Assessment of genetic diversity of pumpkins (Cucurbita spp.) from western Kenya using SSR molecular markers

2021 ◽  
Vol 48 (3) ◽  
pp. 2253-2260
Author(s):  
Lameck A. Nyabera ◽  
Inosters W. Nzuki ◽  
Steven M. Runo ◽  
Peris W. Amwayi
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Western Kenya

Genetic Diversity of Basil (Ocimum spp. - Lamiaceae) Based on RAPD Markers and Volatile Oil Constituents

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 492f-493
Author(s):  
Roberto F. Vieira ◽  
James E. Simon ◽  
Peter Goldsbrough ◽  
Antonio Figueira
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Volatile Oil ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Traditional Medicines ◽  
The Tropics ◽  
High Degree ◽  
First Time

Essential oils extracted from basil (Ocimum spp.) by steam distillation are used to flavor foods, oral products, in fragrances, and in traditional medicines. The genus Ocimum contains around 30 species native to the tropics and subtropics, with some species naturalized and/or cultivated in temperate areas. Interand intraspecific hybridization have created significant confusion in the botanical systematics of this genus. Taxonomy of basil (O. basilicum) is also complicated by the existence of numerous varieties, cultivars, and chemotypes within the species that do not differ significantly in morphology. In this study we are using RAPD markers and volatile oil composition to characterize the genetic diversity among the most economically important Ocimum species. We hypothesize that the genetic similarity revealed by molecular markers will more accurately reflect the morphological and chemical differences in Ocimum than essential oil composition per se. Preliminary research using five Ocimum species, four undetermined species, and eight varieties of O. basilicum (a total of 19 accessions) generated 107 polymorphic fragments amplified with 19 primers. RAPDs are able to discriminate between Ocimum species, but show a high degree of similarity between O. basilicum varieties. The genetic distance between nine species and among 55 accessions within the species O. americanum, O. basilicum, O. campechianum, O. × citriodorum, O. gratissimum, O. kilimandscharium, O. minimum, O. selloi, and O. tenuiflorum will be analyzed by matrix of similarity and compared to the volatile oil profile. This research will for the first time apply molecular markers to characterize the genetic diversity of Ocimum associate with volatile oil constituent.

Molecular markers and antioxidant activity in berry crops: Genetic diversity analysis

2012 ◽  
Vol 92 (6) ◽  
pp. 1121-1133 ◽  
Author(s):  
S. C. Debnath ◽  
Y. L. Siow ◽  
J. Petkau ◽  
D. An ◽  
N. V. Bykova
Keyword(s):  
Genetic Diversity ◽  
Antioxidant Activity ◽  
Molecular Markers ◽  
Molecular Techniques ◽  
Diversity Analysis ◽  
Full Potential ◽  
Genetic Diversity Analysis ◽  
Wide Range ◽  
Berry Crops ◽  
Improvement Programs

Debnath, S. C., Siow, Y. L., Petkau, J., An, D. and Bykova, N. V. 2012. Molecular markers and antioxidant activity in berry crops: Genetic diversity analysis. Can. J. Plant Sci. 92: 1121–1133. An improved understanding of important roles of dietary fruits in maintaining human health has led to a dramatic increase of global berry crop production. Berry fruits contain relatively high levels of vitamin C, cellulose and pectin, and produce anthocyanins, which have important therapeutic values, including antitumor, antiulcer, antioxidant and anti-inflammatory activities. There is a need to develop reliable methods to identify berry germplasm and assess genetic diversity/relatedness for dietary properties in berry genotypes for practical breeding purposes through genotype selection in a breeding program for cultivar development, and proprietary-rights protection. The introduction of molecular biology techniques, such as DNA-based markers, allows direct comparison of different genetic materials independent of environmental influences. Significant progress has been made in diversity analysis of wild cranberry, lowbush blueberry, lingonberry and cloudberry germplasm, and in strawberry and raspberry cultivars and advanced breeding lines developed in Canada. Inter simple sequence repeat (ISSR) markers detected an adequate degree of polymorphism to differentiate among berry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in the current berry improvement programs. Although multiple factors affect antioxidant activity, a wide range of genetic diversity has been reported in wild and cultivated berry crops. Diversity analysis based on molecular markers did not agree with those from antioxidant activity. The paper also discusses the issues that still need to be addressed to utilize the full potential of molecular techniques including expressed sequence tag-polymerase chain reaction (EST-PCR) analysis to develop improved environment-friendly berry cultivars suited to the changing needs of growers and consumers.

