The presence of ochratoxin A residue in blood plasma of slaughtered swine

The aim and task of this study was to determine the presence of ochratoxin A (OTA) residue in blood of swine, slaughtered regularly. The fact that ochratoxin A is heterogeneously distributed in a contaminated lot of feed material, makes the sampling problematic. It has been shown that an alternative method to monitor the presence of ochratoxin A in the feed is to analyze blood samples from swine, which reflect the toxin content of the ingested feed. With the aim of determining the presence of ochratoxin A residue in blood of swine slaughtered regularly, and originating from different areas of Vojvodina and Serbia, the samples were collected from the corresponding slaughter. During a three month investigation period, a total of 60 blood samples were analyzed. Spectrofluorimetric method was applied for sample analysis. The presence of the OTA residue was found in 56,6% of the examined plasma samples. The average OTA concentration in plasma was 2.91 ? 4.91 ng/mL (0,0-33,3 ng/mL). The experiment showed that the average OTA concentration in plasma samples originating from different areas of Vojvodina and Serbia, was not significantly low (p > 0,05).

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The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows

Molecular Biology Reports ◽

10.1007/s11033-021-06143-0 ◽

2021 ◽

Author(s):

Jacek Wawrzykowski ◽

Monika Jamioł ◽

Wioleta Mojsym ◽

Marta Kankofer

Keyword(s):

Blood Plasma ◽

Antioxidative Enzymes ◽

Placental Development ◽

Blood Samples ◽

Bovine Placenta ◽

Total Antioxidant ◽

Tissue Homogenates ◽

Further Development ◽

General Profile ◽

In Blood Plasma

AbstractPhysiological balance between pro- and antioxidative processes is crucial for placentation and further development of fetus and placenta. Parameters of pro- and antioxidative profile may serve as markers of proper course of pregnancy. The aim of study was to assess whether the balance between pro- and antioxidative parameters during placentation phase in bovine placenta is maintained. Placental and blood samples were collected from healthy, HF, pregnant (2nd-3rd month) cows (n = 8) in slaughterhouse and in farm, respectively. Formylokinurenine and bityrosine content were measured spectrofluorimetrically in blood plasma and tissue homogenates while metabolites of lipid peroxidation, total antioxidant capacity, SH groups and activity of antioxidative enzymes (glutathione peroxidase and superoxide dismutase) were determined in examined tissues by spectrophotometry. Western blotting was used to confirm the presence of enzymatic proteins in placenta. Results: Local profile in tissues was more pronounced than general profile in blood plasma. Activities of antioxidative enzymes were significantly (p < 0.05) higher in 2nd compared to 3rd month of pregnancy in maternal part of placenta while prooxidant parameters showed opposite relationship. Obtained results showed significant differences when compared to data from non-pregnant animals or time of parturition. Further studies are necessary for elucidation of placentation phase in cows.

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Ochratoxin A in human blood plasma samples from apparently healthy volunteers in Nanjing, China

Mycotoxin Research ◽

10.1007/s12550-020-00387-8 ◽

2020 ◽

Vol 36 (3) ◽

pp. 269-276 ◽

Cited By ~ 1

Author(s):

Kai Fan ◽

Xi Cheng ◽

Wenbo Guo ◽

Xing Liu ◽

Zhiqi Zhang ◽

...

Keyword(s):

Blood Plasma ◽

Healthy Volunteers ◽

Ochratoxin A ◽

Human Blood ◽

Human Blood Plasma ◽

Plasma Samples ◽

Apparently Healthy

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Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Journal of Visualized Experiments ◽

10.3791/61449 ◽

2020 ◽

Author(s):

Vikrant Palande ◽

Dorith Raviv Shay ◽

MILANA Frenkel-Morgenstern

Keyword(s):

Blood Plasma ◽

Cancer Patients ◽

Plasma Samples ◽

Cell Free Dna ◽

Free Dna ◽

In Blood Plasma

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Comparison of Genetic Alterations Detected in Circulating Microsatellite DNA in Blood Plasma Samples of Patients with Prostate Cancer and Benign Prostatic Hyperplasia

Annals of the New York Academy of Sciences ◽

10.1196/annals.1368.030 ◽

2006 ◽

Vol 1075 (1) ◽

pp. 222-229 ◽

Cited By ~ 28

Author(s):

I. MULLER ◽

K. URBAN ◽

K. PANTEL ◽

H. SCHWARZENBACH

Keyword(s):

Prostate Cancer ◽

Benign Prostatic Hyperplasia ◽

Blood Plasma ◽

Microsatellite Dna ◽

Genetic Alterations ◽

Prostatic Hyperplasia ◽

Plasma Samples ◽

In Blood Plasma

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Biomonitoring of ochratoxin A in blood plasma and exposure assessment of adult students in Bangladesh

Molecular Nutrition & Food Research ◽

10.1002/mnfr.201400403 ◽

2014 ◽

Vol 58 (11) ◽

pp. 2219-2225 ◽

Cited By ~ 22

Author(s):

Nurshad Ali ◽

Meinolf Blaszkewicz ◽

M. Manirujjaman ◽

Rasheda Perveen ◽

Abdullah Al Nahid ◽

...

Keyword(s):

Blood Plasma ◽

Adult Students ◽

Exposure Assessment ◽

Ochratoxin A ◽

In Blood Plasma

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Determination of Ascorbic Acid and Dehydroascorbic Acid in Blood Plasma Samples

Journal of Liquid Chromatography ◽

10.1080/01483918308080001 ◽

1983 ◽

Vol 6 (7) ◽

pp. 1319-1332 ◽

Cited By ~ 22

Author(s):

John K. Baker ◽

John Kapeghian ◽

Anthony Verlangieri

Keyword(s):

Ascorbic Acid ◽

Blood Plasma ◽

Dehydroascorbic Acid ◽

Plasma Samples ◽

In Blood Plasma

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Annealed ZnO/Al2O3 Core-Shell Nanowire as a Platform to Capture RNA in Blood Plasma

Nanomaterials ◽

10.3390/nano11071768 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1768

Author(s):

Hiromi Takahashi ◽

Takao Yasui ◽

Annop Klamchuen ◽

Narathon Khemasiri ◽

Tuksadon Wuthikhun ◽

...

Keyword(s):

Blood Plasma ◽

Molecular Detection ◽

High Efficiency ◽

Protective Layer ◽

Core Shell ◽

Plasma Samples ◽

Circulating Nucleic Acids ◽

Nanowire Device ◽

Analytical Platforms ◽

In Blood Plasma

RNA analytical platforms gained extensive attention recently for RNA-based molecular analysis. However, the major challenge for analyzing RNAs is their low concentration in blood plasma samples, hindering the use of RNAs for diagnostics. Platforms that can enrich RNAs are essential to enhance molecular detection. Here, we developed the annealed ZnO/Al2O3 core-shell nanowire device as a platform to capture RNAs. We showed that the annealed ZnO/Al2O3 core-shell nanowire could capture RNAs with high efficiency compared to that of other circulating nucleic acids, including genomic DNA (gDNA) and cell-free DNA (cfDNA). Moreover, the nanowire was considered to be biocompatible with blood plasma samples due to the crystalline structure of the Al2O3 shell which serves as a protective layer to prevent nanowire degradation. Our developed device has the potential to be a platform for RNA-based extraction and detection.

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