scholarly journals Nitrogen availability modulates the host control of the barley rhizosphere microbiota

2019 ◽  
Author(s):  
Rodrigo Alegria Terrazas ◽  
Senga Robertson-Albertyn ◽  
Aileen Mary Corral ◽  
Carmen Escudero-Martinez ◽  
Katharin Balbirnie-Cumming ◽  
...  
Keyword(s):  
Plant Growth ◽  
Microbial Communities ◽  
Biological Processes ◽  
N Application ◽  
Plant Soil ◽  
Unplanted Soil ◽  
Bacterial Microbiota ◽  
Soil Feedback ◽  
The Impact ◽  
Barley Genotypes

AbstractBackgroundSince the dawn of agriculture, human selection on plants has progressively differentiated input-demanding productive crops from their wild progenitors thriving in marginal areas. Barley (Hordeum vulgare), the fourth most cultivated cereal globally, is a prime example of this process. We previously demonstrated that wild and domesticated barley genotypes host distinct microbial communities in their rhizosphere. Here, we tested the hypothesis that microbiota diversification is modulated by, and responds to, nitrogen (N) application in soil and assessed the impact of microbiota taxonomic and functional compositions on plant growth.MethodsWe grew two wild (H. vulgare ssp. spontaneum) and an ‘Elite’ domesticated (H. vulgare ssp. vulgare) barley genotypes in an agricultural soil treated with and without N inputs. By using a two-pronged 16S rRNA gene amplicon sequencing and comparative metagenomics approach, we determined the impact of N application on taxonomic composition and metabolic potential of the microbial communities exposed to limiting and replete N supplies. We then implemented a plant-soil feedback experiment to assess microbiotas’ recruitment cues and contribution to plant growth.ResultsN availability emerged as a modulator of the recruitment cues of the barley bacterial microbiota as evidenced by the increased number of bacterial genera differentially recruited between unplanted soil and rhizosphere communities under N-limiting conditions. This recruitment pattern mirrored the impact of the host genotype on rhizosphere bacteria. The characterisation of the assembled metagenomes of plants exposed to N-limiting conditions revealed a metabolic specialisation of the rhizosphere microbiota compared to unplanted soil controls. This specialisation is underpinned predominantly by bacteria and is manifested by the enrichment of a core set of biological processes sustaining the adaptation of polymicrobial communities such as N utilisation, quorum sensing and motility across genotypes. The quantitative variation in a group of these biological processes defined host signatures in the barley rhizosphere metagenome. Finally, a plant-soil feedback experiment revealed that the host-mediated taxonomic diversification of the bacterial microbiota is associated with barley growth under sub-optimal N supplies.ConclusionsOur results suggest that under N limiting conditions, a substrate-driven selection process underpins the assembly of barley rhizosphere microbiota. Host-microbe and microbe-microbe interactions fine-tune this process at the taxonomic and functional level across kingdoms. The disruption of these recruitment cues negatively impacts plant growth.

scholarly journals An Affinity–Effect Relationship for Microbial Communities in Plant–Soil Feedback Loops

2014 ◽  
Vol 67 (4) ◽  
pp. 866-876 ◽  
Author(s):  
Yi Lou ◽  
Sharon A. Clay ◽  
Adam S. Davis ◽  
Anita Dille ◽  
Joel Felix ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Feedback Loops ◽  
Effect Relationship ◽  
Plant Soil ◽  
Soil Feedback

scholarly journals Microbial Inoculants and Their Impact on Soil Microbial Communities: A Review

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Darine Trabelsi ◽  
Ridha Mhamdi
Keyword(s):  
Microbial Communities ◽  
Soil Microbial Communities ◽  
Future Research ◽  
Bacterial Strains ◽  
Microbial Inoculants ◽  
Soil Microbial ◽  
Functional Capabilities ◽  
Plant Soil ◽  
Taxonomic Groups ◽  
The Impact

