Effect of Fonsecaea monophora on the Polarization of THP-1 Cells to Macrophages

ABSTRACT We have evaluated the efficacy of posaconazole, amphotericin B, and itraconazole in a murine model of disseminated infection by Fonsecaea monophora. Of these three antifungal drugs tested, posaconazole prolonged survival significantly and reduced the fungal load in most of the organs tested. Bioassay studies demonstrated the relationship between posaconazole levels and dose escalation in serum and brain tissue. Posaconazole may have a clinical role in the treatment of disseminated infections by F. monophora.

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Agrobacterium tumefaciens-Mediated Transformation of Fonsecaea monophora and Fonsecaea erecta for Host-Environment Interaction Studies

Journal of Fungi ◽

10.3390/jof6040325 ◽

2020 ◽

Vol 6 (4) ◽

pp. 325

Author(s):

Cristina Isabel Ferrer Villena ◽

Renata Rodrigues Gomes ◽

Larissa Fernandes ◽

Camille Silva Florencio ◽

Amanda Bombassaro ◽

...

Keyword(s):

Environment Interaction ◽

Bactris Gasipaes ◽

Invasive Potential ◽

Brain Infection ◽

Fonsecaea Monophora ◽

Confocal Fluorescence ◽

Causative Agents ◽

Potential Link ◽

Foreign Dna ◽

Etiological Agents

The fungal genus Fonsecaea contains etiological agents of human chromoblastomycosis, a (sub)tropical, (sub)cutaneous implantation disease caused by plant contact. The invasive potential differs significantly between species. Infections by Fonsecaea monophora are believed to originate from the environment and the species has been reported as one of the main causative agents of the disease, but also of cases of primary brain infection. The epidemiology of the disease has not been fully elucidated and questions related to its infection route and virulence are still to be clarified. The environmental species Fonsecaea erecta was isolated from organic material and living plants in endemic areas for chromoblastomycosis in Brazil. The present paper describes Agrobacteriumtumefaciens-mediated transformation (AMT) of the environmental species F. erecta and the pathogenic species F. monophora. We propose the use of Agrobacterium transformation for future gene function studies related to Fonsecaea virulence and pathogenicity. We evaluated the co-cultivation ratios 1:1, 10:1 and 100:1 (Agrobacterium:conidia) at 28 °C during 72 h. pAD1625 and pCAMDsRed plasmids were inserted into both species. Confirmation of transformation was realized by hph gene amplification and Southern blot determined the amount of foreign DNA integrated into the genome. In order to evaluate a potential link between environmental and clinical strains, we obtained red fluorescent transformants after pCAMDsRed insertion. We observed by confocal fluorescence microscopy that both F. monophora and F. erecta were able to colonize the palm Bactris gasipaes, penetrating the epidermis. These results contribute to understanding the ability of Fonsecaea species to adapt to different environmental and host conditions.

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A Case of Relapsed Chromoblastomycosis Due to Fonsecaea monophora: Antifungal Susceptibility and Phylogenetic Analysis

Mycopathologia ◽

10.1007/s11046-013-9660-1 ◽

2013 ◽

Vol 176 (1-2) ◽

pp. 139-144 ◽

Cited By ~ 9

Author(s):

Tatiane Caroline Daboit ◽

Cibele Massotti Magagnin ◽

Daiane Heidrich ◽

Mauricio Ramírez Castrillón ◽

Sandra Denise Camargo Mendes ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Antifungal Susceptibility ◽

Fonsecaea Monophora

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In Vitro Activities of Eight Antifungal Drugs against 55 Clinical Isolates of Fonsecaea spp.

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01655-09 ◽

2010 ◽

Vol 54 (4) ◽

pp. 1636-1638 ◽

Cited By ~ 39

Author(s):

Mohammad J. Najafzadeh ◽

Hamid Badali ◽

Maria Teresa Illnait-Zaragozi ◽

G. Sybren De Hoog ◽

Jacques F. Meis

Keyword(s):

Amphotericin B ◽

Antifungal Drugs ◽

Clinical Isolates ◽

Fonsecaea Pedrosoi ◽

Fonsecaea Monophora

ABSTRACT The in vitro activities of eight antifungal drugs against clinical isolates of Fonsecaea pedrosoi (n = 21), Fonsecaea monophora (n = 25), and Fonsecaea nubica (n = 9) were tested. The resulting MIC90s for all strains (n = 55) were as follows, in increasing order: posaconazole, 0.063 μg/ml; itraconazole, 0.125 μg/ml; isavuconazole, 0.25 μg/ml; voriconazole, 0.5 μg/ml; amphotericin B, 2 μg/ml; caspofungin, 2 μg/ml; anidulafungin, 2 μg/ml; and fluconazole, 32 μg/ml.

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