Method for quantitative determination of various forms of water in biologically active substances

2011 ◽  
Vol 44 (10) ◽  
pp. 574-580
Author(s):  
B. N. Boiko ◽  
I. M. Kolpakov ◽  
A. A. Uminskii
Keyword(s):  
Quantitative Determination ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Active Substances

scholarly journals Flavonoid’s Content Determination in Irradiated Samples via Spectrophotometric Analysis

2019 ◽  
Vol 5 (10) ◽  
pp. 32-39 ◽  
Author(s):  
A. Sarukhanov ◽  
A. Morozova ◽  
N. Vasilyeva ◽  
D. Krylenkin
Keyword(s):  
Quantitative Determination ◽  
Raw Materials ◽  
General Information ◽  
Spectrophotometric Analysis ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Qualitative And Quantitative ◽  
Content Determination ◽  
Active Substances

General information about biologically active substances flavonoids is concisely presented. The need for the quantitative determination of its content in products and the influence of product processing on flavonoid’s content is justified. Methods of qualitative and quantitative determination of flavonoids content in various products, medications and raw materials were studied. Based on this study the method of quantitative determination of flavonoid’s in samples of irradiated coriander is compiled and tested. Further ways to improve and adjust the compiled method for flavonoid’s content determination in irradiated samples via spectrophotometric analysis is considered.

SELECTION OF COMPOSITION AND TECHNOLOGY OF GRANULES WITH GARDEN LEAVE STRAWBERRY EXTRACT DRY

2021 ◽  
pp. 154-161
Author(s):  
Yaborova O.V. ◽  
Zamakhaeva E.A. ◽  
Oleshko O.A.
Keyword(s):  
Quantitative Determination ◽  
Raw Materials ◽  
Food Product ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
Leaf Extracts ◽  
Optimal Method ◽  
Active Substances

Garden strawberries (Fragaria ananassa) are widely cultivated on the territory of Russia on industrial plantations and private garden plots to obtain the fruits (berries) of this plant and their use as a valuable food product. When harvesting fruits, other parts of the garden strawberry are not used, however, its vegetative parts are a source of biologically active substances (BAS) and are of interest for the pharmaceutical and cosmetic industries [1, 4]. The biological activity of garden strawberry leaf extracts is due to the presence of secondary metabolites of a phenolic nature; their content in raw materials reaches more than 3% in some samples [2, 3]. Considering that the main group of biologically active substances of this raw material are flavonoids, the Department of Pharmacognosy with a course of botany conducted research to develop a method for quantitative determination of the amount of flavonoids in terms of rutin. Spectrophotometry was chosen as the optimal method for assessing the content of this group of substances in medicinal plant raw materials - leaves of garden strawberries, which allows determining the amount of flavonoids in terms of the dominant compound and obtaining reliable and reproducible results of quantitative analysis [8, 11]. The technological properties were studied and a therapeutic dose of dry strawberry extract was proposed, optimal compositions were selected and technologies of sachet-granules with dry strawberry extract were developed. The method for the quantitative determination of flavonoids in terms of rutin was validated. The results of the study can be used in the future in the development of draft regulatory documents for sachet-granules with dry strawberry extract of garden leaves and the study of their pharmacological activity.

scholarly journals Phytochemical Analysis of Elaeagnus argentea Leaves

2021 ◽  
Vol 10 (3) ◽  
pp. 95-99
Author(s):  
N. A. Salnikova ◽  
Yu. V. Shur ◽  
A. A. Tsibizova
Keyword(s):  
Ascorbic Acid ◽  
Quantitative Determination ◽  
Raw Materials ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
Phytochemical Analysis ◽  
Promising Source ◽  
Active Substances