The knowledge of the survival of inoculated fungal and bacterial strains in field and the effects of their release on the indigenous microbial communities has been of great interest since the practical use of selected natural or genetically modified microorganisms has been developed. Soil inoculation or seed bacterization may lead to changes in the structure of the indigenous microbial communities, which is important with regard to the safety of introduction of microbes into the environment. Many reports indicate that application of microbial inoculants can influence, at least temporarily, the resident microbial communities. However, the major concern remains regarding how the impact on taxonomic groups can be related to effects on functional capabilities of the soil microbial communities. These changes could be the result of direct effects resulting from trophic competitions and antagonistic/synergic interactions with the resident microbial populations, or indirect effects mediated by enhanced root growth and exudation. Combination of inoculants will not necessarily produce an additive or synergic effect, but rather a competitive process. The extent of the inoculation impact on the subsequent crops in relation to the buffering capacity of the plant-soil-biota is still not well documented and should be the focus of future research.

Soil microbial communities influence seedling growth of a rare conifer independent of plant-soil feedback

Ecology ◽  
2016 ◽  
Vol 97 (12) ◽  
pp. 3346-3358 ◽  
Author(s):  
Jessica L. Rigg ◽  
Cathy A. Offord ◽  
Brajesh K. Singh ◽  
Ian Anderson ◽  
Steve Clarke ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Seedling Growth ◽  
Soil Microbial Communities ◽  
Soil Microbial ◽  
Plant Soil ◽  
Soil Feedback

scholarly journals Fertilizer N application rate impacts plant-soil feedback in a sanqi production system

2018 ◽  
Vol 633 ◽  
pp. 796-807 ◽  
Author(s):  
Wei Wei ◽  
Min Yang ◽  
Yixiang Liu ◽  
Huichuan Huang ◽  
Chen Ye ◽  
...  
Keyword(s):  
Production System ◽  
Application Rate ◽  
Fertilizer N ◽  
N Application ◽  
Plant Soil ◽  
N Application Rate ◽  
Soil Feedback

Soil microorganism impact on the native plant growth under single and co-invasion with invasive plants: Responses through plant-soil feedback

2020 ◽  
Vol 6 (2) ◽  
pp. 104-111
Keyword(s):  
Plant Growth ◽  
Invasive Plants ◽  
Plant Invasion ◽  
Soil Microorganisms ◽  
Invasive Plant ◽  
Plant Competition ◽  
Soil Microorganism ◽  
Native Plant ◽  
Plant Soil ◽  
Soil Feedback

Plant invasion is a key element defining the community structure and dynamics and has become a major concern for the invasive plants to control the restoration of ecosystem diversity. In the same line of thought, soil microorganisms are also considered as a significant parameter of evolution and invasive plants' success. The variations usually overserved in the composition and structure of the soil microorganisms and the consequences of plant invasion. Therefore, understanding the concept of plant invasion and soil microorganism impact plant competition and plant-soil feedback would be a very important step forward in invasive plant control and ecosystem restoration. This review aims to provide a conceptual explanation of plant invasion, the role of soil microorganisms on plant growth and its effects on the native plant-soil feedback and also to demonstrate the importance of understanding the integrative soil microorganism impact on the competition between native and invasive plants along with its effects on plant-soil feedback.

scholarly journals Effects of Grazing Intensity on Plant-Soil C: N: P Stoichiometry with Precipitation Changes During the Growing Season in Desert Grassland

2021 ◽  
Author(s):  
Wei Yan ◽  
Fengling Shi ◽  
Tao Wan
Keyword(s):  
Species Diversity ◽  
Plant Growth ◽  
Grazing Intensity ◽  
Desert Grassland ◽  
C:N:P Stoichiometry ◽  
Soil C ◽  
Plant Soil ◽  
Heavy Grazing ◽  
Significant Difference ◽  
The Impact

