In Vitro Metabolism of 5-ALA Esters Derivatives in Hairless Mice Skin Homogenate and in Vivo PpIX Accumulation Studies

2004 ◽  
Vol 21 (12) ◽  
pp. 2247-2252 ◽  
Author(s):  
Fernanda Scarmato De Rosa ◽  
Renata Fonseca Vianna Lopez ◽  
Jos� Ant�nio Thomazine ◽  
Ant�nio Cl�udio Tedesco ◽  
Norbert Lange ◽  
...  
Keyword(s):  
In Vitro Metabolism ◽  
Hairless Mice ◽  
Skin Homogenate ◽  
Ala Esters

scholarly journals 5-Aminolevulinic Acid-Based Photodynamic Therapy Pretreatment Mitigates Ultraviolet A-Induced Oxidative Photodamage

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Hui Hua ◽  
Jiawei Cheng ◽  
Wenbo Bu ◽  
Juan Liu ◽  
Weiwei Ma ◽  
...  
Keyword(s):  
Aminolevulinic Acid ◽  
Epidermal Keratinocytes ◽  
Control Group ◽  
Hacat Cells ◽  
Ultraviolet A ◽  
Hairless Mice ◽  
Human Epidermal Keratinocytes ◽  
Experimental Group

Aim. To determine whether 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT) is effective in combating ultraviolet A- (UVA-) induced oxidative photodamage of hairless mice skin in vivo and human epidermal keratinocytes in vitro. Methods. In in vitro experiments, the human keratinocyte cell line (HaCaT cells) was divided into two groups: the experimental group was treated with ALA-PDT and the control group was left untreated. Then, the experimental group and the control group of cells were exposed to 10 J/m2 of UVA radiation. ROS, O2− species, and MMP were determined by fluorescence microscopy; p53, OGG1, and XPC were determined by Western blot analysis; apoptosis was determined by flow cytometry; and 8-oxo-dG was determined by immunofluorescence. Moreover, HaCaT cells were also treated with ALA-PDT. Then, SOD1 and SOD2 were examined by Western blot analysis. In in vivo experiments, the dorsal skin of hairless mice was treated with ALA-PDT or saline-PDT, and then, they were exposed to 20 J/m2 UVA light. The compound 8-oxo-dG was detected by immunofluorescence. Conclusion. In human epidermal keratinocytes and hairless mice skin, UVA-induced oxidative damage can be prevented effectively with ALA-PDT pretreatment.

In vivo and in vitro metabolism of the novel synthetic cannabinoid 5F-APINAC

2019 ◽  
Vol 38 (1) ◽  
pp. 160-171 ◽  
Author(s):  
Svetlana A. Appolonova ◽  
Covadonga Palacio ◽  
Ksenia M. Shestakova ◽  
Natalia V. Mesonzhnik ◽  
Alex Brito ◽  
...  
Keyword(s):  
Synthetic Cannabinoid ◽  
In Vitro Metabolism ◽  
The Novel

Enantioselective in vitro metabolism and in vitro-in vivo correlation of the herbicide ethofumesate in a human model

2020 ◽  
Vol 187 ◽  
pp. 113349 ◽  
Author(s):  
Icaro Salgado Perovani ◽  
Daniel Blascke Carrão ◽  
Nayara Cristina Perez de Albuquerque ◽  
Anderson Rodrigo Moraes de Oliveira
Keyword(s):  
In Vitro Metabolism ◽  
Human Model
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