Introduction. Currently, close attention in the field of pharmacy and medicine is directed to the search for new sources of biologically active substances of various origins, including vegetable. The woody plant Elaeagnus argentea is considered as a promising source. Despite its widespread use in the food industry, folk medicine as an anti-inflammatory, restorative, antimicrobial agent, the chemical composition of this plant has not been sufficiently studied.Aim. Qualitative and quantitative determination of the main groups of biologically active substances (BAS) in the raw material (leaves) of Elaeagnus argentea growing on the territory of the Astrakhan region for further development of a methodology for standardizing the raw material of this plant.Materials and methods. The leaves of Elaeagnus argentea were harvested in the spring in the eastern part of the delta of the Astrakhan region (Volga region). Drying of raw materials was carried out in natural conditions. In the study, unified methods were used: to determine the amount of flavonoids and saponins, the spectrophotometric method was used, ascorbic acid - the titrimetric method. For the analysis of flavonoids in the leaves of Elaeagnus argentea, an aqueous-alcoholic solution of 70 % concentration was used as an extractant. The quantitative content of flavonoids was determined in the obtained raw material extract in terms of luteolin-7-glucoside. The amount of saponins in the leaves of Elaeagnus argentea was determined in terms of oleanolic acid. 96 % ethanol was used as an extractant. The quantitative determination of ascorbic acid in the aqueous extract of crushed raw materials was carried out by titrimetry based on the ability to reduce 2,6-dichlorophenolindophenol.Results and discussion. In the pharmacognostic study of biologically active substances in the leaves of Elaeagnus argentea, the content of ascorbic acid was found to be at least 0.32 %, the amount of flavonoids in terms of luteolin-7-glucoside was at least 1.92 %; saponins - 2.38 %; which indicates the need for a more detailed study of the phytochemical composition of other morphological groups of the plant Elaeagnus argentea growing in the Astrakhan region.Conclusion. The data obtained during the study can be used to confirm the quality of raw materials (leaves) of Elaeagnus argentea. A more detailed study of leaves for the presence of other groups of biologically active substances will make it possible to use the obtained data for the development of regulatory documents for medicinal plant raw materials «Elaeagnus argentea leaves».

scholarly journals SPECTROPHOTOMETRIC DETERMINATION OF ANTIOXIDANT SODIUM METABISULPHITE CONTENT IN PREPARED MEDICINAL FORMS OF VETERINARY MEDICINAL PRODUCTS

2020 ◽  
Vol 21 (2) ◽  
pp. 189-198
Author(s):  
H. Yu. Teslyar ◽  
M. Ya. Smolinska ◽  
I. Ya. Kotsyumbas ◽  
N. M. Chyhyn ◽  
N. G. Rohulia ◽  
...  
Keyword(s):  
Spectrophotometric Determination ◽  
Storage Time ◽  
Analytical Signal ◽  
Veterinary Drugs ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Optimal Conditions ◽  
Sodium Metabisulphite ◽  
Active Substances

An analytical method for the quantitative determination of antioxidant sodium metabisulphite in veterinary drugs has been proposed by spectrophotometric method. Based on the literature data, the optimal conditions of the analytical reaction were selected experimentally. The dependence of the value of the analytical signal on the temperature of the reaction medium, concentration of p-rosaniline and duration of the reaction was investigated to establish the optimal conditions of the analytical reaction and obtain a stable analytical signal. The stability of the colored analytical form in time was also checked and the linear dependence of the value of the analytical signal on the concentration of sodium metabisulphite was investigated. The analytical reaction at room temperature is optimal. The maximum analaytical signal is achieved by carrying out the analytical reaction for 10 minutes and then practically does not change for an hour. To achieve the maximum analytical signal, it is necessary to use a 20-fold excess of dye relative to sodium metabisulphite. The analytical signal remains stable only for the first hour, then gradually begins to decrease. Metrological characteristics of the method of determination of metabisulphite in veterinary drugs are calculated, the limits of spectrophotometric determination are 0.33 – 2.50 μg/ml. The correctness of the developed method was checked on model solutions by the method of "introduced-found" method of comparison in the presence of various biologically active substances that are part of drugs together with sodium metabisulphite. The content of sodium metabisulphite in veterinary drugs of domestic and foreign production at different intervals of their storage time was established. It is shown that the content of sodium metabisulphite in drugs decreases during their storage time, until complete disappearance, which directly affects the content of the active substance, because in the absence of antioxidant oxidative processes with biologically active substances begin to take place.

DETERMINATION OF BIOLOGICALLY ACTIVE SUBSTANCES – FLAVONOIDS IN FRUIT TREES WITH HEALING PROPERTIES

2020 ◽  
pp. 94-96
Author(s):  
P. Mirkhamidova ◽  
D. B. Babakhanova ◽  
G. I. Mukhamedov ◽  
R. A. Alimova
Keyword(s):  
Fruit Trees ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Active Substances

A kinetic determination of ultramicroquantities of some biologically active substances

1980 ◽  
Vol 25 (3) ◽  
pp. 380-387 ◽  
Author(s):  
G.A. Milovanović ◽  
T.J. Janjić ◽  
S. Petrović ◽  
G. Kuzmanović
Keyword(s):  
Biologically Active Substances ◽  
Biologically Active ◽  
Kinetic Determination ◽  
Active Substances
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