Factorial and ‘self vs. other’ plant soil feedback experiments produce similar predictions of plant growth in communities

AbstractBackgroundSince the dawn of agriculture, human selection on plants has progressively differentiated input-demanding productive crops from their wild progenitors thriving in marginal areas. Barley (Hordeum vulgare), the fourth most cultivated cereal globally, is a prime example of this process. We previously demonstrated that wild and domesticated barley genotypes host distinct microbial communities in their rhizosphere. Here, we tested the hypothesis that microbiota diversification is modulated by, and responds to, nitrogen (N) application in soil and assessed the impact of microbiota taxonomic and functional compositions on plant growth.MethodsWe grew two wild (H. vulgare ssp. spontaneum) and an ‘Elite’ domesticated (H. vulgare ssp. vulgare) barley genotypes in an agricultural soil treated with and without N inputs. By using a two-pronged 16S rRNA gene amplicon sequencing and comparative metagenomics approach, we determined the impact of N application on taxonomic composition and metabolic potential of the microbial communities exposed to limiting and replete N supplies. We then implemented a plant-soil feedback experiment to assess microbiotas’ recruitment cues and contribution to plant growth.ResultsN availability emerged as a modulator of the recruitment cues of the barley bacterial microbiota as evidenced by the increased number of bacterial genera differentially recruited between unplanted soil and rhizosphere communities under N-limiting conditions. This recruitment pattern mirrored the impact of the host genotype on rhizosphere bacteria. The characterisation of the assembled metagenomes of plants exposed to N-limiting conditions revealed a metabolic specialisation of the rhizosphere microbiota compared to unplanted soil controls. This specialisation is underpinned predominantly by bacteria and is manifested by the enrichment of a core set of biological processes sustaining the adaptation of polymicrobial communities such as N utilisation, quorum sensing and motility across genotypes. The quantitative variation in a group of these biological processes defined host signatures in the barley rhizosphere metagenome. Finally, a plant-soil feedback experiment revealed that the host-mediated taxonomic diversification of the bacterial microbiota is associated with barley growth under sub-optimal N supplies.ConclusionsOur results suggest that under N limiting conditions, a substrate-driven selection process underpins the assembly of barley rhizosphere microbiota. Host-microbe and microbe-microbe interactions fine-tune this process at the taxonomic and functional level across kingdoms. The disruption of these recruitment cues negatively impacts plant growth.

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Soil microorganism impact on the native plant growth under single and co-invasion with invasive plants: Responses through plant-soil feedback

Biomedical Letters ◽

10.47262//bl/6.2.20200432 ◽

2020 ◽

Vol 6 (2) ◽

pp. 104-111

Keyword(s):

Plant Growth ◽

Invasive Plants ◽

Plant Invasion ◽

Soil Microorganisms ◽

Invasive Plant ◽

Plant Competition ◽

Soil Microorganism ◽

Native Plant ◽

Plant Soil ◽

Soil Feedback

Plant invasion is a key element defining the community structure and dynamics and has become a major concern for the invasive plants to control the restoration of ecosystem diversity. In the same line of thought, soil microorganisms are also considered as a significant parameter of evolution and invasive plants' success. The variations usually overserved in the composition and structure of the soil microorganisms and the consequences of plant invasion. Therefore, understanding the concept of plant invasion and soil microorganism impact plant competition and plant-soil feedback would be a very important step forward in invasive plant control and ecosystem restoration. This review aims to provide a conceptual explanation of plant invasion, the role of soil microorganisms on plant growth and its effects on the native plant-soil feedback and also to demonstrate the importance of understanding the integrative soil microorganism impact on the competition between native and invasive plants along with its effects on plant-soil feedback.

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Faculty Opinions recommendation of Are plant-soil feedback responses explained by plant traits?

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718479822.793497714 ◽

2014 ◽

Author(s):

Christian Körner

Keyword(s):

Plant Traits ◽

Plant Soil ◽

Soil Feedback ◽

Feedback Responses

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Negative Plant-Soil Feedback Driven by Re-assemblage of the Rhizosphere Microbiome With the Growth of Panax notoginseng

Frontiers in Microbiology ◽

10.3389/fmicb.2019.01597 ◽

2019 ◽

Vol 10 ◽

Cited By ~ 6

Author(s):

Lifen Luo ◽

Cunwu Guo ◽

Luotao Wang ◽

Junxing Zhang ◽

Linmei Deng ◽

...

