze blood samples from swine, which reflect the toxin content of the ingested feed. With the aim of determining the presence of ochratoxin A residue in blood of swine slaughtered regularly, and originating from different areas of Vojvodina and Serbia, the samples were collected from the corresponding slaughter. During a three month investigation period, a total of 60 blood samples were analyzed. Spectrofluorimetric method was applied for sample analysis. The presence of the OTA residue was found in 56,6% of the examined plasma samples. The average OTA concentration in plasma was 2.91 ? 4.91 ng/mL (0,0-33,3 ng/mL). The experiment showed that the average OTA concentration in plasma samples originating from different areas of Vojvodina and Serbia, was not significantly low (p > 0,05).

State of hormonal regulation in experimental animals exposed to noise and vibration

2020 ◽  
pp. 680-681
Author(s):  
A. V. Lizarev ◽  
V. A. Pankov
Keyword(s):  
Blood Plasma ◽  
Hormonal Regulation ◽  
Adrenocorticotropic Hormone ◽  
Vibration Exposure ◽  
Noise And Vibration ◽  
Experimental Animals ◽  
In Blood Plasma

When exposed to noise and vibration in experimental animals there was a decrease in the content of threeiodinethyronine, thyroxin and adrenocorticotropic hormone in blood plasma after 15 and 30 days of experience. An increase in loads led to an increase in the level of threeiodinethyronine and thyroxin under vibration exposure and was normalized with noise. The content of adrenocorticotropic hormone leveled in both cases.

Comparison of Salivary Antioxidants and Oxidative Stress Status in Gestational Diabetes Mellitus and Healthy Pregnant Women

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Shima Fathi ◽  
Mohammad Taghi Goodarzi ◽  
Shiva Borzouei ◽  
Jalal Poorolajal ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Pregnant Women ◽  
Oxidative Stress Markers ◽  
Blood Samples ◽  
Stress Markers ◽  
Total Antioxidant ◽  
Total Oxidative Stress ◽  
And Oxidative Stress

Background: One of the most common complications of pregnant women is gestational diabetes mellitus (GDM). Oxidative stress can play an important role in GDM. Objective: The aim of this study was to evaluate salivary antioxidants and oxidative stress markers in GDM. Method: Twenty pregnant women with GDM and 20 healthy pregnant women with normal blood glucose test participated in this study. Five mL of unstimulated saliva samples were collected. Spectrophotometric assay was carried out for sialochemical analysis. Stata software was used for data analysis. Results: The GDM group exhibited no significant difference in salivary total antioxidant capacity and malondialdehyde compared to the healthy control group. All of antioxidants markers, the uric acid, total antioxidant, peroxidase and catalase, decreased in GDM group that the difference of peroxidase and catalase was statistically significant. All of oxidative stress markers, the salivary malondyaldehid, total oxidative stress and total thiol, increased in GDM group. GDM group exhibited significantly higher salivary total oxidative stress levels. Conclusion: Catalase level was significantly lower and total oxidative stress was significantly higher. These two markers might have significant importance and might exhibit early changes compared to other factors in GDM. . Some of salivary antioxidants might have diagnostic, prognostic or therapeutic implications in GDM. Other studies with large sample size on salivary and blood samples need to be done to confirm this properties and salivary samples using instead of blood samples in GDM biomarkers changes.

EFFECT OF DIETARY LEVELS OF SODIUM AND POTASSIUM ON GROWTH AND ON CONCENTRATIONS IN BLOOD PLASMA AND TISSUES OF WHITE RAT

1950 ◽  
Vol 162 (1) ◽  
pp. 182-188 ◽  
Author(s):  
J. H. Meyer ◽  
R. R. Grunert ◽  
Marie T. Zepplin ◽  
R. H. Grummer ◽  
G. Bohstedt ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Sodium And Potassium ◽  
In Blood Plasma

Two SPRi biosensors for the determination of cathepsin S in blood plasma

Talanta ◽  
2021 ◽  
Vol 225 ◽  
pp. 121900
Author(s):  
Lukasz Oldak ◽  
Anna Sankiewicz ◽  
Beata Żelazowska-Rutkowska ◽  
Bogdan Cylwik ◽  
Zenon Lukaszewski ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Cathepsin S ◽  
In Blood Plasma

scholarly journals DETERMINATION OF TRYPSIN INHIBITOR IN BLOOD PLASMA

1953 ◽  
Vol 204 (1) ◽  
pp. 147-152 ◽  
Author(s):  
Shirley F. McCann ◽  
M. Laskowski
Keyword(s):  
Blood Plasma ◽  
Trypsin Inhibitor ◽  
In Blood Plasma
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