Formulations for modulation of protein release from large-size PLGA microparticles for tissue engineering

In this study, to improve the cellular interaction and protein release of gelatin hydrogels, we reported the development of a new hybrid hydrogel platform as a promising tissue engineering scaffold and drug delivery carrier.

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Microsphere-integrated collagen scaffolds for tissue engineering: Effect of microsphere formulation and scaffold properties on protein release kinetics

Journal of Controlled Release ◽

10.1016/j.jconrel.2006.04.011 ◽

2006 ◽

Vol 113 (2) ◽

pp. 128-136 ◽

Cited By ~ 77

Author(s):

Francesca Ungaro ◽

Marco Biondi ◽

Ivana d'Angelo ◽

Laura Indolfi ◽

Fabiana Quaglia ◽

...

Keyword(s):

Tissue Engineering ◽

Release Kinetics ◽

Protein Release ◽

Collagen Scaffolds ◽

Scaffold Properties

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Chitosan-based nanoparticles as a sustained protein release carrier for tissue engineering applications

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.34031 ◽

2012 ◽

Vol 100A (4) ◽

pp. 939-947 ◽

Cited By ~ 51

Author(s):

Yaping Hou ◽

Junli Hu ◽

Hyejin Park ◽

Min Lee

Keyword(s):

Tissue Engineering ◽

Protein Release ◽

Engineering Applications ◽

Sustained Protein Release

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Enhancing Kidney Vasculature in Tissue Engineering—Current Trends and Approaches: A Review

Biomimetics ◽

10.3390/biomimetics6020040 ◽

2021 ◽

Vol 6 (2) ◽

pp. 40

Author(s):

Charlotta G. Lebedenko ◽

Ipsita A. Banerjee

Keyword(s):

Tissue Engineering ◽

Large Scale ◽

Kidney Diseases ◽

Kidney Tissue ◽

Small Scale ◽

Current Trends ◽

Large Size ◽

Microphysiological Systems ◽

Primary Obstacle ◽

Kidney Stem Cells

Chronic kidney diseases are a leading cause of fatalities around the world. As the most sought-after organ for transplantation, the kidney is of immense importance in the field of tissue engineering. The primary obstacle to the development of clinically relevant tissue engineered kidneys is precise vascularization due to the organ’s large size and complexity. Current attempts at whole-kidney tissue engineering include the repopulation of decellularized kidney extracellular matrices or vascular corrosion casts, but these approaches do not eliminate the need for a donor organ. Stem cell-based approaches, such as kidney organoids vascularized in microphysiological systems, aim to construct a kidney without the need for organ donation. These organ-on-a-chip models show complex, functioning kidney structures, albeit at a small scale. Novel methodologies for developing engineered scaffolds will allow for improved differentiation of kidney stem cells and organoids into larger kidney grafts with clinical applications. While currently, kidney tissue engineering remains mostly limited to individual renal structures or small organoids, further developments in vascularization techniques, with technologies such as organoids in microfluidic systems, could potentially open doors for a large-scale growth of whole engineered kidneys for transplantation.

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Characterization of protein release from photocrosslinkable hyaluronic acid-polyethylene glycol hydrogel tissue engineering scaffolds

Biomaterials ◽

10.1016/j.biomaterials.2004.02.018 ◽

2005 ◽

Vol 26 (2) ◽

pp. 125-135 ◽

Cited By ~ 307

Author(s):

Jennie B Leach ◽

Christine E Schmidt

Keyword(s):

Tissue Engineering ◽

Hyaluronic Acid ◽

Polyethylene Glycol ◽

Protein Release ◽

Tissue Engineering Scaffolds

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Cell-Laden Gelatin Methacryloyl Bioink for the Fabrication of Z-Stacked Hydrogel Scaffolds for Tissue Engineering

Polymers ◽

10.3390/polym12123027 ◽

2020 ◽

Vol 12 (12) ◽

pp. 3027

Author(s):

Jeong Wook Seo ◽

Joon Ho Moon ◽

Goo Jang ◽

Woo Kyung Jung ◽

Yong Ho Park ◽

...

Keyword(s):

Electron Microscopy ◽

Tissue Engineering ◽

Present Method ◽

High Speed ◽

Light Exposure ◽

Hydrogel Scaffolds ◽

Time Points ◽

Large Size ◽

Internal Morphology ◽

Complex Morphology

Hydrogel-based scaffolds have been widely used to fabricate artificial tissues capable of replacing tissues and organs. However, several challenges inherent in fabricating tissues of large size and complex morphology using such scaffolds while ensuring cell viability remain. To address this problem, we synthesized gelatin methacryloyl (GelMA) based bioink with cells for fabricating a scaffold with superior characteristics. The bioink was grafted onto a Z-stacking bioprinter that maintained the cells at physiological temperature during the printing process, without exerting any physical pressure on the cells. Various parameters, such as the bioink composition and light exposure time, were optimized. The printing accuracy of the scaffolds was evaluated using photorheological studies. The internal morphology of the scaffolds at different time points was analyzed using electron microscopy. The Z-stacked scaffolds were fabricated using high-speed printing, with the conditions optimized to achieve high model reproducibility. Stable adhesion and high proliferation of cells encapsulated within the scaffold were confirmed. We introduced various strategies to improve the accuracy and reproducibility of Z-stack GelMA bioprinting while ensuring that the scaffolds facilitated cell adhesion, encapsulation, and proliferation. Our results demonstrate the potential of the present method for various applications in tissue engineering.

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ENCAPSULATION AND RELEASE PROFILE OF PROTEIN CAGE FROM A POLYMERIC MATRIX

Nano LIFE ◽

10.1142/s1793984411000347 ◽

2012 ◽

Vol 02 (01) ◽

pp. 1250001 ◽

Cited By ~ 2

Author(s):

YAN LI ◽

RIKE OKTAVIANTI TOYIP ◽

TAO PENG ◽

SIERIN LIM

Keyword(s):

Aqueous Phase ◽

Drug Carrier ◽

Polymer Concentration ◽

Ionic Concentration ◽

Release Profile ◽

Protein Release ◽

Cage Structure ◽

E2 Protein ◽

Microparticle Size ◽

Plga Microparticles

Protein cages have been widely investigated as molecular drug carrier. E2 protein from Bacillus stearothermophillus forms a dodecahedral cage structure of approximately 24 nm in diameter. To formulate a sustainable release profile, E2 protein was further encapsulated into poly(lactide-co-glycolide) (PLGA) microparticles to form a composite structure using water-in-oil-in-water (W/O/W) double emulsion method. The influence of fabrication parameters on microparticle morphology and E2 protein release profile were investigated. The microparticle size increased when the stirring speed of the second emulsification decreased. Decrease in the volume of external aqueous phase led to the reduction of microparticle size without affecting its porosity. The higher ionic concentration of external aqueous phase in the presence of surfactant resulted in microparticles with closed pores on surface. Increase in polymer concentration also led to the formation of less porous microparticles. The E2 protein was not dissociated upon encapsulation into PLGA microparticles based on the unchanged particle size of E2 protein. E2 protein release was studied in phosphate-buffered saline solution at 37°C. The initial burst and release rate were lowered as the surfactant concentration in external water phase during the fabrication process was increased from 0.1% to 1% (w/v). After 14-day incubation, no observable polymer degradation was found while the surface of microparticles appeared to be smoother than before incubation.

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