Modulating Protein Release Profiles by Incorporating Hyaluronic Acid into PLGA Microparticles Via a Spray Dryer Equipped with a 3-Fluid Nozzle

Polymeric microparticles (MPs) designed for the intravitreal administration of therapeutic proteins result in a prolonged half-life in the vitreous and can delay or discourage the onset of adverse effects inevitably related to this route of administration. Hence, here we designed MPs composed of a polymeric blend based on poly(lactic-co-glycolic) acid and poloxamers, externally decorated with hyaluronic acid. The MPs are intended for intravitreal administration of bovine serum albumin. In detail, a systematic formulative study aiming to shed light on the complex relationship between protein release rate and MP degradation rate was carried out by means of calorimetric and gel permeation chromatography analyses. We found out that poloxamer addition caused a compact MP matrix, which led to a slight modification of the degradation kinetics and a reduction in the initial BSA initial release, which is of the utmost importance to ensure a relatively regular BSA release. It must also be underlined that for acid-labile molecules such as proteins, the poloxamer’s presence induced complex and hardly predictable effects on MP degradation/protein release, due to the dynamic balance between the time-evolving hydrophilicity of MPs and the influence of poloxamers themselves on the PLGA degradation rate.

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Protein release profiles and morphology of biodegradable microcapsules containing an oily core

Journal of Controlled Release ◽

10.1016/s0168-3659(01)00445-x ◽

2001 ◽

Vol 76 (3) ◽

pp. 313-326 ◽

Cited By ~ 44

Author(s):

Bi Botti C Youan ◽

Tanise L Jackson ◽

Lorenzo Dickens ◽

Carmen Hernandez ◽

Godfried Owusu-Ababio

Keyword(s):

Protein Release ◽

Release Profiles

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Biodegradable Hyaluronic Acid/N-Carboxyethyl Chitosan/Protein Ternary Complexes as Implantable Carriers for Controlled Protein Release

Macromolecular Bioscience ◽

10.1002/mabi.200500126 ◽

2005 ◽

Vol 5 (12) ◽

pp. 1226-1233 ◽

Cited By ~ 26

Author(s):

Hongliang Jiang ◽

Yijuan Wang ◽

Qian Huang ◽

Yan Li ◽

Chaonan Xu ◽

...

Keyword(s):

Hyaluronic Acid ◽

Ternary Complexes ◽

Protein Release

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Calcium ion modulates protein release from chitosan-hyaluronic acid poly electrolyte gel

Polymer Engineering & Science ◽

10.1002/pen.24050 ◽

2015 ◽

Vol 55 (9) ◽

pp. 2089-2097 ◽

Cited By ~ 4

Author(s):

A. Shanti Krishna ◽

C. Radhakumary ◽

K. Sreenivasan

Keyword(s):

Hyaluronic Acid ◽

Calcium Ion ◽

Protein Release

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Characterization of protein release from photocrosslinkable hyaluronic acid-polyethylene glycol hydrogel tissue engineering scaffolds

Biomaterials ◽

10.1016/j.biomaterials.2004.02.018 ◽

2005 ◽

Vol 26 (2) ◽

pp. 125-135 ◽

Cited By ~ 307

Author(s):

Jennie B Leach ◽

Christine E Schmidt

Keyword(s):

Tissue Engineering ◽

Hyaluronic Acid ◽

Polyethylene Glycol ◽

Protein Release ◽

Tissue Engineering Scaffolds

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The release profiles of intact and enzymatically digested hyaluronic acid from semisolid formulations using multi-layer membrane system

European Journal of Pharmaceutics and Biopharmaceutics ◽

10.1016/s0939-6411(03)00029-8 ◽

2003 ◽

Vol 56 (1) ◽

pp. 37-41 ◽

Cited By ~ 4

Author(s):

Jamal Alyoussef Alkrad ◽

Yahya Mrestani ◽

Reinhard H.H Neubert

Keyword(s):

Hyaluronic Acid ◽

Membrane System ◽

Layer Membrane ◽

Release Profiles

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ENCAPSULATION AND RELEASE PROFILE OF PROTEIN CAGE FROM A POLYMERIC MATRIX

Nano LIFE ◽

10.1142/s1793984411000347 ◽

2012 ◽

Vol 02 (01) ◽

pp. 1250001 ◽

Cited By ~ 2

Author(s):

YAN LI ◽

RIKE OKTAVIANTI TOYIP ◽

TAO PENG ◽

SIERIN LIM

Keyword(s):

Aqueous Phase ◽

Drug Carrier ◽

Polymer Concentration ◽

Ionic Concentration ◽

Release Profile ◽

Protein Release ◽

Cage Structure ◽

E2 Protein ◽

Microparticle Size ◽

Plga Microparticles

Protein cages have been widely investigated as molecular drug carrier. E2 protein from Bacillus stearothermophillus forms a dodecahedral cage structure of approximately 24 nm in diameter. To formulate a sustainable release profile, E2 protein was further encapsulated into poly(lactide-co-glycolide) (PLGA) microparticles to form a composite structure using water-in-oil-in-water (W/O/W) double emulsion method. The influence of fabrication parameters on microparticle morphology and E2 protein release profile were investigated. The microparticle size increased when the stirring speed of the second emulsification decreased. Decrease in the volume of external aqueous phase led to the reduction of microparticle size without affecting its porosity. The higher ionic concentration of external aqueous phase in the presence of surfactant resulted in microparticles with closed pores on surface. Increase in polymer concentration also led to the formation of less porous microparticles. The E2 protein was not dissociated upon encapsulation into PLGA microparticles based on the unchanged particle size of E2 protein. E2 protein release was studied in phosphate-buffered saline solution at 37°C. The initial burst and release rate were lowered as the surfactant concentration in external water phase during the fabrication process was increased from 0.1% to 1% (w/v). After 14-day incubation, no observable polymer degradation was found while the surface of microparticles appeared to be smoother than before incubation.

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