Evaluation of genetic diversity among some genotypes of Kentucky bluegrass by RAPD molecular markers

2012 ◽  
Vol 53 (4) ◽  
pp. 298-303 ◽  
Author(s):  
Javad Rezapour Fard ◽  
Zabihollah Zamani ◽  
Mohamad Reza Fattahi Moghaddam ◽  
Mohsen Kafi
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Kentucky Bluegrass

scholarly journals ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth.

2016 ◽  
Vol 34 (3) ◽  
pp. 47-52 ◽  
Author(s):  
Fernando dos Santos Araújo ◽  
Mauro Vasconcelos Pacheco ◽  
Fábio de Almeida Vieira ◽  
Cibele dos Santos Ferrari ◽  
Francival Cardoso Félix ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Issr Molecular Markers

scholarly journals Genetic diversity in a Poincianella pyramidalis (Tul.) L.P. Queiroz population assessed by RAPD molecular markers

2017 ◽  
Vol 16 (3) ◽  
Author(s):  
K.S. Belarmino ◽  
M.M. Rêgo ◽  
R.L.A. Bruno ◽  
G.D.A. Medeiros ◽  
A.P. Andrade ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers

Chromosome Number, Genomic Variation, and Molecular Markers to Assess Genetic Diversity of Brachiaria Species

Crop Science ◽  
2016 ◽  
Vol 56 (1) ◽  
pp. 312-321 ◽  
Author(s):  
Pattama Nitthaisong ◽  
Genki Ishigaki ◽  
Hidenori Tanaka ◽  
Ryo Akashi
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Chromosome Number ◽  
Genomic Variation

Analysis of genetic diversity in Cassia brewsteri with randomly amplified DNA fingerprints (RAFs)

2002 ◽  
Vol 15 (2) ◽  
pp. 237 ◽  
Author(s):  
David C. Cunningham ◽  
Kerry B. Walsh ◽  
Eric R. Anderson ◽  
Dion K. Harrison ◽  
Bernie J. Carroll
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Multidimensional Scaling ◽  
Dna Fingerprints ◽  
Similarity Coefficients ◽  
Natural Distribution ◽  
Amplification Profile

Genetic diversity in Cassia brewsteri (F.Muell.) F.Muell. ex Benth. was assessed with Randomly Amplified DNA Fingerprints (RAFs). Thirty accessions of C. brewsteri collected from throughout its natural distribution were analysed with three random decamer primers, along with three accessions of C. tomentella (Benth.) Domin and a single accession of each of C. queenslandica C.T.White and C. marksiana (F.M.Bailey) Domin. The three primers yielded a reproducible amplification profile of 265 scorable polymorphic fragments for the 35 accessions. These molecular markers were used to calculate Nei and Li similarity coefficients between each pair of individuals. A matrix of dissimilarity of each pair of individuals was examined by multidimensional scaling (MDS). The analysis supports the division of C. brewsteri into two subspecies and the suggestion that intergradation ofC. brewsteri and C. tomentella can occur where the distributions of these species meet.

scholarly journals A multi-farm assessment of Greek black pig genetic diversity using microsatellite molecular markers

2014 ◽  
Vol 13 (2) ◽  
pp. 2752-2765 ◽  
Author(s):  
S. Michailidou ◽  
A. Kalivas ◽  
I. Ganopoulos ◽  
E. Stea ◽  
G. Michailidis ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers
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