Abstract Background and aimsHigh-intensity grazing in the Mongolian grassland has led to the general deterioration of biodiversity and ecosystem functioning. Although abundant evidence shows that grazing affects the structure and function of grassland ecosystems, research on the impact of precipitation, especially under drought and overgrazing. MethodsWe examined the effects of heavy grazing, moderate grazing and no grazing on plant communities; plant and soil C, N and P contents; and plant and soil C:N:P stoichiometry in the desert grassland in different years with different amounts of precipitation. ResultsThere was no significant difference in the species diversity between the grazing and no grazing treatment, while the no grazing treatment was significantly higher than the heavy grazing treatment. Compared with the amounts in the no grazing and moderate grazing treatments, the N and P contents of the plants in the heavy grazing treatment were the highest, and the N content of the soil increased. There was a positive correlation between precipitation and the N and P contents of plants and the C and N contents of the soil at 0-10 cm and 10-20 cm. ConclusionsOur study suggest that a large amount of precipitation of plant growth will drive changes in the community species diversity. Grazing promoted the flow of N between plants and the soil, especially under heavy grazing. Under grazing stress, plants maintain the potential of compensatory growth, and precipitation in the peak season of plant growth induces rapid growth, suggesting that precipitation is an important factor driving grazing ecosystems.

scholarly journals A footprint of plant eco-geographic adaptation on the composition of the barley rhizosphere bacterial microbiota

2020 ◽  
Author(s):  
Rodrigo Alegria Terrazas ◽  
Katharin Balbirnie-Cumming ◽  
Jenny Morris ◽  
Pete E Hedley ◽  
Joanne Russell ◽  
...  
Keyword(s):  
Wild Barley ◽  
Critical Role ◽  
Crop Wild Relatives ◽  
Genomic Diversity ◽  
Rrna Gene ◽  
Genotype Effect ◽  
Bacterial Microbiota ◽  
Evolutionary Trajectories ◽  
The Impact ◽  
Barley Genotypes

AbstractThe microbiota thriving in the rhizosphere, the thin layer of soil surrounding plant roots, plays a critical role in plant’s adaptation to the environment. Domestication and breeding selection have progressively differentiated the microbiota of modern crops from the ones of their wild ancestors. However, the impact of eco-geographical constraints faced by domesticated plants and crop wild relatives on recruitment and maintenance of the rhizosphere microbiota remains to be fully elucidated. Here we performed a comparative 16S rRNA gene survey of the rhizosphere of 4 domesticated and 20 wild barley (Hordeum vulgare) genotypes grown in an agricultural soil under controlled environmental conditions. We demonstrated the enrichment of individual bacteria mirrored the distinct eco-geographical constraints faced by their host plants. Unexpectedly, Elite varieties exerted a stronger genotype effect on the rhizosphere microbiota when compared with wild barley genotypes adapted to desert environments with a preferential enrichment for members of Actinobacteria. Finally, in wild barley genotypes, we discovered a limited, but significant, correlation between microbiota diversity and host genomic diversity. Our results revealed a footprint of the host’s adaptation to the environment on the assembly of the bacteria thriving at the root-soil interface. In the tested conditions, this recruitment cue layered atop of the distinct evolutionary trajectories of wild and domesticated plants and, at least in part, is encoded by the barley genome. This knowledge will be critical to design experimental approaches aimed at elucidating the recruitment cues of the barley microbiota across a range of soil types.

Fluorescent potentiometric dyes for membrane-potential imaging

1994 ◽  
Vol 52 ◽  
pp. 166-167
Author(s):  
Leslie M. Loew
Keyword(s):  
Membrane Potential ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Optical Recording ◽  
Biological Processes ◽  
Major Focus ◽  
The Past ◽  
Excitable Systems ◽  
Major Application ◽  
The Impact

A major application of potentiometric dyes has been the multisite optical recording of electrical activity in excitable systems. After being championed by L.B. Cohen and his colleagues for the past 20 years, the impact of this technology is rapidly being felt and is spreading to an increasing number of neuroscience laboratories. A second class of experiments involves using dyes to image membrane potential distributions in single cells by digital imaging microscopy - a major focus of this lab. These studies usually do not require the temporal resolution of multisite optical recording, being primarily focussed on slow cell biological processes, and therefore can achieve much higher spatial resolution. We have developed 2 methods for quantitative imaging of membrane potential. One method uses dual wavelength imaging of membrane-staining dyes and the other uses quantitative 3D imaging of a fluorescent lipophilic cation; the dyes used in each case were synthesized for this purpose in this laboratory.

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