Keyword(s):

Panax Notoginseng ◽

Rhizosphere Microbiome ◽

Plant Soil ◽

Soil Feedback

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The importance of species phylogenetic relationships and species traits for the intensity of plant-soil feedback

Ecosphere ◽

10.1890/es15-00206.1 ◽

2015 ◽

Vol 6 (11) ◽

pp. art234 ◽

Cited By ~ 19

Author(s):

Z. Münzbergová ◽

M. Šurinová

Keyword(s):

Phylogenetic Relationships ◽

Species Traits ◽

Plant Soil ◽

Soil Feedback

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Effects of crop rotations on microbial community in rhizosphere soil of cucumber seedlings and its feedback

Allelopathy Journal ◽

10.26651/allelo.j/2021-52-2-1318 ◽

2021 ◽

Vol 52 (2) ◽

pp. 239-250

Author(s):

X.J. He ◽

W.W. Zhu ◽

F.Z. Wu

Keyword(s):

Soil Fungi ◽

Dry Weight ◽

Crop Rotations ◽

Kidney Bean ◽

Absolute Abundance ◽

Cucumber Seedlings ◽

Soil Microbial ◽

Plant Soil ◽

Significant Difference ◽

Soil Feedback

We studied the effects of 7-crop rotations and continuous - monocropping systems on soil microorganism and its feedback. The results showed that absolute abundance of soil bacteria (Pseudomonas and Bacillus) in tomato - celery - cucumber - cabbage and cucumber - tomato - cucumber - cabbage rotation were significantly higher than control (CK). Absolute abundance of soil fungi in tomato - celery - cucumber - cabbage, kidney bean - celery - cucumber - cabbage, cucumber - kidney bean - cucumber - cabbage and cucumber - tomato - cucumber - cabbage rotation were significantly higher than CK. Dry weight of cucumber seedlings was significantly positively correlated with bacterial (Pseudomonas and Bacillus) abundance, and negatively correlated with fungal count. The results of inoculation with Fusarium oxysporum f.sp. cucumerinum showed that plant dry weight of cucumber seedlings in tomato - celery - cucumber - cabbage, cucumber - kidney bean - cucumber - cabbage, cucumber - tomato - cucumber - cabbage rotation soil was significantly higher than other treatments, and their disease index was significantly lower than other treatments. There was no significant difference in dry weight of cucumber seedlings in rotation and CK in the soil sterilization test. The results of plant - soil feedback experiment showed that soil microbial changes caused by different rotation patterns had a positive feedback effect on growth of cucumber seedlings.

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Wetland eco-engineering: measuring and modeling feedbacks of oxidation processes between plants and clay-rich material

Biogeosciences ◽

10.5194/bg-13-4945-2016 ◽

2016 ◽

Vol 13 (17) ◽

pp. 4945-4957 ◽

Cited By ~ 6

Author(s):

Rémon Saaltink ◽

Stefan C. Dekker ◽

Jasper Griffioen ◽

Martin J. Wassen

Keyword(s):

Plant Growth ◽

Feedback Loop ◽

Pyrite Oxidation ◽

Iron Toxicity ◽

Biogeochemical Processes ◽

Engineering Project ◽

Feedback Mechanisms ◽

Plant Soil ◽

Water Composition ◽

Rich Material

Abstract. Interest is growing in using soft sediment as a foundation in eco-engineering projects. Wetland construction in the Dutch lake Markermeer is an example: here, dredging some of the clay-rich lake-bed sediment and using it to construct wetland will soon begin. Natural processes will be utilized during and after construction to accelerate ecosystem development. Knowing that plants can eco-engineer their environment via positive or negative biogeochemical plant–soil feedbacks, we conducted a 6-month greenhouse experiment to identify the key biogeochemical processes in the mud when Phragmites australis is used as an eco-engineering species. We applied inverse biogeochemical modeling to link observed changes in pore water composition to biogeochemical processes. Two months after transplantation we observed reduced plant growth and shriveling and yellowing of foliage. The N : P ratios of the plant tissue were low, and these were affected not by hampered uptake of N but by enhanced uptake of P. Subsequent analyses revealed high Fe concentrations in the leaves and roots. Sulfate concentrations rose drastically in our experiment due to pyrite oxidation; as reduction of sulfate will decouple Fe-P in reducing conditions, we argue that plant-induced iron toxicity hampered plant growth, forming a negative feedback loop, while simultaneously there was a positive feedback loop, as iron toxicity promotes P mobilization as a result of reduced conditions through root death, thereby stimulating plant growth and regeneration. Given these two feedback mechanisms, we propose the use of Fe-tolerant species rather than species that thrive in N-limited conditions. The results presented in this study demonstrate the importance of studying the biogeochemical properties of the situated sediment and the feedback mechanisms between plant and soil prior to finalizing the design of the eco-engineering project.

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